{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T14:20:54Z","timestamp":1774794054647,"version":"3.50.1"},"reference-count":34,"publisher":"Microbiology Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,5,1]]},"abstract":"\n The\n Helicobacter pylori<\/jats:italic>\n duodenal ulcer promoting (\n dupA<\/jats:italic>\n ) gene has been previously described as a risk marker for duodenal ulcer (DU) development and a protective factor against gastric cancer (GC). Recent studies which have assessed the application of\n dupA<\/jats:italic>\n in the prediction of clinical outcomes have been controversial. In the current study, the association of\n dupA<\/jats:italic>\n with the clinical outcomes and histopathological changes following\n H. pylori<\/jats:italic>\n infection was evaluated in Iranian patients. A total of 157\n H. pylori<\/jats:italic>\n -infected patients with DU (\n n<\/jats:italic>\n =30), gastric ulcer (\n n<\/jats:italic>\n =23), gastritis (\n n<\/jats:italic>\n =68) or GC (\n n<\/jats:italic>\n =36) were assessed. The presence of\n jhp0917<\/jats:italic>\n and\n jhp0918<\/jats:italic>\n genes was determined by gene specific PCR. Gastric histopathological changes were recorded according to the updated Sydney system. Seventy-eight (49.7\u200a%) and 71 (45.2\u200a%) of the 157 tested strains, respectively, were positive and negative for both genes. The remaining 8 (5.09\u200a%) of the 157 strains were\n jhp0917<\/jats:italic>\n -positive\/\n jhp0918<\/jats:italic>\n -negative. Univariate analysis showed inverse associations between\n dupA<\/jats:italic>\n and histological features including dysplasia as the penultimate stage of GC and lymphoid follicles as a consequence of relatively long-standing\n H. pylori<\/jats:italic>\n -associated gastritis. The degrees of nucleotide sequence identity of Iranian strains to Colombian, Brazilian and Indian strains ranged from 86.1 to 100\u200a% for the aligned regions of\n jhp0917<\/jats:italic>\n , from 88\u200a to 98.8\u200a% for\n jhp0918<\/jats:italic>\n and from 93.4\u200a to 99.5\u200a% for the partial sequences of the\n dupA<\/jats:italic>\n gene. Despite the fact that possession of the\n dupA<\/jats:italic>\n gene showed no association with any disease category in our population as reported in several other countries, association of\n dupA<\/jats:italic>\n -negative strains of\n H. pylori<\/jats:italic>\n with pre-malignant lesions calls for additional studies to evaluate the role of this gene as a protective marker against GC.\n <\/jats:p>","DOI":"10.1099\/jmm.0.47776-0","type":"journal-article","created":{"date-parts":[[2008,4,24]],"date-time":"2008-04-24T11:33:33Z","timestamp":1209036813000},"page":"554-562","source":"Crossref","is-referenced-by-count":44,"title":["dupA as a risk determinant in Helicobacter pylori infection"],"prefix":"10.1099","volume":"57","author":[{"given":"Masoumeh","family":"Douraghi","sequence":"first","affiliation":[{"name":"Department of Pathobiology, School of Public Health, Medical Sciences\/University of Tehran, Tehran, Iran"},{"name":"Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran, 13164"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marjan","family":"Mohammadi","sequence":"additional","affiliation":[{"name":"Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran, 13164"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akbar","family":"Oghalaie","sequence":"additional","affiliation":[{"name":"Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran, 13164"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Afshin","family":"Abdirad","sequence":"additional","affiliation":[{"name":"Cancer Research Center, Medical Sciences\/University of Tehran, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Ali","family":"Mohagheghi","sequence":"additional","affiliation":[{"name":"Cancer Research Center, Medical Sciences\/University of Tehran, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahmoud Eshagh","family":"Hosseini","sequence":"additional","affiliation":[{"name":"Endoscopy Unit, Amiralam Hospital, Medical Sciences\/University of Tehran, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hojat","family":"Zeraati","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, School of Public Health, Medical Sciences\/University of Tehran, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amir","family":"Ghasemi","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, School of Public Health, Medical Sciences\/University of Tehran, Tehran, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryam","family":"Esmaieli","sequence":"additional","affiliation":[{"name":"Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran, 13164"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nazanin","family":"Mohajerani","sequence":"additional","affiliation":[{"name":"Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran, 13164"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1007\/s001099900067","article-title":"Analysis of the genetic diversity of Helicobacter pylori : the tale of two genomes","volume":"77","author":"Alm","year":"1999","journal-title":"J Mol Med"},{"key":"R2","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1038\/16495","article-title":"Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori","volume":"397","author":"Alm","year":"1999","journal-title":"Nature"},{"key":"R3","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1111\/j.1523-5378.2007.00557.x","article-title":"Prevalence of duodenal 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production","volume":"123","author":"Yamaoka","year":"2002","journal-title":"Gastroenterology"},{"key":"R33","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1136\/gut.51.4.480","article-title":"Relationship between Helicobacter pylori babA2 status with gastric epithelial cell turnover and premalignant gastric lesions","volume":"51","author":"Yu","year":"2002","journal-title":"Gut"},{"key":"R34","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1136\/jcp.56.4.287","article-title":"Helicobacter pylori babA2 , cagA , and s1 vacA genes work synergistically in causing intestinal metaplasia","volume":"56","author":"Zambon","year":"2003","journal-title":"J Clin Pathol"}],"container-title":["Journal of Medical 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