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However, surveillance of non-cholera \n \n Vibrio<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> is limited both in public health and in food. Using a population- and market share-weighted study design, 211 prawn samples were collected and cultured for \n \n Vibrio<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> spp. Contamination was detected in 46\u200a% of samples, and multiple diverse \n \n Vibrio<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> isolates were obtained from 34\u200a% of positive samples. Whole genome sequencing (WGS) and phylogenetic analysis illustrated a comprehensive view of \n \n Vibrio<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> species diversity in prawns available at retail, with no known pathogenicity markers identified in \n \n Vibrio parahaemolyticus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and \n \n V. cholerae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. Antimicrobial resistance genes were found in 77\u200a% of isolates, and 12\u200a% carried genes conferring resistance to three or more drug classes. Resistance genes were found predominantly in \n \n V. parahaemolyticus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, though multiple resistance genes were also identified in \n \n V. cholerae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and \n \n V. vulnificus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. This study highlights the large diversity in \n \n Vibrio<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> derived from prawns at retail, even within a single sample. Although there was little evidence in this study that prawns are a major source of vibriosis in the UK, surveillance of non-cholera \n \n Vibrio<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> is very limited. This study illustrates the value of expanding WGS surveillance efforts of non-cholera Vibrios in the food chain to identify critical control points for food safety through the production system and to determine the full extent of the public health impact.<\/jats:p>","DOI":"10.1099\/mgen.0.000647","type":"journal-article","created":{"date-parts":[[2021,9,29]],"date-time":"2021-09-29T13:29:14Z","timestamp":1632922154000},"source":"Crossref","is-referenced-by-count":17,"title":["Whole genome sequencing reveals great diversity of Vibrio spp in prawns at retail"],"prefix":"10.1099","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7396-9969","authenticated-orcid":false,"given":"Nicol","family":"Janecko","sequence":"first","affiliation":[{"name":"Quadram Institute Bioscience, Norwich Research Park, Norwich NR4 7UQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel J.","family":"Bloomfield","sequence":"additional","affiliation":[{"name":"Quadram Institute Bioscience, Norwich Research Park, Norwich NR4 7UQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rapha\u00eblle","family":"Palau","sequence":"additional","affiliation":[{"name":"Quadram Institute Bioscience, Norwich Research Park, Norwich NR4 7UQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alison E.","family":"Mather","sequence":"additional","affiliation":[{"name":"Quadram Institute Bioscience, Norwich Research Park, Norwich NR4 7UQ, UK"},{"name":"Faculty of Medicine and Health Sciences, University of East Anglia, Norwich NR4 7TJ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-018-0005-8"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2020.107244"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1001923"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.002786"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X.JFP-17-366"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1016\/j.cll.2015.02.007"},{"key":"R7","first-page":"691","volume-title":"Vibrio: Introduction, including Vibrio parahaemolyticus, Vibrio vulnificus, and other Vibrio species. 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