{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T23:29:00Z","timestamp":1792193340195,"version":"4.4.0"},"reference-count":20,"publisher":"Microbiology Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,3,1]]},"abstract":"<jats:p>The chromogenic agar medium chromID ESBL (bioM\u00e9rieux) was compared with BLSE agar medium (AES) for selective isolation and presumptive identification of extended-spectrum <jats:italic>\u03b2<\/jats:italic>-lactamase (ESBL)-producing <jats:italic>Enterobacteriaceae<\/jats:italic> from clinical samples. A total of 765 samples (468 rectal swabs, 255 urine samples and 42 pulmonary aspirations) obtained from 547 patients was processed. All bacterial strains isolated on either medium were further characterized using biochemical tests, and ESBL producers were confirmed by synergy testing. Genetic characterization of ESBL genes was determined by PCR. A total of 33 ESBL-producing <jats:italic>Enterobacteriaceae<\/jats:italic> strains [<jats:italic>Escherichia coli<\/jats:italic> (<jats:italic>n<\/jats:italic>=16), <jats:italic>Klebsiella pneumoniae<\/jats:italic> (<jats:italic>n<\/jats:italic>=8), <jats:italic>Enterobacter<\/jats:italic> spp. (<jats:italic>n<\/jats:italic>=3), <jats:italic>Citrobacter<\/jats:italic> spp. (<jats:italic>n<\/jats:italic>=5) and <jats:italic>Proteus mirabilis<\/jats:italic> (<jats:italic>n<\/jats:italic>=1)] was recovered. The sensitivity after 24\u2005h incubation was 88\u200a% for chromID ESBL and 85\u200a% for BLSE agar. At 48\u2005h, the sensitivity of chromID ESBL increased to 94\u200a% and was higher than that obtained with BLSE agar. The positive predictive value at 24\u2005h for chromID ESBL was 38.7\u200a% [95\u200a% confidence interval (95\u200a% CI) 28.3\u200a\u201350.2\u200a%)], which was significantly higher than that for BLSE agar [15.4\u200a%, 95\u200a% CI 10.1\u200a\u201321.5\u200a%]. On both media, false-positive results were mostly due to <jats:italic>Pseudomonas aeruginosa<\/jats:italic> and to <jats:italic>Enterobacteriaceae<\/jats:italic> overproducing chromosomal cephalosporinase (<jats:italic>Enterobacter<\/jats:italic> spp.) or a chromosomal penicillinase (<jats:italic>Klebsiella oxytoca<\/jats:italic>). This study showed that chromID ESBL, a ready-to-use chromogenic selective medium, is sensitive and specific for rapid, presumptive identification of ESBL-producing <jats:italic>Enterobacteriaceae<\/jats:italic>. Its chromogenic properties and its selectivity are particularly useful in specimens containing resident associated flora.<\/jats:p>","DOI":"10.1099\/jmm.0.47625-0","type":"journal-article","created":{"date-parts":[[2008,2,20]],"date-time":"2008-02-20T17:08:55Z","timestamp":1203527335000},"page":"310-315","source":"Crossref","is-referenced-by-count":75,"title":["Performance of chromID ESBL, a chromogenic medium for detection of Enterobacteriaceae producing extended-spectrum \u03b2-lactamases"],"prefix":"10.1099","volume":"57","author":[{"given":"H\u00e9l\u00e8ne","family":"R\u00e9glier-Poupet","sequence":"first","affiliation":[{"name":"Service de Bact\u00e9riologie, H\u00f4pital Cochin, Assistance Publique H\u00f4pitaux de Paris, Facult\u00e9 de M\u00e9decine Paris Descartes, 27 Rue du Faubourg Saint Jacques, 75014 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Naas","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie-Virologie, INSERM U914: Emerging Resistance to Antibiotics, H\u00f4pital de Bic\u00eatre, Assistance Publique\/H\u00f4pitaux de Paris, Facult\u00e9 de M\u00e9decine Paris-Sud, Universit\u00e9 Paris XI, 78 Rue du G\u00e9n\u00e9ral Leclerc, 94275 Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Am\u00e9lie","family":"Carrer","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie-Virologie, INSERM U914: Emerging Resistance to Antibiotics, H\u00f4pital de Bic\u00eatre, Assistance Publique\/H\u00f4pitaux de Paris, Facult\u00e9 de M\u00e9decine Paris-Sud, Universit\u00e9 Paris XI, 78 Rue du G\u00e9n\u00e9ral Leclerc, 94275 Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Cady","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie, H\u00f4pital