{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:50:19Z","timestamp":1787010619486,"version":"build-2736575974"},"reference-count":18,"publisher":"Microbiology Society","issue":"1","license":[{"start":{"date-parts":[[2018,1,2]],"date-time":"2018-01-02T00:00:00Z","timestamp":1514851200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,1,2]]},"abstract":"<jats:p>\n                    <jats:bold>Introduction.<\/jats:bold>\n                    Pythiosis is caused by\n                    <jats:italic>Pythium insidiosum<\/jats:italic>\n                    , a fungus-like organism in the class\n                    <jats:italic>Oomycetes<\/jats:italic>\n                    . It can infect humans and a variety of animal species in tropical, subtropical and some temperate regions. Cases of animal pythiosis have occurred predominantly in horses in the skin and subcutaneous tissue at the limbs and in the ventral portion of thoracoabdominal wall - lesions in the nasal region are rarely reported. Moreover, although many human pythiosis cases have been reported in Thailand, no cases of animal pythiosis in Thailand have been reported.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Case presentation.<\/jats:bold>\n                    We report a case of pythiosis in a horse infected at the nasal cavity. Diagnosis was performed by zoospore formation by bait technique, immunohistochemical stain, immunochromatography and sequence analysis.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion.<\/jats:bold>\n                    The sequences of rDNA were 99\u200a% and 96 to 99\u200a% identical to GenBank isolates of\n                    <jats:italic>Pythium insidiosum<\/jats:italic>\n                    from two Thai human patients and horses from various countries, respectively. This represents the first confirmed report of nasal equine pythiosis in Thailand.\n                  <\/jats:p>","DOI":"10.1099\/jmmcr.0.005136","type":"journal-article","created":{"date-parts":[[2018,1,9]],"date-time":"2018-01-09T09:40:43Z","timestamp":1515490843000},"source":"Crossref","is-referenced-by-count":10,"title":["First confirmed case of nasal pythiosis in a horse in Thailand"],"prefix":"10.1099","volume":"5","author":[{"given":"Walaiporn","family":"Tonpitak","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Veterinary Medicine, Mahanakorn University of Technology, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Watcharapol","family":"Pathomsakulwong","sequence":"additional","affiliation":[{"name":"Equine Clinic, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen campus, Nakhon Pathom, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chulabha","family":"Sornklien","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Veterinary Medicine, Mahanakorn University of Technology, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theerapong","family":"Krajaejun","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suppathat","family":"Wutthiwithayaphong","sequence":"additional","affiliation":[{"name":"Clinic for Horse, Faculty of Veterinary Medicine, Mahanakorn University of Technology, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2010.07.019"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1080\/13693780701513840"},{"key":"R3","first-page":"623","article-title":"Pythium insidiosum Thai isolates: molecular phylogenetic analysis","volume":"6","author":"Chaiprasert","year":"2009","journal-title":"Asian Biomedicine"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1086\/506353"},{"key":"R5","first-page":"151","article-title":"Infection caused by the oomycetous pathogen Pythium insidiosum","volume":"6","author":"Mendoza","year":"1996","journal-title":"J Mycol Med"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2016.06.005"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2013.02.025"},{"key":"R8","first-page":"1298","article-title":"Evaluation of an in-house immunoperoxidase staining assay for histodiagnosis of human pythiosis","volume":"40","author":"Keeratijarut","year":"2009","journal-title":"Southeast Asian J Trop Med Public Health"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1007\/BF00437925"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myw018"},{"key":"R11","first-page":"342","article-title":"Efficiency comparison of three methods for extracting genomic DNA of the pathogenic oomycete Pythium insidiosum","volume":"97","author":"Lohnoo","year":"2014","journal-title":"J Med Assoc Thai"},{"key":"R12","first-page":"315","article-title":"Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics","volume-title":"PCR Prortocols: a Guide to Methods and Applications","author":"White","year":"1990"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2017.08.004"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-015-0472-z"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1136\/vr.163.9.276"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2012.12.002"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1177\/104063870201400403"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-012-9562-7"}],"container-title":["JMM Case Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmmcr\/10.1099\/jmmcr.0.005136?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T07:32:10Z","timestamp":1702625530000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/jmmcr\/10.1099\/jmmcr.0.005136"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1,2]]},"references-count":18,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,1,2]]}},"URL":"https:\/\/doi.org\/10.1099\/jmmcr.0.005136","relation":{},"ISSN":["2053-3721"],"issn-type":[{"value":"2053-3721","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,1,2]]}}}