{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T02:43:05Z","timestamp":1773283385336,"version":"3.50.1"},"reference-count":19,"publisher":"Microbiology Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,6,1]]},"abstract":"<jats:p>The serotypes and molecular clones of penicillin-nonsusceptible <jats:italic>Streptococcus pneumoniae<\/jats:italic> (PNSP) responsible for invasive pneumococcal disease (IPD) and discharging ears in metropolitan New South Wales were characterized to form a baseline prior to introduction of the heptavalent conjugate pneumococcal vaccine in Australia. Pneumococci isolated between 1 July 2000 and 30 June 2003 in Sydney from children &lt;15 years were tested for antibiotic susceptibilities and serotyped. Penicillin-nonsusceptible pneumococci were typed by multilocus sequence typing and BOX PCR. During this period, 97 (13.9\u200a%) of 698 pneumococci from IPD that were serotyped were penicillin-nonsusceptible. Of 607 pneumococci from discharging ears, 157 (26.1\u200a%) were penicillin-nonsusceptible. Serotype 14 was the predominant serotype responsible for IPD and serotype 19F predominated from discharging ears. The heptavalent vaccine serotypes accounted for 613 (87.8\u200a%) of all invasive isolates and 420 (69.8\u200a%) of all isolates from discharging ears. Representatives of the major international clones were present among the PNSP. The majority of serotypes and clones that showed penicillin-nonsusceptibility are present within the vaccine. Serotype switching was also noted to have occurred prior to introduction of the vaccine. This study provides a characterization of the pneumococcal serotypes associated with IPD and discharging ears that will be useful for detecting potential selective effects of the vaccine. This surveillance should be continued, as it will be important to monitor the frequency and distribution of serotypes in the post-vaccine era.<\/jats:p>","DOI":"10.1099\/jmm.0.47057-0","type":"journal-article","created":{"date-parts":[[2007,5,17]],"date-time":"2007-05-17T22:41:59Z","timestamp":1179441719000},"page":"819-823","source":"Crossref","is-referenced-by-count":6,"title":["Pneumococci responsible for invasive disease and discharging ears in children in Sydney, Australia"],"prefix":"10.1099","volume":"56","author":[{"given":"Michael","family":"Watson","sequence":"first","affiliation":[{"name":"The New South Wales Pneumococcal Reference Laboratory, The Children\u2019s Hospital at Westmead, Department of Microbiology, Westmead, New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maggie","family":"Brett","sequence":"additional","affiliation":[{"name":"The New South Wales Pneumococcal Reference Laboratory, The Children\u2019s Hospital at Westmead, Department of Microbiology, Westmead, New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitchell","family":"Brown","sequence":"additional","affiliation":[{"name":"The New South Wales Pneumococcal Reference Laboratory, The Children\u2019s Hospital at Westmead, Department of Microbiology, Westmead, New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marianne G.","family":"Stewart","sequence":"additional","affiliation":[{"name":"The New South Wales Pneumococcal Reference Laboratory, The Children\u2019s Hospital at Westmead, Department of Microbiology, Westmead, New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shirley","family":"Warren","sequence":"additional","affiliation":[{"name":"The New South Wales Pneumococcal Reference Laboratory, The Children\u2019s Hospital at Westmead, Department of Microbiology, Westmead, New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"for the New South Wales Pneumococcal Network","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"issue":"Suppl. 2","key":"R1","doi-asserted-by":"crossref","first-page":"S127","DOI":"10.1007\/s00431-002-1064-z","article-title":"Safety and efficacy of the seven-valent pneumococcal conjugate vaccine: evidence from Northern California","volume":"161","author":"Black","year":"2002","journal-title":"Eur J Pediatr"},{"key":"R2","doi-asserted-by":"crossref","first-page":"4449","DOI":"10.1128\/JCM.42.10.4449-4452.2004","article-title":"Serotypes and sequence types of pneumococci causing invasive disease in Scotland prior to the introduction of pneumococcal conjugate polysaccharide vaccines","volume":"42","author":"Clarke","year":"2004","journal-title":"J Clin Microbiol"},{"key":"R3","doi-asserted-by":"crossref","first-page":"3049","DOI":"10.1099\/00221287-144-11-3049","article-title":"A multilocus sequence typing scheme for Streptococcus pneumoniae : identification of clones associated with serious invasive disease","volume":"144","author":"Enright","year":"1998","journal-title":"Microbiology"},{"key":"R4","first-page":"403","article-title":"Efficacy of a pneumococcal conjugate vaccine against acute otitis media","volume":"344","author":"Eskola","year":"2001","journal-title":"Finnish Otitis Media Study Group.. 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