{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T14:12:51Z","timestamp":1790863971223,"version":"4.1.0"},"reference-count":114,"publisher":"Microbiology Society","issue":"4","license":[{"start":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T00:00:00Z","timestamp":1650931200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000288","name":"Royal Society","doi-asserted-by":"crossref","award":["RSWF\\R1\\180013"],"award-info":[{"award-number":["RSWF\\R1\\180013"]}],"id":[{"id":"10.13039\/501100000288","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100014013","name":"UK Research and Innovation","doi-asserted-by":"crossref","award":["85336"],"award-info":[{"award-number":["85336"]}],"id":[{"id":"10.13039\/100014013","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"crossref","award":["EP\/V043811\/1"],"award-info":[{"award-number":["EP\/V043811\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Institute for Heatlh Research","award":["NIHR2000907"],"award-info":[{"award-number":["NIHR2000907"]}]},{"DOI":"10.13039\/501100000288","name":"Royal Society","doi-asserted-by":"crossref","award":["CHG\\R1\\170036"],"award-info":[{"award-number":["CHG\\R1\\170036"]}],"id":[{"id":"10.13039\/501100000288","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["217303\/Z\/19\/Z"],"award-info":[{"award-number":["217303\/Z\/19\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100014013","name":"UK Research and Innovation","doi-asserted-by":"crossref","award":["20197"],"award-info":[{"award-number":["20197"]}],"id":[{"id":"10.13039\/100014013","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"crossref","award":["BB\/T001240\/1"],"award-info":[{"award-number":["BB\/T001240\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["R01-AI116811"],"award-info":[{"award-number":["R01-AI116811"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["101285"],"award-info":[{"award-number":["101285"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"crossref","award":["BB\/V011278\/1"],"award-info":[{"award-number":["BB\/V011278\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["R21AI138074"],"award-info":[{"award-number":["R21AI138074"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,4,26]]},"abstract":"\n \n \n Wolbachia<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n is a genus of obligate bacterial endosymbionts that infect a diverse range of arthropod species as well as filarial nematodes, with its single described species,\n \n \n Wolbachia pipientis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n , divided into several \u2018supergroups\u2019 based on multilocus sequence typing.\n \n \n Wolbachia<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n strains in mosquitoes have been shown to inhibit the transmission of human pathogens, including\n Plasmodium<\/jats:italic>\n malaria parasites and arboviruses. Despite their large host range,\n \n \n Wolbachia<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n strains within the major malaria vectors of the\n Anopheles gambiae<\/jats:italic>\n and\n Anopheles funestus<\/jats:italic>\n complexes appear at low density, established solely on PCR-based methods. Questions have been raised as to whether this represents a true endosymbiotic relationship. However, recent definitive evidence for two distinct, high-density strains of supergroup B\n \n \n Wolbachia<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n within\n Anopheles demeilloni<\/jats:italic>\n and\n Anopheles moucheti<\/jats:italic>\n has opened exciting possibilities to explore naturally occurring\n \n \n Wolbachia<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n endosymbionts in\n Anopheles<\/jats:italic>\n for biocontrol strategies to block\n Plasmodium<\/jats:italic>\n transmission. Here, we utilize genomic analyses to demonstrate that both\n \n \n Wolbachia<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n strains have retained all key metabolic and transport pathways despite their smaller genome size, with this reduction potentially attributable to degenerated prophage regions. Even with this reduction, we confirmed the presence of cytoplasmic incompatibility (CI) factor genes within both strains, with\n w<\/jats:italic>\n AnD maintaining intact copies of these genes while the\n cifB<\/jats:italic>\n gene was interrupted in\n w<\/jats:italic>\n AnM, so functional analysis is required to determine whether\n w<\/jats:italic>\n AnM can induce CI. Additionally, phylogenetic analysis indicates that these\n \n \n Wolbachia<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n strains may have been introduced into these two\n Anopheles<\/jats:italic>\n species via horizontal transmission events, rather than by ancestral acquisition and subsequent loss events in the\n Anopheles gambiae<\/jats:italic>\n species complex. These are the first\n \n \n Wolbachia<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n genomes, to our knowledge, that enable us to study the relationship between natural strain\n Plasmodium<\/jats:italic>\n malaria parasites and their anopheline hosts.