{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T12:01:34Z","timestamp":1771588894516,"version":"3.50.1"},"reference-count":26,"publisher":"Microbiology Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,10,1]]},"abstract":"<jats:p>A proportion of patients with chronic rhinosinusitis, especially if nasal polyps are present, have a diagnosis of fungal rhinosinusitis. The diagnosis is difficult to establish because the symptoms and clinical and radiological signs are non-specific. Also current diagnostic methods, i.e. histology, fungal staining and culture, are insensitive. The performance of the<jats:italic>Aspergillus<\/jats:italic>galactomannan (GM) ELISA and real-time PCR for<jats:italic>Aspergillus fumigatus<\/jats:italic>mitochondrial DNA was evaluated for the detection of<jats:italic>Aspergillus<\/jats:italic>in sinus mucus samples from 25 patients with chronic rhinosinusitis with nasal polyposis. The results were compared with those from nasal lavage fluid from 19 healthy volunteers. Seven patients (28\u200a%) were diagnosed as having fungal rhinosinusitis according to the presence of filaments in histology or direct microscopy using Calcofluor white. All fungal rhinosinusitis patients were negative in the GM ELISA. GM ELISA was positive in five patients whose samples were negative using conventional methods and<jats:italic>A. fumigatus<\/jats:italic>PCR. Two out of seven patients with fungal rhinosinusitis were positive by<jats:italic>A. fumigatus<\/jats:italic>PCR: one also had a positive<jats:italic>A. fumigatus<\/jats:italic>culture, and one had hyphae consistent with<jats:italic>Aspergillus<\/jats:italic>in histology. One additional patient had a weak positive PCR result, but other fungal tests were negative. In control subjects, the GM ELISA was positive in 21\u200a%, whereas direct microscopy, culture and<jats:italic>A. fumigatus<\/jats:italic>PCR were negative in all samples. Direct microscopy and culture together with histology remain pivotal in defining fungal rhinosinusitis diagnosis.<jats:italic>A. fumigatus<\/jats:italic>PCR may have additional value in allowing the diagnosis to be made sooner, whereas the GM ELISA is not reliable in diagnosing<jats:italic>Aspergillus<\/jats:italic>infection of the paranasal sinuses.<\/jats:p>","DOI":"10.1099\/jmm.0.47101-0","type":"journal-article","created":{"date-parts":[[2007,9,24]],"date-time":"2007-09-24T17:02:45Z","timestamp":1190653365000},"page":"1322-1327","source":"Crossref","is-referenced-by-count":14,"title":["Negative impact of Aspergillus galactomannan and DNA detection in the diagnosis of fungal rhinosinusitis"],"prefix":"10.1099","volume":"56","author":[{"given":"Katriina","family":"Kostamo","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology, Kymenlaakso Central Hospital, Kotka, Finland"},{"name":"Department of Otorhinolaryngology, Helsinki University Central Hospital, 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