{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T01:39:28Z","timestamp":1773279568233,"version":"3.50.1"},"reference-count":17,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2017,10,16]],"date-time":"2017-10-16T00:00:00Z","timestamp":1508112000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,10,16]]},"abstract":"<jats:p>\n                  <jats:bold>Introduction.<\/jats:bold> Leptospirosis is a zoonotic bacterial disease of global distribution affecting humans and animals. The initial phase of leptospirosis resembles many other febrile illness and due to its broad and biphasic clinical manifestations, selection and implementation of appropriate diagnostic tests can be challenging.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Case presentation.<\/jats:bold> This report describes a case investigation of a 14\u2009weeks old male, orphan puppy, presented with generalised jaundice, anemia, weakness, and anorexia. Clinical abnormalities included the evidence of renal and hepatic failure. Antemortem and postmortem diagnostic investigations were conducted to identify the cause of illness. PCR testing and culture of blood was positive for <jats:italic>Leptospira<\/jats:italic> sp. Necropsy followed by histopathology evaluation revealed lesions compatible with liver and kidney damage consisting of marked diffuse hepatocellular dissociation, acute renal tubular necrosis, and mild interstitial nephritis.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion.<\/jats:bold> Multiple diagnostic techniques including bacterial isolation confirmed <jats:italic>Leptospira<\/jats:italic> infection in this puppy. Whole genome sequencing and analysis identified the <jats:italic>Leptospira<\/jats:italic> sp. isolated from this puppy as <jats:italic>Leptospira interrogans<\/jats:italic> serovar Copenhageni. To our knowledge, this case report describes the first isolation of <jats:italic>Leptospira<\/jats:italic> from Saint Kitts. This case highlights the usefulness of including multiple diagnostic tests for the diagnosis and epidemiological investigation of <jats:italic>Leptospira<\/jats:italic> infection. Accurate diagnosis followed by timely intervention can prevent case fatality and mortality in infected patients.<\/jats:p>","DOI":"10.1099\/jmmcr.0.005120","type":"journal-article","created":{"date-parts":[[2017,10,23]],"date-time":"2017-10-23T16:03:11Z","timestamp":1508774591000},"source":"Crossref","is-referenced-by-count":13,"title":["Isolation and characterization of Leptospira interrogans serovar Copenhageni from a dog from Saint Kitts"],"prefix":"10.1099","volume":"4","author":[{"given":"Christopher R.","family":"Larson","sequence":"first","affiliation":[{"name":"Ross University School of Veterinary Medicine, Basseterre, St. Kitts, West Indies"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle","family":"Dennis","sequence":"additional","affiliation":[{"name":"Ross University School of Veterinary Medicine, Basseterre, St. Kitts, West Indies"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajeev V.","family":"Nair","sequence":"additional","affiliation":[{"name":"Ross University School of Veterinary Medicine, Basseterre, St. Kitts, West Indies"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"Llanes","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Celular y Molecular de Enfermedades, Instituto de Investigaciones Cient\u00edficas y Servicios de Alta Tecnolog\u00eda (INDICASAT AIP), Ciudad del Saber, Panama"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Peda","sequence":"additional","affiliation":[{"name":"Ross University School of Veterinary Medicine, Basseterre, St. Kitts, West Indies"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shamara","family":"Welcome","sequence":"additional","affiliation":[{"name":"Ross University School of Veterinary Medicine, Basseterre, St. Kitts, West Indies"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sreekumari","family":"Rajeev","sequence":"additional","affiliation":[{"name":"Ross University School of Veterinary Medicine, Basseterre, St. Kitts, West Indies"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.2.296-326.2001"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-45059-8_6"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2010.0654.x"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1111\/jsap.12328"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2009.03.014"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0159387"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(97)00127-2"},{"key":"R8","first-page":"45","article-title":"Canine leptospirosis in Puerto Rico","volume":"9","author":"Farrington","year":"1982","journal-title":"Int J Zoonoses"},{"key":"R9","first-page":"138","article-title":"Pathogenic Leptospira isolates from the Caribbean Island of Barbados","volume":"9","author":"Jones","year":"1982","journal-title":"Int J Zoonoses"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268809990902"},{"key":"R11","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1016\/S0140-6736(99)80012-9","article-title":"Urban epidemic of severe leptospirosis in Brazil","volume":"354","author":"Ai","year":"1999","journal-title":"Lancet"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc12e05s32"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005025"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.4061\/2010\/928541"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.2460\/javma.243.9.1316"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.2460\/javma.240.8.957"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003898"}],"container-title":["JMM Case Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmmcr\/10.1099\/jmmcr.0.005120?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T11:29:09Z","timestamp":1702639749000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmmcr\/10.1099\/jmmcr.0.005120"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10,16]]},"references-count":17,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2017,10,16]]}},"URL":"https:\/\/doi.org\/10.1099\/jmmcr.0.005120","relation":{},"ISSN":["2053-3721"],"issn-type":[{"value":"2053-3721","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,10,16]]}}}