{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:20:39Z","timestamp":1791249639189,"version":"4.1.0"},"reference-count":101,"publisher":"Microbiology Society","issue":"12","license":[{"start":{"date-parts":[[2021,12,24]],"date-time":"2021-12-24T00:00:00Z","timestamp":1640304000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["UH3AI083263"],"award-info":[{"award-number":["UH3AI083263"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000071","name":"National Institute of Child Health and Human Development","doi-asserted-by":"crossref","award":["U54HD080784; R01HD092415"],"award-info":[{"award-number":["U54HD080784; R01HD092415"]}],"id":[{"id":"10.13039\/100000071","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"crossref","award":["GAPPS PPB"],"award-info":[{"award-number":["GAPPS PPB"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","award":["R21HD092965"],"award-info":[{"award-number":["R21HD092965"]}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R25GM090084"],"award-info":[{"award-number":["R25GM090084"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,12,24]]},"abstract":"<jats:p>\n                    The composition of the human vaginal microbiome has been extensively studied and is known to influence reproductive health. However, the functional roles of individual taxa and their contributions to negative health outcomes have yet to be well characterized. Here, we examine two vaginal bacterial taxa grouped within the genus\n                    <jats:italic>\n                      <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.4398\" xlink:type=\"simple\">Megasphaera<\/jats:ext-link>\n                      <\/jats:named-content>\n                    <\/jats:italic>\n                    that have been previously associated with bacterial vaginosis (BV) and pregnancy complications. Phylogenetic analyses support the classification of these taxa as two distinct species. These two phylotypes,\n                    <jats:italic>\n                      <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.4398\" xlink:type=\"simple\">Megasphaera<\/jats:ext-link>\n                      <\/jats:named-content>\n                    <\/jats:italic>\n                    phylotype 1 (MP1) and\n                    <jats:italic>\n                      <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.4398\" xlink:type=\"simple\">Megasphaera<\/jats:ext-link>\n                      <\/jats:named-content>\n                    <\/jats:italic>\n                    phylotype 2 (MP2), differ in genomic structure and metabolic potential, suggestive of differential roles within the vaginal environment. Further, these vaginal taxa show evidence of genome reduction and changes in DNA base composition, which may be common features of host dependence and\/or adaptation to the vaginal environment. In a cohort of 3870\u2009women, we observed that MP1 has a stronger positive association with bacterial vaginosis whereas MP2 was positively associated with trichomoniasis. MP1, in contrast to MP2 and other common BV-associated organisms, was not significantly excluded in pregnancy. In a cohort of 52 pregnant women, MP1 was both present and transcriptionally active in 75.4\u200a% of vaginal samples. Conversely, MP2 was largely absent in the pregnant cohort. This study provides insight into the evolutionary history, genomic potential and predicted functional role of two clinically relevant vaginal microbial taxa.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.000526","type":"journal-article","created":{"date-parts":[[2021,12,13]],"date-time":"2021-12-13T11:16:16Z","timestamp":1639394176000},"source":"Crossref","is-referenced-by-count":20,"title":["Unique roles of vaginal Megasphaera phylotypes in reproductive health"],"prefix":"10.1099","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6919-2632","authenticated-orcid":false,"given":"Abigail L.","family":"Glascock","sequence":"first","affiliation":[{"name":"Life Sciences, Virginia Commonwealth University, Richmond, VA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole R.","family":"Jimenez","sequence":"additional","affiliation":[{"name":"Center for Microbiome Engineering and Data Analysis, Virginia Commonwealth University, Richmond, VA, USA"},{"name":"Department of Microbiology & Immunology, Virginia Commonwealth University, Richmond, VA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sam","family":"Boundy","sequence":"additional","affiliation":[{"name":"Department of Microbiology & Immunology, Virginia Commonwealth University, Richmond, VA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8978-8495","authenticated-orcid":false,"given":"Vishal N.","family":"Koparde","sequence":"additional","affiliation":[{"name":"Life Sciences, Virginia Commonwealth University, Richmond, VA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0423-8422","authenticated-orcid":false,"given":"J. Paul","family":"Brooks","sequence":"additional","affiliation":[{"name":"Department of Supply Chain Management and Analytics, Virginia Commonwealth University, Richmond, VA, USA"},{"name":"Center for Microbiome Engineering and Data Analysis, Virginia Commonwealth University, Richmond, VA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Edwards","sequence":"additional","affiliation":[{"name":"Department of Statistical Sciences and Operations Research, Virginia Commonwealth University, Richmond, VA, USA"},{"name":"Center for Microbiome Engineering and Data Analysis, Virginia Commonwealth University, Richmond, VA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerome F.","family":"Strauss III","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, VA, USA"},{"name":"Center for Microbiome Engineering and Data Analysis, Virginia Commonwealth University, Richmond, VA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1052-1988","authenticated-orcid":false,"given":"Kimberly K.","family":"Jefferson","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, VA, USA"},{"name":"Center for Microbiome Engineering and Data Analysis, Virginia Commonwealth University, Richmond, VA, USA"},{"name":"Department of Microbiology & Immunology, Virginia Commonwealth University, Richmond, VA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4327-0193","authenticated-orcid":false,"given":"Myrna G.","family":"Serrano","sequence":"additional","affiliation":[{"name":"Center for Microbiome Engineering and Data Analysis, Virginia Commonwealth University, Richmond, VA, USA"},{"name":"Department of Microbiology & Immunology, Virginia Commonwealth University, Richmond, VA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4621-3987","authenticated-orcid":false,"given":"Gregory A.","family":"Buck","sequence":"additional","affiliation":[{"name":"Department of Microbiology & Immunology, Virginia Commonwealth University, Richmond, VA, USA"},{"name":"Department of Computer Science, Virginia Commonwealth University, Richmond, VA, USA"},{"name":"Center for Microbiome Engineering and Data Analysis, Virginia Commonwealth University, Richmond, VA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer M.","family":"Fettweis","sequence":"additional","affiliation":[{"name":"Department of Microbiology & Immunology, Virginia Commonwealth University, Richmond, VA, USA"},{"name":"Department of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, VA, USA"},{"name":"Center for Microbiome Engineering and Data Analysis, Virginia Commonwealth