{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T00:04:54Z","timestamp":1759104294774},"reference-count":43,"publisher":"Microbiology Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,3,1]]},"abstract":"<jats:p>\n                  <jats:italic>Bacillus anthracis<\/jats:italic>, the aetiological agent of anthrax, has been taxonomically classified with the <jats:italic>Bacillus cereus<\/jats:italic> group, which comprises <jats:italic>B. cereus<\/jats:italic>, <jats:italic>Bacillus thuringiensis<\/jats:italic>, <jats:italic>Bacillus mycoides<\/jats:italic>, <jats:italic>Bacillus pseudomycoides<\/jats:italic> and <jats:italic>Bacillus weihenstephanensis<\/jats:italic>. Although the pathogenesis and ecological manifestations may be different, <jats:italic>B. anthracis<\/jats:italic> shares a high degree of DNA sequence similarity with its group member species. As a result, the discrimination of <jats:italic>B. anthracis<\/jats:italic> from its close relatives in the <jats:italic>B. cereus<\/jats:italic> group is still quite difficult. Suppression subtractive hybridization (SSH) was performed to search for genomic differences between a <jats:italic>B. anthracis<\/jats:italic> Korean isolate CR and the most closely related <jats:italic>B. cereus<\/jats:italic> type strain KCTC 3624<jats:sup>T<\/jats:sup>. Two-hundred and five <jats:italic>B. anthracis<\/jats:italic> CR clones obtained by SSH underwent Southern hybridization, and comparative sequences were analysed using the <jats:sc>blast<\/jats:sc> program from the National Center for Biotechnology Information (NCBI). Subsequently, primer sets based on the glycosyltransferase group 1 family protein gene specific to <jats:italic>B. anthracis<\/jats:italic> were designed from the sequences of subtracted clones, and their specificities were evaluated using eight <jats:italic>B. anthracis<\/jats:italic>, 33 <jats:italic>B. cereus<\/jats:italic>, 10 <jats:italic>B. thuringiensis<\/jats:italic>, six <jats:italic>B. mycoides<\/jats:italic>, one <jats:italic>B. pseudomycoides<\/jats:italic>, one <jats:italic>B. weihenstephanensis<\/jats:italic> and 19 strains from 11 other representative <jats:italic>Bacillus<\/jats:italic> species. PCR primers specific for the glycosyltransferase group 1 family protein gene did not amplify the desired products from any of the <jats:italic>Bacillus<\/jats:italic> strains under examination, except <jats:italic>B. anthracis<\/jats:italic> alone. These findings may be useful in the future development of efficient diagnostic tools for the rapid identification of <jats:italic>B. anthracis<\/jats:italic> from other members of the <jats:italic>B. cereus<\/jats:italic> group.<\/jats:p>","DOI":"10.1099\/jmm.0.47642-0","type":"journal-article","created":{"date-parts":[[2008,2,20]],"date-time":"2008-02-20T17:08:55Z","timestamp":1203527335000},"page":"279-286","source":"Crossref","is-referenced-by-count":13,"title":["Glycosyltransferase \u2013 a specific marker for the discrimination of Bacillus anthracis from the Bacillus cereus group"],"prefix":"10.1099","volume":"57","author":[{"given":"Wonyong","family":"Kim","sequence":"first","affiliation":[{"name":"Research Institute for Translational System Biomics, Chung-Ang University College of Medicine, Seoul, Republic of Korea"},{"name":"Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji-Yeon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sung-Lim","family":"Cho","sequence":"additional","affiliation":[{"name":"Research Institute for Translational System Biomics, Chung-Ang University College of Medicine, Seoul, Republic of Korea"},{"name":"Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sun-Woo","family":"Nam","sequence":"additional","affiliation":[{"name":"Korea Health Industry Development Institute, Seoul, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong-Wook","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Chung-Ang 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