{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:10:13Z","timestamp":1747181413785,"version":"3.40.5"},"reference-count":36,"publisher":"Microbiology Society","issue":"1","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Don Carlo Gnocchi Foundation","award":["project N. 1345"],"award-info":[{"award-number":["project N. 1345"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,1,1]]},"abstract":"<jats:p>In this work, we used a whole-genome sequencing (WGS) approach to study the features of KPC-producing <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.10789\" xlink:type=\"simple\">Klebsiella pneumoniae<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> (KPC-Kp) spreading in a large Italian long-term acute-care rehabilitation facility (LTACRF), and to track the dynamics of dissemination within this setting. Thirty-eight, non-replicated, KPC-Kp isolates from colonized patients (either already colonized at admission or colonized during admission), collected during 2016, were subjected to antimicrobial-susceptibility testing and WGS. All isolates were resistant to \u03b2-lactams, with the exception of ceftazidime\/avibactam (97.4\u200a% susceptible). The second most effective agent was fosfomycin, followed by colistin, trimethoprim\/sulfamethoxazole, gentamicin and amikacin (92.1, 86.8, 60.5, 44.7 and 50\u200a% of susceptibility, respectively). A large proportion of isolates (<jats:italic>n<\/jats:italic>=18\/38, 47.4%) belonged to clonal group (CG) 101, and most of them (<jats:italic>n<\/jats:italic>=15) to a new sequence type (ST) designated as ST2502. All the CG101 isolates had a capsule locus type KL17. The ST2502 harboured the genes encoding for the yersiniabactin siderophore and the ArmA methylase, conferring high-level resistance to aminoglycosides. The second most represented lineage of isolates (16\/38, 42.1%) belonged to ST512 of CG258. Analysing WGS data, we were able to ascertain the common origin of some isolates imported from other hospitals, and to track several clusters of in-LTACRF cross-transmissions. The results revealed that, in peculiar epidemiological settings such as LTACRF, new KPC-Kp clones different from those prevailing in acute-care hospitals and associated with uncommon resistance and virulence determinants can successfully emerge and disseminate.<\/jats:p>","DOI":"10.1099\/mgen.0.000308","type":"journal-article","created":{"date-parts":[[2020,1,31]],"date-time":"2020-01-31T11:10:27Z","timestamp":1580469027000},"source":"Crossref","is-referenced-by-count":11,"title":["Population structure of KPC carbapenemase-producing Klebsiella pneumoniae in a long-term acute-care rehabilitation facility: identification of a new lineage of clonal group 101, associated with local hyperendemicity"],"prefix":"10.1099","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7265-3698","authenticated-orcid":false,"given":"Fabio","family":"Arena","sequence":"first","affiliation":[{"name":"Department of Medical Biotechnologies, University of Siena, Siena, Italy"},{"name":"Present address: Department of Clinical and Experimental Medicine, University of Foggia, Foggia, Italy"},{"name":"Present address: Don Carlo Gnocchi Foundation, Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5863-5805","authenticated-orcid":false,"given":"Vincenzo","family":"Di Pilato","sequence":"additional","affiliation":[{"name":"Present address: Department of Surgical Sciences and Integrated Diagnostics (DISC), University of Genoa, Genoa, Italy"},{"name":"Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9403-7944","authenticated-orcid":false,"given":"Federica","family":"Vannetti","sequence":"additional","affiliation":[{"name":"Don Carlo Gnocchi Foundation, Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Fabbri","sequence":"additional","affiliation":[{"name":"Don Carlo Gnocchi Foundation, Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0295-6877","authenticated-orcid":false,"given":"Alberto","family":"Antonelli","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Coppi","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Pupillo","sequence":"additional","affiliation":[{"name":"Don Carlo Gnocchi Foundation, Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudio","family":"Macchi","sequence":"additional","affiliation":[{"name":"Don Carlo Gnocchi Foundation, Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gian Maria","family":"Rossolini","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Virology Unit, Florence Careggi University Hospital, Florence, Italy"},{"name":"Department of Experimental and Clinical Medicine, University of Florence, Florence, 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