Cochin, Assistance Publique H\u00f4pitaux de Paris, Facult\u00e9 de M\u00e9decine Paris Descartes, 27 Rue du Faubourg Saint Jacques, 75014 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Marie","family":"Adam","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie, H\u00f4pital Cochin, Assistance Publique H\u00f4pitaux de Paris, Facult\u00e9 de M\u00e9decine Paris Descartes, 27 Rue du Faubourg Saint Jacques, 75014 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Fortineau","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie-Virologie, INSERM U914: Emerging Resistance to Antibiotics, H\u00f4pital de Bic\u00eatre, Assistance Publique\/H\u00f4pitaux de Paris, Facult\u00e9 de M\u00e9decine Paris-Sud, Universit\u00e9 Paris XI, 78 Rue du G\u00e9n\u00e9ral Leclerc, 94275 Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Poyart","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie, H\u00f4pital Cochin, Assistance Publique H\u00f4pitaux de Paris, Facult\u00e9 de M\u00e9decine Paris Descartes, 27 Rue du Faubourg Saint Jacques, 75014 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Nordmann","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie-Virologie, INSERM U914: Emerging Resistance to Antibiotics, H\u00f4pital de Bic\u00eatre, Assistance Publique\/H\u00f4pitaux de Paris, Facult\u00e9 de M\u00e9decine Paris-Sud, Universit\u00e9 Paris XI, 78 Rue du G\u00e9n\u00e9ral Leclerc, 94275 Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"3061","DOI":"10.1128\/AAC.44.11.3061-3068.2000","article-title":"A novel class A extended-spectrum \u03b2 -lactamase (BES-1) in Serratia marcescens isolated in Brazil","volume":"44","author":"Bonnet","year":"2000","journal-title":"Antimicrob Agents Chemother"},{"key":"R2","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1016\/j.mib.2006.08.011","article-title":"The CTX-M \u03b2 -lactamase pandemic","volume":"9","author":"Canton","year":"2006","journal-title":"Curr Opin Microbiol"},{"key":"R3","volume-title":"Performance standards for antimicrobial susceptibility testing , 15th informational supplement, M100-S15","year":"2005"},{"key":"R4","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1128\/JCM.44.2.586-588.2006","article-title":"Performance of MRSA ID, a new chromogenic medium for detection of methicillin-resistant Staphylococcus aureus","volume":"44","author":"Diederen","year":"2006","journal-title":"J Clin Microbiol"},{"key":"R5","doi-asserted-by":"crossref","first-page":"4767","DOI":"10.1128\/AAC.49.11.4767-4770.2005","article-title":"TLA-2, a novel Ambler class A expanded-spectrum \u03b2 -lactamase","volume":"49","author":"Girlich","year":"2005","journal-title":"Antimicrob Agents Chemother"},{"key":"R6","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1128\/JCM.02221-06","article-title":"Evaluation of a new selective chromogenic agar medium for detection of extended-spectrum \u03b2 -lactamase-producing Enterobacteriaceae","volume":"45","author":"Glupczynski","year":"2007","journal-title":"J Clin Microbiol"},{"key":"R7","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1093\/clinids\/10.4.867","article-title":"Extended broad-spectrum \u03b2 -lactamases conferring transferable resistance to newer \u03b2 -lactam agents in Enterobacteriaceae : hospital prevalence and susceptibility patterns","volume":"10","author":"Jarlier","year":"1988","journal-title":"Rev Infect Dis"},{"key":"R8","doi-asserted-by":"crossref","first-page":"1556","DOI":"10.1128\/JCM.02116-06","article-title":"Evaluation of a novel chromogenic agar medium for isolation and differentiation of vancomycin-resistant Enterococcus faecium and Enterococcus faecalis","volume":"45","author":"Ledeboer","year":"2007","journal-title":"J Clin Microbiol"},{"key":"R9","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1086\/313511","article-title":"Control of a prolonged outbreak of extended-spectrum \u03b2 -lactamase-producing Enterobacteriaceae in a university hospital","volume":"29","author":"Lucet","year":"1999","journal-title":"Clin