{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T17:52:34Z","timestamp":1776016354454,"version":"3.50.1"},"reference-count":32,"publisher":"Microbiology Society","issue":"2","license":[{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,2,1]]},"abstract":"<jats:p>\n                  <jats:italic>Aspergillus fumigatus<\/jats:italic> is an opportunistic, ubiquitous, saprophytic mould that can cause severe allergic responses in atopic individuals as well as life-threatening infections in immunocompromised patients. A critical step in the establishment of infection is the invasion of airway epithelial cells by the inhaled fungi. Understanding how <jats:italic>A. fumigatus<\/jats:italic> senses and responds to airway cells is important to understand the pathogenesis of invasive pulmonary aspergillosis. Here, we analysed the transcriptomes of two commonly used clinical isolates, Af293 and CEA10, during infection of the A549 type II pneumocyte cell line <jats:italic>in vitro<\/jats:italic>. We focused our RNA-seq analysis on the core set of genes that are present in the genomes of the two strains. Our results suggest that: (a) <jats:italic>A. fumigatus<\/jats:italic> does not mount a conserved transcriptional response to airway epithelial cells in our <jats:italic>in vitro<\/jats:italic> model and (b) strain background and time spent in the tissue culture media have a greater impact on the transcriptome than the presence of host cells. Our analyses reveal both common and strain-specific transcriptional programmes that allow for the generation of hypotheses about gene function as it pertains to pathogenesis and the significant phenotypic heterogeneity that is observed among <jats:italic>A. fumigatus<\/jats:italic> isolates.<\/jats:p>","DOI":"10.1099\/mgen.0.000154","type":"journal-article","created":{"date-parts":[[2018,1,18]],"date-time":"2018-01-18T13:13:29Z","timestamp":1516281209000},"source":"Crossref","is-referenced-by-count":19,"title":["Comparative transcriptomics of Aspergillus fumigatus strains upon exposure to human airway epithelial cells"],"prefix":"10.1099","volume":"4","author":[{"given":"Tonya N.","family":"Watkins","sequence":"first","affiliation":[{"name":"1\u200bInstitute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Liu","sequence":"additional","affiliation":[{"name":"2\u200bDivision of Infectious Diseases, Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew","family":"Chung","sequence":"additional","affiliation":[{"name":"1\u200bInstitute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tracy H.","family":"Hazen","sequence":"additional","affiliation":[{"name":"1\u200bInstitute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie C.","family":"Dunning Hotopp","sequence":"additional","affiliation":[{"name":"1\u200bInstitute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott G.","family":"Filler","sequence":"additional","affiliation":[{"name":"2\u200bDivision of Infectious Diseases, Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, CA, USA"},{"name":"3\u200bDavid Geffen School of Medicine at UCLA, Los Angeles, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent M.","family":"Bruno","sequence":"additional","affiliation":[{"name":"1\u200bInstitute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020527"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2012.00346"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004413"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-11-358"},{"key":"R5","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1097\/00005792-200007000-00006","article-title":"Invasive aspergillosis. 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