{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T02:31:01Z","timestamp":1792722661453,"version":"4.5.0"},"reference-count":72,"publisher":"Microbiology Society","issue":"12","license":[{"start":{"date-parts":[[2021,12,24]],"date-time":"2021-12-24T00:00:00Z","timestamp":1640304000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,12,24]]},"abstract":"<jats:p>Uropathogenic <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3093\" xlink:type=\"simple\">Escherichia coli<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> (UPEC) UTI89 is a well-characterized strain, which has mainly been used to study UPEC virulence during urinary tract infection (UTI). However, little is known on UTI89 key fitness-factors during growth in lab media and during UTI. Here, we used a transposon-insertion-sequencing approach (TraDIS) to reveal the UTI89 essential-genes for <jats:italic>in vitro<\/jats:italic> growth and fitness-gene-sets for growth in Luria broth (LB) and EZ-MOPS medium without glucose, as well as for human bacteriuria and mouse cystitis. A total of 293 essential genes for growth were identified and the set of fitness-genes was shown to differ depending on the growth media. A modified, previously validated UTI murine model, with administration of glucose prior to infection was applied. Selected fitness-genes for growth in urine and mouse-bladder colonization were validated using deletion-mutants. Novel fitness-genes, such as <jats:italic>tusA, corA<\/jats:italic> and <jats:italic>rfaG;<\/jats:italic> involved in sulphur-acquisition, magnesium-uptake, and LPS-biosynthesis, were proved to be important during UTI. Moreover, <jats:italic>rfaG<\/jats:italic> was confirmed as relevant in both niches, and therefore it may represent a target for novel UTI-treatment\/prevention strategies.<\/jats:p>","DOI":"10.1099\/mgen.0.000719","type":"journal-article","created":{"date-parts":[[2021,12,20]],"date-time":"2021-12-20T15:15:49Z","timestamp":1640013349000},"source":"Crossref","is-referenced-by-count":21,"title":["Genome-wide analysis of fitness-factors in uropathogenic Escherichia coli during growth in laboratory media and during urinary tract infections"],"prefix":"10.1099","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5459-8325","authenticated-orcid":false,"given":"Vanesa","family":"Garc\u00eda","sequence":"first","affiliation":[{"name":"Laboratorio de Referencia de Escherichia coli (LREC), Departamento de Microbiolox\u00eda e Parasitolox\u00eda, Facultade de Veterinaria, Universidade de Santiago de Compostela (USC), Lugo, Spain"},{"name":"Department of Veterinary and Animal 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