{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:02:19Z","timestamp":1791176539591,"version":"4.1.0"},"reference-count":17,"publisher":"Microbiology Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,5,1]]},"abstract":"<jats:p>Leptospirosis is a zoonotic disease with global distribution, caused by spirochaetes of the genus <jats:italic>Leptospira<\/jats:italic>. Transmission of <jats:italic>Leptospira interrogans<\/jats:italic> serovar Icterohaemorrhagiae, the causative agent of Weil's disease, to humans usually results from exposure to the urine of infected, but mostly asymptomatic, rodents, either by direct contact or indirectly through contaminated soil or water. Although regarded as a re-emerging infectious disease, human leptospirosis is probably underdiagnosed due to its often unspecific clinical appearance and difficulties in culturing leptospires. Therefore, more rapid and specific diagnostic procedures are needed. Here we describe a novel real-time quantitative PCR system developed for the accurate and fast diagnosis of pathogenic <jats:italic>Leptospira<\/jats:italic> spp. Its usefulness in the management of a patient with rat bite-associated multiorgan failure is demonstrated.<\/jats:p>","DOI":"10.1099\/jmm.0.47677-0","type":"journal-article","created":{"date-parts":[[2008,4,24]],"date-time":"2008-04-24T15:33:33Z","timestamp":1209051213000},"page":"658-663","source":"Crossref","is-referenced-by-count":30,"title":["Severe course of rat bite-associated Weil's disease in a patient diagnosed with a new Leptospira-specific real-time quantitative LUX-PCR"],"prefix":"10.1099","volume":"57","author":[{"given":"Alexandra","family":"Roczek","sequence":"first","affiliation":[{"name":"Bavarian Health and Food Safety Authority (LGL), Veterin\u00e4rstra\u00dfe 2, 85764 Oberschlei\u00dfheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Forster","sequence":"additional","affiliation":[{"name":"Universit\u00e4tsklinikum Erlangen, Medizinische Klinik 4, Krankenhausstra\u00dfe 12, 91054 Erlangen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heribert","family":"Raschel","sequence":"additional","affiliation":[{"name":"Bavarian Health and Food Safety Authority (LGL), Veterin\u00e4rstra\u00dfe 2, 85764 Oberschlei\u00dfheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"H\u00f6rmansdorfer","sequence":"additional","affiliation":[{"name":"Bavarian Health and Food Safety Authority (LGL), Veterin\u00e4rstra\u00dfe 2, 85764 Oberschlei\u00dfheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karl-Heinz","family":"Bogner","sequence":"additional","affiliation":[{"name":"Bavarian Health and Food Safety Authority (LGL), Veterin\u00e4rstra\u00dfe 2, 85764 Oberschlei\u00dfheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Hafner-Marx","sequence":"additional","affiliation":[{"name":"Bavarian Health and Food Safety Authority (LGL), Veterin\u00e4rstra\u00dfe 2, 85764 Oberschlei\u00dfheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Lepper","sequence":"additional","affiliation":[{"name":"Bavarian Health and Food Safety Authority (LGL), Veterin\u00e4rstra\u00dfe 2, 85764 Oberschlei\u00dfheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerhard","family":"Dobler","sequence":"additional","affiliation":[{"name":"Institute of Microbiology of the Bundeswehr, Neuherbergstra\u00dfe 11, 80937 M\u00fcnchen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias","family":"B\u00fcttner","sequence":"additional","affiliation":[{"name":"Bavarian Health and Food Safety Authority (LGL), Veterin\u00e4rstra\u00dfe 2, 85764 Oberschlei\u00dfheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Sing","sequence":"additional","affiliation":[{"name":"Bavarian Health and Food Safety Authority (LGL), Veterin\u00e4rstra\u00dfe 2, 85764 Oberschlei\u00dfheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.ijid.2005.02.010","article-title":"Trends in leptospirosis epidemiology in France. 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