{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T21:24:20Z","timestamp":1789939460051,"version":"4.0.1"},"reference-count":37,"publisher":"Microbiology Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,3,1]]},"abstract":"\n Yersinia pestis<\/jats:italic>, the aetiological agent of the plague, causes sporadic disease in endemic areas of the world and is classified as a National Institute of Allergy and Infectious Diseases Category A Priority Pathogen because of its potential to be used as a bioweapon. Health departments, hospitals and government agencies need the ability to rapidly identify and characterize cultured isolates of this bacterium. Assays have been developed to perform this function; however, they are limited in their ability to distinguish Y. pestis<\/jats:italic> from Yersinia pseudotuberculosis<\/jats:italic>. This report describes the creation of a real-time PCR assay using Taqman probes that exclusively identifies Y. pestis<\/jats:italic> using a unique target sequence of the yihN<\/jats:italic> gene on the chromosome. As with other Y. pestis<\/jats:italic> PCR assays, three major genes located on each of the three virulence plasmids were included: lcrV<\/jats:italic> on pCD1, caf1<\/jats:italic> on pMT1 and pla<\/jats:italic> on pPCP1. The quadruplex assay was validated on a collection of 192 Y. pestis<\/jats:italic> isolates and 52 near-neighbour isolates. It was discovered that only 72\u200a% of natural plague isolates from the states of New Mexico and Utah harboured all three virulence plasmids. This quadruplex assay proved to be 100\u200a% successful in differentiating Y. pestis<\/jats:italic> from all near neighbours tested and was able to reveal which of the three virulence plasmids a particular isolate possessed.<\/jats:p>","DOI":"10.1099\/jmm.0.47485-0","type":"journal-article","created":{"date-parts":[[2008,2,20]],"date-time":"2008-02-20T17:08:55Z","timestamp":1203527335000},"page":"324-331","source":"Crossref","is-referenced-by-count":54,"title":["A quadruplex real-time PCR assay for the detection of Yersinia pestis and its plasmids"],"prefix":"10.1099","volume":"57","author":[{"given":"Alvin","family":"Stewart","sequence":"first","affiliation":[{"name":"851 WIDB, Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT 84602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Satterfield","sequence":"additional","affiliation":[{"name":"851 WIDB, Department of Microbiology and Molecular Biology, Brigham 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