{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:42:21Z","timestamp":1791852141645,"version":"4.3.4"},"reference-count":69,"publisher":"Microbiology Society","issue":"3","license":[{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000071","name":"National Institute of Child Health and Human Development","doi-asserted-by":"crossref","award":["F30HD094435"],"award-info":[{"award-number":["F30HD094435"]}],"id":[{"id":"10.13039\/100000071","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000912","name":"March of Dimes Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000912","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Center for Women\u2019s Infectious Disease Research"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,3,1]]},"abstract":"\n The vaginal microbiome plays an important role in human health and species of vaginal bacteria have been associated with reproductive disease. Strain-level variation is also thought to be important, but the diversity, structure and evolutionary history of vaginal strains is not as well characterized. We developed and validated an approach to measure strain variation from metagenomic data based on SNPs within the core genomes for six species of vaginal bacteria:\n \n \n Gardnerella vaginalis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n ,\n \n \n Lactobacillus crispatus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n ,\n \n \n Lactobacillus iners<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n ,\n \n \n Lactobacillus jensenii<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n ,\n \n \n Lactobacillus gasseri<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n and\n \n \n Atopobium vaginae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n . Despite inhabiting the same environment, strain diversity and structure varies across species. All species except\n \n \n L. iners<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n are characterized by multiple distinct groups of strains. Even so, strain diversity is lower in the\n \n \n Lactobacillus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n species, consistent with a more recent colonization of the human vaginal microbiome. Both strain diversity and the frequency of multi-strain samples is related to species-level diversity of the microbiome in which they occur, suggesting similar ecological factors influencing diversity within the vaginal niche. We conclude that the structure of strain-level variation provides both the motivation and means of testing whether strain-level differences contribute to the function and health consequences of the vaginal microbiome.\n <\/jats:p>","DOI":"10.1099\/mgen.0.000543","type":"journal-article","created":{"date-parts":[[2021,3,3]],"date-time":"2021-03-03T09:37:08Z","timestamp":1614764228000},"source":"Crossref","is-referenced-by-count":19,"title":["The structure and diversity of strain-level variation in vaginal bacteria"],"prefix":"10.1099","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5136-9735","authenticated-orcid":false,"given":"Brett A.","family":"Tortelli","sequence":"first","affiliation":[{"name":"Center for Women\u2019s Infectious Disease Research, Washington University School of Medicine, St. Louis, MO, USA"},{"name":"Department of Genetics, Washington University School of Medicine, St. Louis, MO, 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