University, Richmond, VA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1002611107"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3016.2001.00007.x"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0030633"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1002478"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1002601107"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1002\/bdrc.21113"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2017.04.001"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0000000000000972"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1001251"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/20.Supplement_2.S276"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2013.05.006"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1111\/ppe.12106"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2020.10.019"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02127-07"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02347-13"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00410-17"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01272-07"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit100"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(18)30058-6"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2018-053949"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-016-0161-6"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0036298"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2015.00566"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-019-0450-2"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0166794"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2019.00201"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2014.11.043"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.201100359"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02272-07"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1186\/2049-2618-2-18"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2010.03.001"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt703"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0060843"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.11.2478"},{"key":"R36"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9525(00)02024-2"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01228-07"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm160"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01688-14"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh340"},{"key":"R43","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp033"},{"key":"R44","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.000347"},{"key":"R45","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt1226"},{"key":"R46","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/27.23.4636"},{"key":"R47","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.12.2607"},{"key":"R48","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.5.955"},{"key":"R49","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq461"},{"key":"R50","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp985"},{"key":"R51","doi-asserted-by":"crossref","DOI":"10.1186\/1471-2105-4-41","article-title":"The COG database: an updated version includes eukaryotes","volume":"4","author":"Tatusov","year":"2003","journal-title":"BMC Bioinformatics"},{"key":"R52","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.1.281"},{"key":"R53","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.200790212"},{"key":"R54","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku1220"},{"key":"R55","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq321"},{"key":"R56","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu033"},{"key":"R57","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a026334"},{"key":"R58","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-7-439"},{"key":"R59","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-13-S8-S17"},{"key":"R60","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn879"},{"key":"R61","doi-asserted-by":"publisher","DOI":"10.1016\/S1071-5754(97)90044-9"},{"key":"R62","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2014.08.014"},{"key":"R63","doi-asserted-by":"publisher","DOI":"10.1504\/IJCBDD.2017.085409"},{"key":"R64","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp698"},{"key":"R65","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1007\/978-1-4939-7514-3_16","article-title":"Primer Extension, Capture, and On-Bead cDNA Ligation: An Efficient RNAseq Library Prep Method for Determining Reverse Transcription Termination Sites","volume":"1712","author":"Ordoukhanian","year":"2018","journal-title":"Methods Mol Biol Clifton NJ"},{"key":"R66","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1002358"},{"key":"R67","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1004557"},{"key":"R68","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.081034-0"},{"key":"R69","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-52-3-983"},{"key":"R70","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-21-2-187"},{"key":"R71","doi-asserted-by":"publisher","DOI":"10.4056\/sigs.4077819"},{"key":"R72","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.02378-0"},{"key":"R73","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0079353"},{"key":"R74","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01439-13"},{"key":"R75","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012411"},{"key":"R76","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2575"},{"key":"R77","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2018.10.049"},{"key":"R78","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.059774-0"},{"key":"R79","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00204-15"},{"key":"R80","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2011.00117"},{"key":"R81","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evx192"},{"key":"R82","doi-asserted-by":"publisher","DOI":"10.1007\/s11693-013-9127-1"},{"key":"R83","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.004702"},{"key":"R84","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2011.03.016"},{"key":"R85","first-page":"201","article-title":"Can known risk factors explain racial differences in the occurrence of bacterial vaginosis?","volume":"95","author":"Ness","year":"2003","journal-title":"J Natl Med Assoc"},{"key":"R86","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0181135"},{"key":"R87","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-55929-7"},{"key":"R88","doi-asserted-by":"publisher","DOI":"10.1038\/srep08988"},{"key":"R89","doi-asserted-by":"publisher","DOI":"10.1186\/2049-2618-2-4"},{"key":"R90","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0036466"},{"key":"R91","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0098514"},{"key":"R92","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-019-0465-8"},{"key":"R93","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03104-15"},{"key":"R94","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(92)91368-K"},{"key":"R95","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2017.02.020"},{"key":"R96","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12804"},{"key":"R97","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiw140"},{"key":"R98","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpobgyn.2018.03.006"},{"key":"R99","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2018.12.005"},{"key":"R100","doi-asserted-by":"publisher","DOI":"10.1038\/s41372-019-0343-8"},{"key":"R101","first-page":"641","article-title":"Integrative HMP (iHMP) Research Network Consortium","volume":"569","year":"2019","journal-title":"The Integrative Human Microbiome Project Nature"}],"container-title":["Microbial Genomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/mgen\/10.1099\/mgen.0.000526?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T15:28:22Z","timestamp":1702567702000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/mgen\/10.1099\/mgen.0.000526"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,24]]},"references-count":101,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2021,12,24]]}},"URL":"https:\/\/doi.org\/10.1099\/mgen.0.000526","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.08.18.246819","asserted-by":"object"}]},"ISSN":["2057-5858"],"issn-type":[{"value":"2057-5858","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,24]]}}}