{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T02:37:43Z","timestamp":1777084663255,"version":"3.51.4"},"reference-count":76,"publisher":"Microbiology Society","issue":"5","license":[{"start":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T00:00:00Z","timestamp":1588291200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,5,1]]},"abstract":"<jats:p>This study sought to assess the genetic variability of <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3093\" xlink:type=\"simple\">Escherichia coli<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> isolated from bloodstream infections (BSIs) presenting at Concord Hospital, Sydney during 2013\u20132016. Whole-genome sequencing was used to characterize 81 <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3093\" xlink:type=\"simple\">E. coli<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> isolates sourced from community-onset (CO) and hospital-onset (HO) BSIs. The cohort comprised 64 CO and 17 HO isolates, including 35 multidrug-resistant (MDR) isolates exhibiting phenotypic resistance to three or more antibiotic classes. Phylogenetic analysis identified two major ancestral clades. One was genetically diverse with 25 isolates distributed in 16 different sequence types (STs) representing phylogroups A, B1, B2, C and F, while the other comprised phylogroup B2 isolates in subclades representing the ST131, ST73 and ST95 lineages. Forty-seven isolates contained a class 1 integron, of which 14 carried <jats:italic>bla<\/jats:italic>\n                  <jats:sub>CTX -M-<\/jats:sub>gene. Isolates with a class 1 integron carried more antibiotic resistance genes than isolates without an integron and, in most instances, resistance genes were localized within complex resistance loci (CRL). Resistance to fluoroquinolones could be attributed to point mutations in chromosomal <jats:italic>parC<\/jats:italic> and <jats:italic>gyrB<\/jats:italic> genes and, in addition, two isolates carried a plasmid-associated <jats:italic>qnrB4<\/jats:italic> gene. Co-resistance to fluoroquinolone and broad-spectrum beta-lactam antibiotics was associated with ST131 (HO and CO), ST38 (HO), ST393 (CO), ST2003 (CO) and ST8196 (CO and HO), a novel ST identified in this study. Notably, 10\/81 (12.3\u200a%) isolates with ST95 (5 isolates), ST131 (2 isolates), ST88 (2 isolates) and a ST540 likely carry IncFII\u2013IncFIB plasmid replicons with a full spectrum of virulence genes consistent with the carriage of ColV-like plasmids. Our data indicate that IncF plasmids play an important role in shaping virulence and resistance gene carriage in BSI <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3093\" xlink:type=\"simple\">E. coli<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> in Australia.<\/jats:p>","DOI":"10.1099\/mgen.0.000371","type":"journal-article","created":{"date-parts":[[2020,5,6]],"date-time":"2020-05-06T16:22:47Z","timestamp":1588782167000},"source":"Crossref","is-referenced-by-count":18,"title":["Genomic profiling of Escherichia coli isolates from bacteraemia patients: a 3-year cohort study of isolates collected at a Sydney teaching hospital"],"prefix":"10.1099","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4851-3855","authenticated-orcid":false,"given":"Priyanka","family":"Hastak","sequence":"first","affiliation":[{"name":"Australian Centre for Genomic Epidemiological Microbiology, University of Technology Sydney, PO Box 123, Broadway, NSW 2007, Australia"},{"name":"The ithree institute, University of Technology Sydney, City Campus, Ultimo, NSW 2007, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9333-7160","authenticated-orcid":false,"given":"Max L.","family":"Cummins","sequence":"additional","affiliation":[{"name":"The ithree institute, University of Technology Sydney, City Campus, Ultimo, NSW 2007, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Gottlieb","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Sydney, NSW Australia"},{"name":"Department of Microbiology and Infectious Diseases, Concord Hospital and NSW Health Pathology, Hospital Road, Concord 2139, NSW, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elaine","family":"Cheong","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, Concord Hospital and NSW Health Pathology, Hospital Road, Concord 2139, NSW, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Merlino","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Sydney, NSW Australia"},{"name":"Department of Microbiology and Infectious Diseases, Concord Hospital and NSW Health Pathology, Hospital Road, Concord 2139, NSW, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4756-4810","authenticated-orcid":false,"given":"Garry S. 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