{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T21:57:07Z","timestamp":1791583027896,"version":"4.3.4"},"reference-count":30,"publisher":"Microbiology Society","issue":"5","license":[{"start":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T00:00:00Z","timestamp":1556668800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,5,1]]},"abstract":"We examined whether genomic surveillance of \n \n Escherichia coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> in wastewater could capture the dominant \n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> lineages associated with bloodstream infection and livestock in the East of England, together with the antibiotic-resistance genes circulating in the wider \n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> population. Treated and untreated wastewater was taken from 20 municipal treatment plants in the East of England, half in direct receipt of acute hospital waste. All samples were culture positive for \n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, and all but one were positive for extended-spectrum \u03b2-lactamase (ESBL)-producing \n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. The most stringent wastewater treatment (tertiary including UV light) did not eradicate ESBL-\n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> in 2\/3 cases. We sequenced 388 \n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> (192 ESBL, 196 non-ESBL). Multilocus sequence type (ST) diversity was similar between plants in direct receipt of hospital waste versus the remainder (93 vs 95 STs, respectively). We compared the genomes of wastewater \n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> with isolates from bloodstream infection (n<\/jats:italic>=437), and livestock farms and retail meat (n<\/jats:italic>=431) in the East of England. A total of 19\/20 wastewater plants contained one or more of the three most common STs associated with bloodstream infection (ST131, ST73, ST95), and 14\/20 contained the most common livestock ST (ST10). In an analysis of 1254 genomes (2 cryptic \n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> were excluded), wastewater isolates were distributed across the phylogeny and intermixed with isolates from humans and livestock. Ten bla<\/jats:italic>\n CTX-M<\/jats:sub> elements were identified in \n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> isolated from wastewater, together with a further 47 genes encoding resistance to the major antibiotic drug groups. Genes encoding resistance to colistin and the carbapenems were not detected. Genomic surveillance of \n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> in wastewater could be used to monitor new and circulating lineages and resistance determinants of public-health importance.<\/jats:p>","DOI":"10.1099\/mgen.0.000267","type":"journal-article","created":{"date-parts":[[2019,5,20]],"date-time":"2019-05-20T15:13:17Z","timestamp":1558365197000},"source":"Crossref","is-referenced-by-count":36,"title":["Genomic surveillance of Escherichia coli in municipal wastewater treatment plants as an indicator of clinically relevant pathogens and their resistance genes"],"prefix":"10.1099","volume":"5","author":[{"given":"Kathy E.","family":"Raven","sequence":"first","affiliation":[{"name":"1 Department of Medicine, University of Cambridge, Box 157 Addenbrooke\u2019s Hospital, Hills Road, Cambridge CB2 0QQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Ludden","sequence":"additional","affiliation":[{"name":"2 London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theodore","family":"Gouliouris","sequence":"additional","affiliation":[{"name":"1 Department of Medicine, University of Cambridge, Box 157 Addenbrooke\u2019s Hospital, Hills Road, Cambridge CB2 0QQ, UK"},{"name":"3 Clinical Microbiology and Public Health Laboratory, Public Health England, Cambridge CB2 0QQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beth","family":"Blane","sequence":"additional","affiliation":[{"name":"1 Department of Medicine, University of Cambridge, Box 157 Addenbrooke\u2019s Hospital, Hills Road, Cambridge CB2 0QQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Plamena","family":"Naydenova","sequence":"additional","affiliation":[{"name":"1 Department of Medicine, University of Cambridge, Box 157 Addenbrooke\u2019s Hospital, Hills Road, Cambridge CB2 0QQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas M.","family":"Brown","sequence":"additional","affiliation":[{"name":"3 Clinical Microbiology and Public Health Laboratory, Public Health England, Cambridge CB2 0QQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7069-5958","authenticated-orcid":false,"given":"Julian","family":"Parkhill","sequence":"additional","affiliation":[{"name":"4 Wellcome Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1718-2782","authenticated-orcid":false,"given":"Sharon J.","family":"Peacock","sequence":"additional","affiliation":[{"name":"4 Wellcome Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK"},{"name":"1 Department of Medicine, University of Cambridge, Box 157 Addenbrooke\u2019s Hospital, Hills Road, Cambridge CB2 0QQ, 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