Infect Dis"},{"key":"R10","doi-asserted-by":"crossref","first-page":"1745","DOI":"10.1128\/AAC.50.5.1745-1752.2006","article-title":"Complex genetic structures with repeated elements, a sul -type class 1 integron, and the bla VEB extended-spectrum \u03b2 -lactamase gene","volume":"50","author":"Naas","year":"2006","journal-title":"Antimicrob Agents Chemother"},{"key":"R11","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1128\/AAC.00611-06","article-title":"Identification of CTX-M-type extended-spectrum- \u03b2 -lactamase genes using real-time PCR and pyrosequencing","volume":"51","author":"Naas","year":"2007","journal-title":"Antimicrob Agents Chemother"},{"key":"R12","doi-asserted-by":"crossref","first-page":"S20","DOI":"10.1016\/j.amjmed.2006.03.013","article-title":"Resistance in Gram-negative bacteria: Enterobacteriaceae","volume":"119","author":"Paterson","year":"2006","journal-title":"Am J Med"},{"key":"R13","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0195-6701(97)90163-8","article-title":"Risk factors for faecal carriage of Klebsiella pneumoniae producing extended spectrum \u03b2 -lactamase (ESBL-KP) in the intensive care unit","volume":"35","author":"Pena","year":"1997","journal-title":"J Hosp Infect"},{"key":"R14","doi-asserted-by":"crossref","first-page":"S153","DOI":"10.1086\/500662","article-title":"Overview of the epidemiological profile and laboratory detection of extended-spectrum \u03b2 -lactamases","volume":"42","author":"Pfaller","year":"2006","journal-title":"Clin Infect Dis"},{"key":"R15","doi-asserted-by":"crossref","first-page":"2188","DOI":"10.1128\/AAC.41.10.2188","article-title":"OXA-18, a class D clavulanic acid-inhibited extended-spectrum \u03b2 -lactamase from Pseudomonas aeruginosa","volume":"41","author":"Philippon","year":"1997","journal-title":"Antimicrob Agents Chemother"},{"key":"R16","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1093\/jac\/dki166","article-title":"Emergence of Enterobacteriaceae producing extended-spectrum \u03b2 -lactamases (ESBLs) in the community","volume":"56","author":"Pitout","year":"2005","journal-title":"J Antimicrob Chemother"},{"key":"R17","doi-asserted-by":"crossref","first-page":"3743","DOI":"10.1128\/AAC.49.9.3743-3748.2005","article-title":"BEL-1, a novel clavulanic acid-inhibited extended-spectrum \u03b2 -lactamase, and the class 1 integron In120 in Pseudomonas aeruginosa","volume":"49","author":"Poirel","year":"2005","journal-title":"Antimicrob Agents Chemother"},{"key":"R18","doi-asserted-by":"crossref","first-page":"S164","DOI":"10.1086\/500663","article-title":"Extended-spectrum \u03b2 -lactamases and clinical outcomes: current data","volume":"42","author":"Ramphal","year":"2006","journal-title":"Clin Infect Dis"},{"key":"R19","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1128\/AAC.44.4.997-1003.2000","article-title":"TLA-1: a new plasmid-mediated extended-spectrum \u03b2 -lactamase from Escherichia coli","volume":"44","author":"Silva","year":"2000","journal-title":"Antimicrob Agents Chemother"},{"key":"R20","doi-asserted-by":"crossref","first-page":"4769","DOI":"10.1128\/JCM.42.10.4769-4775.2004","article-title":"Dramatic increase in prevalence of fecal carriage of extended-spectrum \u03b2 -lactamase-producing Enterobacteriaceae during nonoutbreak situations in Spain","volume":"42","author":"Valverde","year":"2004","journal-title":"J Clin Microbiol"}],"container-title":["Journal of Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.47625-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,28]],"date-time":"2020-09-28T05:17:59Z","timestamp":1601270279000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmm\/10.1099\/jmm.0.47625-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,3,1]]},"references-count":20,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2008,3,1]]}},"URL":"https:\/\/doi.org\/10.1099\/jmm.0.47625-0","relation":{},"ISSN":["0022-2615","1473-5644"],"issn-type":[{"value":"0022-2615","type":"print"},{"value":"1473-5644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,3,1]]}}}