{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T23:07:39Z","timestamp":1719270459156},"reference-count":22,"publisher":"Microbiology Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,12,1]]},"abstract":"\n Campylobacter<\/jats:italic> \n jejuni<\/jats:italic> strains develop a high variability of lipooligosaccharide (LOS) structures on the cell surface based on variations in the genetic content of the LOS biosynthesis locus. While the importance of these variations for ganglioside mimicry as a critical factor in the triggering of Guillain\u2013Barr\u00e9 syndrome has already been shown, little work has been done on the investigation of LOS structures and their function in the pathogenesis of gastrointestinal disease. In this study, the presence of several LOS genes in 40 C. jejuni<\/jats:italic> strains with different abilities to colonize the chicken gut and to invade Caco-2 cells was investigated by PCR. Two genes, cgtB<\/jats:italic> and wlaN<\/jats:italic>, encoding putative \u03b2<\/jats:italic>-1,3-galactosyltransferases were detected in most strongly invasive strains and rarely in non-invasive strains. A homopolymeric tract within the wlaN<\/jats:italic> gene resulted in an intact gene product only in strongly invasive strains. The specific function of these genes during LOS biosynthesis is still unknown. cgtB<\/jats:italic> and wlaN<\/jats:italic> gene products are suggested to be involved in development of the colonization and invasion ability of C. jejuni<\/jats:italic>. After a classification of the complete LOS loci, an association between a particular LOS class and colonization and invasion ability of the C. jejuni<\/jats:italic> strain could not be detected. Lack of the pglB<\/jats:italic> gene involved in protein glycosylation in one strain could be responsible for the weak colonization and invasion ability of this strain. There is some evidence that different genetic characteristics were responsible for strong or weak colonization and the invasion ability of C. jejuni<\/jats:italic> strains.<\/jats:p>","DOI":"10.1099\/jmm.0.47305-0","type":"journal-article","created":{"date-parts":[[2007,11,22]],"date-time":"2007-11-22T18:42:53Z","timestamp":1195756973000},"page":"1589-1594","source":"Crossref","is-referenced-by-count":22,"title":["Comparison of lipooligosaccharide biosynthesis genes of Campylobacter jejuni strains with varying abilities to colonize the chicken gut and to invade Caco-2 cells"],"prefix":"10.1099","volume":"56","author":[{"given":"Jens","family":"M\u00fcller","sequence":"first","affiliation":[{"name":"Friedrich Loeffler Institute, Institute of Bacterial Infections and Zoonoses, Jena, Naumburger Str. 96a, 07743 Jena, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Birgit","family":"Meyer","sequence":"additional","affiliation":[{"name":"Friedrich Loeffler Institute, Institute of Bacterial Infections and Zoonoses, Jena, Naumburger Str. 96a, 07743 Jena, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingrid","family":"H\u00e4nel","sequence":"additional","affiliation":[{"name":"Friedrich Loeffler Institute, Institute of Bacterial Infections and Zoonoses, Jena, Naumburger Str. 96a, 07743 Jena, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helmut","family":"Hotzel","sequence":"additional","affiliation":[{"name":"Friedrich Loeffler Institute, Institute of Bacterial Infections and Zoonoses, Jena, Naumburger Str. 96a, 07743 Jena, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"S103","DOI":"10.1086\/513780","article-title":"Epidemiologic and clinical features of Campylobacter jejuni infections","volume":"176 (Suppl. 2)","author":"Blaser","year":"1997","journal-title":"J Infect Dis"},{"key":"R2","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1046\/j.1365-2958.1996.370890.x","article-title":"Characterization of a post-translational modification of Campylobacter flagellin: identification of a sero-specific glycosyl moiety","volume":"19","author":"Doig","year":"1996","journal-title":"Mol Microbiol"},{"key":"R3","doi-asserted-by":"crossref","first-page":"1706","DOI":"10.1101\/gr.185801","article-title":"Whole genome comparison of Campylobacter jejuni human isolates using a low-cost microarray reveals extensive genetic diversity","volume":"11","author":"Dorrell","year":"2001","journal-title":"Genome Res"},{"key":"R4","doi-asserted-by":"crossref","first-page":"e15","DOI":"10.1371\/journal.pbio.0030015","article-title":"Major structural differences and novel potential virulence mechanisms from the genomes of multiple Campylobacter species","volume":"3","author":"Fouts","year":"2005","journal-title":"PLoS Biol"},{"key":"R5","doi-asserted-by":"crossref","first-page":"2594","DOI":"10.1128\/IAI.68.5.2594-2601.2000","article-title":"The galE gene of Campylobacter jejuni is involved in lipopolysaccharide synthesis and virulence","volume":"68","author":"Fry","year":"2000","journal-title":"Infect Immun"},{"key":"R6","doi-asserted-by":"crossref","first-page":"3896","DOI":"10.1074\/jbc.275.6.3896","article-title":"Biosynthesis of ganglioside mimics in Campylobacter jejuni OH4384. 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