{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:31:00Z","timestamp":1788049860710,"version":"build-2784847793"},"reference-count":34,"publisher":"Microbiology Society","issue":"6","license":[{"start":{"date-parts":[[2021,3,24]],"date-time":"2021-03-24T00:00:00Z","timestamp":1616544000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Medical Research Council","award":["MR\/L01632X\/1"],"award-info":[{"award-number":["MR\/L01632X\/1"]}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["100\/974\/C\/13\/Z"],"award-info":[{"award-number":["100\/974\/C\/13\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"crossref","award":["BB\/J004529\/1"],"award-info":[{"award-number":["BB\/J004529\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100010016","name":"Nottingham Trent University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100010016","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100013842","name":"Imperial Health Charity","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100013842","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,3,24]]},"abstract":"\n As part of the ongoing studies with clinically relevant\n \n \n Klebsiella<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n spp., we characterized the genomes of three clinical GES-5-positive ST138 strains originally identified as\n Klebsiella oxytoca. bla<\/jats:italic>\n OXY<\/jats:sub>\n gene, average nucleotide identity and phylogenetic analyses showed the strains to be\n \n \n Klebsiella michiganensis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n . Affiliation of the strains to ST138 led us to demonstrate that the current multi-locus sequence typing scheme for\n \n \n K. oxytoca<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n can be used to distinguish members of this genetically diverse complex of bacteria. The strains encoded the kleboxymycin biosynthetic gene cluster (BGC), previously only found in\n \n \n K. oxytoca<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n strains and one strain of\n \n \n Klebsiella grimontii<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n . The finding of this BGC, associated with antibiotic-associated haemorrhagic colitis, in\n \n \n K. michiganensis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n led us to carry out a wide-ranging study to determine the prevalence of this BGC in\n \n \n Klebsiella<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n spp. Of 7170 publicly available\n \n \n Klebsiella<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n genome sequences screened, 88 encoded the kleboxymycin BGC. All BGC-positive strains belonged to the\n \n \n K. oxytoca<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n complex, with strains of four (\n \n \n K. oxytoca<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n ,\n \n \n K. pasteurii<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n ,\n \n \n K. grimontii<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n ,\n \n \n K. michiganensis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n ) of the six species of complex found to encode the complete BGC. In addition to being found in\n \n \n K. grimontii<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n strains isolated from preterm infants, the BGC was found in\n \n \n K. oxytoca<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n and\n \n \n K. michiganensis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n metagenome-assembled genomes recovered from neonates. Detection of the kleboxymycin BGC across the\n \n \n K. oxytoca<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n complex may be of clinical relevance and this cluster should be included in databases characterizing virulence factors, in addition to those characterizing BGCs.\n <\/jats:p>","DOI":"10.1099\/mgen.0.000592","type":"journal-article","created":{"date-parts":[[2021,6,18]],"date-time":"2021-06-18T10:16:37Z","timestamp":1624011397000},"source":"Crossref","is-referenced-by-count":21,"title":["Improved molecular characterization of the Klebsiella oxytoca complex reveals the prevalence of the kleboxymycin biosynthetic gene cluster"],"prefix":"10.1099","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7279-2236","authenticated-orcid":false,"given":"Preetha","family":"Shibu","sequence":"first","affiliation":[{"name":"Present address: Berkshire and Surrey Pathology Services, Frimley Health NHS Trust, Wexham Park Hospital, Slough, UK"},{"name":"Life Sciences, 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