{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:45:52Z","timestamp":1790873152071,"version":"4.1.0"},"reference-count":27,"publisher":"Microbiology Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,11,1]]},"abstract":"<jats:p>In order to investigate the genetic relationships within <jats:italic>Brucella<\/jats:italic> isolated from marine mammals, two genome-based typing methods, variable number of tandem repeats (VNTR) typing and multilocus sequence analysis (MLSA), were applied to a selection of 74 marine mammal isolates. All isolates were examined by VNTR and data were compared with multilocus sequencing data from a subset of 48 of these. Marine mammal brucellae are distinct from classically recognized species by these methods and appear to correspond to three major genetic groups, which reflect distinct preferred hosts. One group contains isolates predominantly found in pinnipeds (seals) and corresponds to the previously proposed species \u2018<jats:italic>Brucella pinnipediae<\/jats:italic>\u2019. However, isolates corresponding to the previously proposed species \u2018<jats:italic>Brucella cetaceae<\/jats:italic>\u2019 fall into two distinct groups that appear to have different preferred cetacean hosts (porpoises and dolphins). Furthermore, these two groups appear less closely related to each other than either group is to \u2018<jats:italic>B. pinnipediae<\/jats:italic>\u2019 isolates. The groups identified by VNTR typing and MLSA are completely congruent. The relevance of these findings to current proposals to recognize two species of marine mammal <jats:italic>Brucella<\/jats:italic> is discussed.<\/jats:p>","DOI":"10.1099\/jmm.0.47330-0","type":"journal-article","created":{"date-parts":[[2007,10,26]],"date-time":"2007-10-26T18:24:52Z","timestamp":1193423092000},"page":"1512-1518","source":"Crossref","is-referenced-by-count":53,"title":["Molecular typing divides marine mammal strains of Brucella into at least three groups with distinct host preferences"],"prefix":"10.1099","volume":"56","author":[{"given":"Pauline","family":"Groussaud","sequence":"first","affiliation":[{"name":"Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency (VLA), New Haw, Addlestone, Surrey KT15 3NB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen J.","family":"Shankster","sequence":"additional","affiliation":[{"name":"Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency (VLA), New Haw, Addlestone, Surrey KT15 3NB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark S.","family":"Koylass","sequence":"additional","affiliation":[{"name":"Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency (VLA), New Haw, Addlestone, Surrey KT15 3NB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian M.","family":"Whatmore","sequence":"additional","affiliation":[{"name":"Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency (VLA), New Haw, Addlestone, Surrey KT15 3NB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/S1369-5274(00)00165-X","article-title":"Brucellosis: a worldwide zoonosis","volume":"4","author":"Boschiroli","year":"2001","journal-title":"Curr Opin Microbiol"},{"key":"R2","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetmic.2007.06.002","article-title":"The genomic structure of Brucella strains isolated from marine mammals gives clues to evolutionary history within the genus","author":"Bourg","year":"2007","journal-title":"Vet Microbiol"},{"key":"R3","first-page":"483","article-title":"Human exposure to Brucella recovered from a sea mammal","volume":"144","author":"Brew","year":"1999","journal-title":"Vet Rec"},{"key":"R4","doi-asserted-by":"crossref","first-page":"3267","DOI":"10.1099\/00221287-144-12-3267","article-title":"Phenotypic and molecular characterization of a Brucella strain isolated from a minke whale ( Balaenoptera acutorostrata","volume":"144","author":"Clavareau","year":"1998","journal-title":"Microbiology"},{"key":"R5","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1016\/S1286-4579(01)01427-7","article-title":"Classification of Brucella spp. isolated from marine mammals by DNA polymorphism at the omp2 locus","volume":"3","author":"Cloeckaert","year":"2001","journal-title":"Microbes Infect"},{"key":"R6","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1016\/S1286-4579(03)00091-1","article-title":"Classification of Brucella strains isolated from marine mammals by infrequent restriction site-PCR and development of specific PCR identification tests","volume":"5","author":"Cloeckaert","year":"2003","journal-title":"Microbes Infect"},{"key":"R7","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1007\/0-387-29298-5_90","article-title":"Genus I. 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