{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:44:45Z","timestamp":1792003485774,"version":"4.4.0"},"reference-count":70,"publisher":"Microbiology Society","issue":"12","license":[{"start":{"date-parts":[[2021,12,24]],"date-time":"2021-12-24T00:00:00Z","timestamp":1640304000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001349","name":"National Medical Research Council","doi-asserted-by":"crossref","award":["NMRC\/CIRG\/1467\/2017"],"award-info":[{"award-number":["NMRC\/CIRG\/1467\/2017"]}],"id":[{"id":"10.13039\/501100001349","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001349","name":"National Medical Research Council","doi-asserted-by":"crossref","award":["CIRG19NOV-0024"],"award-info":[{"award-number":["CIRG19NOV-0024"]}],"id":[{"id":"10.13039\/501100001349","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100007652","name":"Singapore Millennium Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100007652","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100005606","name":"Genome Institute of Singapore","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005606","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,12,24]]},"abstract":"<jats:p>\n                    Group B\n                    <jats:italic>\n                      <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5605\" xlink:type=\"simple\">Streptococcus<\/jats:ext-link>\n                      <\/jats:named-content>\n                    <\/jats:italic>\n                    (GBS;\n                    <jats:italic>\n                      <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5609\" xlink:type=\"simple\">Streptococcus agalactiae<\/jats:ext-link>\n                      <\/jats:named-content>\n                    <\/jats:italic>\n                    ) is the most common cause of neonatal meningitis and a rising cause of sepsis in adults. Recently, it has also been shown to cause foodborne disease. As with many other bacteria, the polysaccharide capsule of GBS is antigenic, enabling its use for strain serotyping. Recent advances in DNA sequencing have made sequence-based typing attractive (as has been implemented for several other bacteria, including\n                    <jats:italic>\n                      <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3093\" xlink:type=\"simple\">Escherichia coli<\/jats:ext-link>\n                      <\/jats:named-content>\n                    <\/jats:italic>\n                    ,\n                    <jats:italic>\n                      <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.10789\" xlink:type=\"simple\">Klebsiella pneumoniae<\/jats:ext-link>\n                      <\/jats:named-content>\n                    <\/jats:italic>\n                    species complex,\n                    <jats:italic>\n                      <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5606\" xlink:type=\"simple\">Streptococcus pyogenes<\/jats:ext-link>\n                      <\/jats:named-content>\n                    <\/jats:italic>\n                    , and others). For GBS, existing WGS-based serotyping systems do not provide complete coverage of all known GBS serotypes (specifically including subtypes of serotype III), and none are simultaneously compatible with the two most common data types, raw short reads and assembled sequences. Here, we create a serotyping database (GBS-SBG, GBS Serotyping by Genome Sequencing), with associated scripts and running instructions, that can be used to call all currently described GBS serotypes, including subtypes of serotype III, using both direct short-read- and assembly-based typing. We achieved higher concordance using GBS-SBG on a previously reported data set of 790 strains. We further validated GBS-SBG on a new set of 572 strains, achieving 99.8% concordance with PCR-based molecular serotyping using either short-read- or assembly-based typing. The GBS-SBG package is publicly available and will hopefully accelerate and simplify serotyping by sequencing for GBS.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.000688","type":"journal-article","created":{"date-parts":[[2021,12,13]],"date-time":"2021-12-13T09:15:59Z","timestamp":1639386959000},"source":"Crossref","is-referenced-by-count":26,"title":["GBS-SBG - GBS Serotyping by Genome Sequencing"],"prefix":"10.1099","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0195-1097","authenticated-orcid":false,"given":"Suma","family":"Tiruvayipati","sequence":"first","affiliation":[{"name":"Infectious Diseases Translational Research Programme, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4609-0841","authenticated-orcid":false,"given":"Wen Ying","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Tan Tock Seng Hospital, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0975-2244","authenticated-orcid":false,"given":"Timothy M. 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