{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:49:33Z","timestamp":1787010573910,"version":"build-2736575974"},"reference-count":101,"publisher":"Microbiology Society","issue":"9","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y T\u00e9cnol\u00f3gica","award":["PICT-2015-1072"],"award-info":[{"award-number":["PICT-2015-1072"]}]},{"DOI":"10.13039\/501100003074","name":"ANPCyT","doi-asserted-by":"crossref","award":["PICT-2017-3536"],"award-info":[{"award-number":["PICT-2017-3536"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002923","name":"Conicet","doi-asserted-by":"crossref","award":["PIP2017-11220170100377CO"],"award-info":[{"award-number":["PIP2017-11220170100377CO"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,9,1]]},"abstract":"<jats:p>\n                    <jats:italic>\n                      <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.2767\" xlink:type=\"simple\">Acinetobacter baumannii<\/jats:ext-link>\n                      <\/jats:named-content>\n                    <\/jats:italic>\n                    (\n                    <jats:italic>Aba<\/jats:italic>\n                    ) is an emerging opportunistic pathogen associated to nosocomial infections. The rapid increase in multidrug resistance (MDR) among\n                    <jats:italic>Aba<\/jats:italic>\n                    strains underscores the urgency of understanding how this pathogen evolves in the clinical environment. We conducted here a whole-genome sequence comparative analysis of three phylogenetically and epidemiologically related MDR\n                    <jats:italic>Aba<\/jats:italic>\n                    strains from Argentinean hospitals, assigned to the CC104\n                    <jats:sup>O<\/jats:sup>\n                    \/CC15\n                    <jats:sup>P<\/jats:sup>\n                    clonal complex. While the Ab244 strain was carbapenem-susceptible, Ab242 and Ab825, isolated after the introduction of carbapenem therapy, displayed resistance to these last resource \u03b2-lactams. We found a high chromosomal synteny among the three strains, but significant differences at their accessory genomes. Most importantly, carbapenem resistance in Ab242 and Ab825 was attributed to the acquisition of a Rep_3 family plasmid carrying a\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>OXA-58<\/jats:sub>\n                    gene. Other differences involved a genomic island carrying resistance to toxic compounds and a Tn\n                    <jats:italic>10<\/jats:italic>\n                    element exclusive to Ab244 and Ab825, respectively. Also remarkably, 44 insertion sequences (ISs) were uncovered in Ab825, in contrast with the 14 and 11 detected in Ab242 and Ab244, respectively. Moreover, Ab825 showed a higher killing capacity as compared to the other two strains in the\n                    <jats:italic>Galleria mellonella<\/jats:italic>\n                    infection model. A search for virulence and persistence determinants indicated the loss or IS-mediated interruption of genes encoding many surface-exposed macromolecules in Ab825, suggesting that these events are responsible for its higher relative virulence. The comparative genomic analyses of the CC104\n                    <jats:sup>O<\/jats:sup>\n                    \/CC15\n                    <jats:sup>P<\/jats:sup>\n                    strains conducted here revealed the contribution of acquired mobile genetic elements such as ISs and plasmids to the adaptation of\n                    <jats:italic>\n                      <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.2767\" xlink:type=\"simple\">A. baumannii<\/jats:ext-link>\n                      <\/jats:named-content>\n                    <\/jats:italic>\n                    to the clinical setting.\n                  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