{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T11:02:38Z","timestamp":1790938958745,"version":"4.1.0"},"reference-count":30,"publisher":"Microbiology Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,11,1]]},"abstract":"<jats:p>Shiga toxins (Stx1 and Stx2) are responsible for initiating haemolytic uraemic syndrome, a serious extraintestinal complication caused by enterohaemorrhagic<jats:italic>Escherichia coli<\/jats:italic>O157\u200a:\u200aH7 infection in humans. Shiga toxins are classical AB<jats:sub>5<\/jats:sub>-type exotoxins, consisting of a globotriaosylceramide (Gb<jats:sub>3<\/jats:sub>)-binding B subunit pentamer and an enzymic A subunit. It is demonstrated in this study that Stx2 binds to human neutrophils by a non-classical mechanism that is independent of Gb<jats:sub>3<\/jats:sub>. In contrast, the investigation revealed that Stx2 binds to murine neutrophils by the classical Gb<jats:sub>3<\/jats:sub>-dependent mechanism. Moreover, whereas the human serum amyloid P (HuSAP) component inhibited Stx2 binding to murine neutrophils, HuSAP increased Stx2 binding to human neutrophils by 84.2\u200a% (<jats:italic>P<\/jats:italic>\u22640.002, Student's<jats:italic>t<\/jats:italic>-test). These observations may explain why HuSAP protects mice from the lethal effects of Stx2, whereas there is no indication that HuSAP plays a similar protective role in humans infected by<jats:italic>E. coli<\/jats:italic>O157\u200a:\u200aH7.<\/jats:p>","DOI":"10.1099\/jmm.0.47282-0","type":"journal-article","created":{"date-parts":[[2007,10,26]],"date-time":"2007-10-26T18:24:52Z","timestamp":1193423092000},"page":"1423-1430","source":"Crossref","is-referenced-by-count":34,"title":["Differential binding of Shiga toxin 2 to human and murine neutrophils"],"prefix":"10.1099","volume":"56","author":[{"given":"Thomas P.","family":"Griener","sequence":"first","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, Calgary, Alberta T2N 4N1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George L.","family":"Mulvey","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, Calgary, Alberta T2N 4N1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Marcato","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Sir Charles Tupper Medical Building, Dalhousie University, Halifax, Nova Scotia B3H 1X5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glen D.","family":"Armstrong","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Centre, Calgary, Alberta T2N 4N1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.1086\/501472","article-title":"Human serum amyloid P component protects against Escherichia coli O157\u200a:\u200aH7 Shiga toxin 2 in vivo : therapeutic implications for hemolytic-uremic syndrome","volume":"193","author":"Armstrong","year":"2006","journal-title":"J Infect Dis"},{"key":"R2","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1128\/JCM.42.3.1099-1108.2004","article-title":"Characterization of Shiga toxin-producing Escherichia coli strains isolated from human patients in Germany over a 3-year period","volume":"42","author":"Beutin","year":"2004","journal-title":"J Clin Microbiol"},{"key":"R3","doi-asserted-by":"crossref","first-page":"3337","DOI":"10.1128\/IAI.62.8.3337-3347.1994","article-title":"Evidence that Verotoxins (Shiga-like toxins) from Escherichia coli bind to P blood group antigens of human erythrocytes in vitro","volume":"62","author":"Bitzan","year":"1994","journal-title":"Infect Immun"},{"key":"R4","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1128\/JCM.44.2.313-317.2006","article-title":"Shiga toxins present in the gut and in the polymorphonuclear leukocytes circulating in the blood of children with hemolytic-uremic syndrome","volume":"44","author":"Brigotti","year":"2006","journal-title":"J Clin Microbiol"},{"key":"R5","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/S0378-1097(03)00761-4","article-title":"Shiga toxins and apoptosis","volume":"228","author":"Cherla","year":"2003","journal-title":"FEMS Microbiol Lett"},{"key":"R6","doi-asserted-by":"crossref","first-page":"1767","DOI":"10.1152\/jappl.1990.69.5.1767","article-title":"Retention of leukocytes in capillaries: role of cell size and deformability","volume":"69","author":"Downey","year":"1990","journal-title":"J Appl Physiol"},{"key":"R7","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1128\/IAI.01594-06","article-title":"Comparative analysis of the abilities of Shiga toxins 1 and 2 to bind to and influence neutrophil apoptosis","volume":"75","author":"Flagler","year":"2007","journal-title":"Infect Immun"},{"key":"R8","doi-asserted-by":"crossref","first-page":"27511","DOI":"10.1074\/jbc.M401939200","article-title":"Structure of Shiga toxin type 2 (Stx2) from Escherichia coli O157\u200a:\u200aH7","volume":"279","author":"Fraser","year":"2004","journal-title":"J Biol Chem"},{"key":"R9","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1016\/S0021-9258(20)71209-3","article-title":"Structures of glycosphingolipids isolated from human granulocytes. 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