{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T01:10:46Z","timestamp":1791853846377,"version":"4.3.4"},"reference-count":47,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003107","name":"Bundesministerium f\u00fcr Gesundheit","doi-asserted-by":"crossref","award":["ZMVI1-2518FSB709"],"award-info":[{"award-number":["ZMVI1-2518FSB709"]}],"id":[{"id":"10.13039\/501100003107","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,11,1]]},"abstract":"\n Non-typhoidal\n \n \n Salmonella enterica<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n is an important gastrointestinal pathogen causing a considerable burden of disease. Resistance to third generation cephalosporins poses a serious threat for treatment of severe infections. In this study occurrence, phylogenetic relationship, and mechanisms of third generation cephalosporin resistance were investigated for clinical non-typhoidal\n \n \n S. enterica<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n isolates in Germany. From 2017 to 2019, we detected 168 unique clinical\n \n \n S. enterica<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n isolates with phenotypic resistance to third generation cephalosporins in a nation-wide surveillance. Compared to previous years, we observed a significant (\n P<\/jats:italic>\n =0.0002) and consistent increase in resistant isolates from 0.41\u200a% in 2005 to 1.71\u200a% in 2019. In total, 34 different serovars were identified, most often\n S<\/jats:italic>\n . Infantis (\n n<\/jats:italic>\n =41; 24.4 %),\n S<\/jats:italic>\n . Typhimurium (\n n<\/jats:italic>\n =27; 16.1 %),\n S<\/jats:italic>\n . Kentucky (\n n<\/jats:italic>\n =21; 12.5 %), and\n S<\/jats:italic>\n . Derby (\n n<\/jats:italic>\n =17; 10.1 %). Whole genome analyses revealed extended-spectrum \u03b2-lactamase (ESBL) genes as main cause for third generation cephalosporin resistance, and most prevalent were\n bla<\/jats:italic>\n CTX-M-1<\/jats:sub>\n (\n n<\/jats:italic>\n =55),\n bla<\/jats:italic>\n CTX-M-14<\/jats:sub>\n (\n n<\/jats:italic>\n =25), and\n bla<\/jats:italic>\n CTX-M-65<\/jats:sub>\n (\n n<\/jats:italic>\n =23). There was no strict correlation between serovar, phylogenetic lineage, and ESBL type but some serovar\/ESBL gene combinations were detected frequently, such as\n bla<\/jats:italic>\n CTX-M-1<\/jats:sub>\n and\n bla<\/jats:italic>\n CTX-M-65<\/jats:sub>\n in\n S<\/jats:italic>\n . Infantis or\n bla<\/jats:italic>\n CTX-M-14b<\/jats:sub>\n in\n S<\/jats:italic>\n . Kentucky. The ESBL genes were mainly located on plasmids, including IncI, IncA\/C variants, emerging pESI variants, and a novel\n bla<\/jats:italic>\n CTX-M-1<\/jats:sub>\n harbouring plasmid. We conclude that third generation cephalosporin resistance is on the rise among clinical\n \n \n S. enterica<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n isolates in Germany, and occurrence in various\n \n \n S. enterica<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n serovars is most probably due to multiple acquisition events of plasmids.\n <\/jats:p>","DOI":"10.1099\/mgen.0.000698","type":"journal-article","created":{"date-parts":[[2021,10,25]],"date-time":"2021-10-25T06:17:28Z","timestamp":1635142648000},"source":"Crossref","is-referenced-by-count":24,"title":["Third generation cephalosporin resistance in clinical non-typhoidal Salmonella enterica in Germany and emergence of bla\n CTX-M-harbouring pESI plasmids"],"prefix":"10.1099","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4807-3244","authenticated-orcid":false,"given":"Michael","family":"Pietsch","sequence":"first","affiliation":[{"name":"Unit for Enteropathogenic Bacteria and 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