{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:36:55Z","timestamp":1790696215684,"version":"4.1.0"},"reference-count":77,"publisher":"Microbiology Society","issue":"8","license":[{"start":{"date-parts":[[2021,8,31]],"date-time":"2021-08-31T00:00:00Z","timestamp":1630368000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,8,31]]},"abstract":"Shigellosis in men who have sex with men (MSM) is caused by multidrug resistant Shigellae, exhibiting resistance to antimicrobials including azithromycin, ciprofloxacin and more recently the third-generation cephalosporins. We sequenced four bla<\/jats:italic>\n CTX-M-27<\/jats:sub>-positive MSM \n \n Shigella<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> isolates (2018\u201320) using Oxford Nanopore Technologies; three \n \n S. sonnei<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> (identified as two MSM clade 2, one MSM clade 5) and one \n \n S. flexneri<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> 3a, to explore AMR context. All \n \n S. sonnei<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> isolates harboured Tn7\/Int2 chromosomal integrons, whereas \n \n S. flexneri<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> 3a contained the \n \n Shigella<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> Resistance Locus. All strains harboured IncFII pKSR100-like plasmids (67-83kbp); where present bla<\/jats:italic>\n CTX-M-27<\/jats:sub> was located on these plasmids flanked by IS26<\/jats:italic> and IS903B<\/jats:italic>, however bla<\/jats:italic>\n CTX-M-27<\/jats:sub> was lost in \n \n S. flexneri<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> 3a during storage between Illumina and Nanopore sequencing. IncFII AMR regions were mosaic and likely reorganised by IS26<\/jats:italic>; three of the four plasmids contained azithromycin-resistance genes erm(B<\/jats:italic>) and mph(A<\/jats:italic>) and one harboured the pKSR100 integron. Additionally, all \n \n S. sonnei<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> isolates possessed a large IncB\/O\/K\/Z plasmid, two of which carried aph(3\u2019)-Ib\/aph(6)-Id\/sul2<\/jats:italic> and tet(A<\/jats:italic>). Monitoring the transmission of mobile genetic elements with co-located AMR determinants is necessary to inform empirical treatment guidance and clinical management of MSM-associated shigellosis.<\/jats:p>","DOI":"10.1099\/mgen.0.000644","type":"journal-article","created":{"date-parts":[[2021,8,24]],"date-time":"2021-08-24T13:16:20Z","timestamp":1629810980000},"source":"Crossref","is-referenced-by-count":12,"title":["Acquisition and loss of CTX-M plasmids in Shigella species associated with MSM transmission in the UK"],"prefix":"10.1099","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5265-4629","authenticated-orcid":false,"given":"Rebecca K.","family":"Locke","sequence":"first","affiliation":[{"name":"University of Bath, Claverton Down Campus, Bath, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9436-067X","authenticated-orcid":false,"given":"David R.","family":"Greig","sequence":"additional","affiliation":[{"name":"Division of Infection and Immunity, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, EH25 9RG, UK"},{"name":"Gastrointestinal Reference Services, Public Health England, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Jenkins","sequence":"additional","affiliation":[{"name":"Gastrointestinal Reference Services, Public Health England, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tim J.","family":"Dallman","sequence":"additional","affiliation":[{"name":"Division of Infection and Immunity, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, EH25 9RG, UK"},{"name":"Gastrointestinal Reference Services, Public Health England, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0835-7971","authenticated-orcid":false,"given":"Lauren A.","family":"Cowley","sequence":"additional","affiliation":[{"name":"University of Bath, Claverton Down Campus, Bath, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2015.12.021"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/159.6.1126"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.3201\/eid2302.161203"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00032-07"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki954"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.3201\/eid1611.090934"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)60844-2"},{"key":"R8","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-018-25764-3","article-title":"Genomic epidemiology of Shigella in the United Kingdom shows transmission of pathogen sublineages and determinants of antimicrobial resistance","volume":"8","author":"Baker","year":"2018","journal-title":"Scientific Reports"},{"key":"R9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2807\/1560-7917.ES2015.20.15.21097","article-title":"Intensified shigellosis epidemic associated with sexual transmission in men who have sex with men - Shigella fexneri and s. 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