{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T15:59:10Z","timestamp":1784735950567,"version":"3.55.0"},"reference-count":24,"publisher":"Microbiology Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,3,1]]},"abstract":"<jats:p>The aim of this study was to compare conventional 16S rRNA gene PCR, real-time 16S rRNA gene PCR and real-time <jats:italic>Mycoplasma genitalium<\/jats:italic> adhesin protein (MgPa) gene PCR as detection methods for <jats:italic>M. genitalium<\/jats:italic> infection. The study also determined the prevalence of <jats:italic>M. genitalium<\/jats:italic> in male and female patients attending a sexually transmitted infections clinic in a rural area in the west of Sweden. First void urine (FVU) and\/or urethral swabs were collected from 381 men, and FVU and\/or cervical swabs and\/or urethral swabs were collected from 298 women. A total of 213 specimens were used in the PCR comparative study: 98 consecutively sampled specimens from patients enrolled in the prevalence study, 36 consecutively sampled specimens from patients with symptoms of urethritis and 79 specimens from patients positive for <jats:italic>M. genitalium<\/jats:italic> by real-time MgPa gene PCR in the prevalence study. A true-positive <jats:italic>M. genitalium<\/jats:italic> DNA specimen was defined as either a specimen positive in any two PCR assays or a specimen whose PCR product was verified by DNA sequencing. The prevalence of <jats:italic>M. genitalium<\/jats:italic> infection in men and women was 27\/381 (7.1\u200a%) and 23\/298 (7.7\u200a%), respectively. In the PCR comparative study, <jats:italic>M. genitalium<\/jats:italic> DNA was detected in 61\/76 (80.3\u200a%) of true-positive specimens by conventional 16S rRNA gene PCR, in 52\/76 (68.4\u200a%) by real-time 16S rRNA gene PCR and in 74\/76 (97.4\u200a%) by real-time MgPa gene PCR. Real-time MgPa gene PCR thus had higher sensitivity compared with conventional 16S rRNA gene PCR and had considerably increased sensitivity compared with real-time 16S rRNA gene PCR for detection of <jats:italic>M. genitalium<\/jats:italic> DNA. Real-time MgPa gene PCR is well suited for the clinical diagnosis of <jats:italic>M. genitalium<\/jats:italic>.<\/jats:p>","DOI":"10.1099\/jmm.0.47498-0","type":"journal-article","created":{"date-parts":[[2008,2,20]],"date-time":"2008-02-20T17:08:55Z","timestamp":1203527335000},"page":"304-309","source":"Crossref","is-referenced-by-count":37,"title":["A comparative study of three different PCR assays for detection of Mycoplasma genitalium in urogenital specimens from men and women"],"prefix":"10.1099","volume":"57","author":[{"given":"Andreas","family":"Edberg","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology, Central Hospital, SE-651 85 Karlstad, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaretha","family":"Jurstrand","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, University Hospital, SE-701 85 \u00d6rebro, 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