\n <\/jats:p>","DOI":"10.1099\/mgen.0.000805","type":"journal-article","created":{"date-parts":[[2022,4,21]],"date-time":"2022-04-21T11:17:26Z","timestamp":1650539846000},"source":"Crossref","is-referenced-by-count":11,"title":["Wolbachia endosymbionts in two Anopheles species indicates independent acquisitions and lack of prophage elements"],"prefix":"10.1099","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1583-9541","authenticated-orcid":false,"given":"Shannon","family":"Quek","sequence":"first","affiliation":[{"name":"Department of Tropical Disease Biology, Centre for Neglected Tropical Diseases, Liverpool School of Tropical Medicine, Liverpool, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4495-2615","authenticated-orcid":false,"given":"Louise","family":"Cerdeira","sequence":"additional","affiliation":[{"name":"Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0298-2808","authenticated-orcid":false,"given":"Claire L.","family":"Jeffries","sequence":"additional","affiliation":[{"name":"Department of Disease Control, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0735-2741","authenticated-orcid":false,"given":"Sean","family":"Tomlinson","sequence":"additional","affiliation":[{"name":"Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3545-012X","authenticated-orcid":false,"given":"Thomas","family":"Walker","sequence":"additional","affiliation":[{"name":"Department of Disease Control, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7567-7185","authenticated-orcid":false,"given":"Grant L.","family":"Hughes","sequence":"additional","affiliation":[{"name":"Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, UK"},{"name":"Department of Tropical Disease Biology, Centre for Neglected Tropical Diseases, Liverpool School of Tropical Medicine, Liverpool, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4413-3756","authenticated-orcid":false,"given":"Eva","family":"Heinz","sequence":"additional","affiliation":[{"name":"Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, UK"},{"name":"Department of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.6084\/m9.figshare.19576432"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1969"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2015.0249"},{"key":"R4","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1002\/ece3.2742","article-title":"Incidence of Wolbachia in aquatic insects","volume":"7","author":"Sazama","year":"2017","journal-title":"Ecol Evol"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.000724"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2021.03.006"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-007-0055-8"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00731-06"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0911476107"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1409284111"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1038\/nature21391"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2017.7"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msh211"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evy012"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msaa209"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2018.12.004"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evy159"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ento.42.1.587"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genet.41.110306.130354"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1126\/science.1165326"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1038\/nature10355"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1038\/nature10356"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0471-x"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1800650115"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1008221"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2968"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2013.11.004"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.3390\/v7112903"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2030243"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.12688\/gatesopenres.13122.1"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002043"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1126\/science.1236192"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00366"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-7-336"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762011000900026"},{"key":"R36","article-title":"Is Anopheles gambiae a natural host of Wolbachia?","volume":"10","author":"Chrostek","year":"2019","journal-title":"mMBio"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2021.03.056"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4985"},{"key":"R39","doi-asserted-by":"crossref","first-page":"12566","DOI":"10.1073\/pnas.1716181114","article-title":"Effect of naturally occurring Wolbachia in Anopheles gambiae s.l. mosquitoes from Mali on Plasmodium falciparum malaria transmission","volume":"114","author":"Gomes","year":"2017","journal-title":"Proc Natl Acad Sci USA"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-018-2559-z"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.1111\/eva.12804"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-020-04277-x"},{"key":"R43","doi-asserted-by":"publisher","DOI":"10.12688\/wellcomeopenres.14765.2"},{"key":"R44","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2010-11-4-r41"},{"key":"R45","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1005944"},{"key":"R46","doi-asserted-by":"publisher","DOI":"10.1101\/gr.2289704"},{"key":"R47","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbs012"},{"key":"R48","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-19016-0"},{"key":"R49","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv351"},{"key":"R50","article-title":"Wgsim for simulating sequence reads from a reference genome","author":"Li","year":"2011"},{"key":"R51","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp352"},{"key":"R52","article-title":"Snippy: fast bacterial variant calling from NGS reads","author":"Seeman","year":"2015"},{"key":"R53","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku1196"},{"key":"R54","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msu300"},{"key":"R55","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-015-0721-2"},{"key":"R56","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst010"},{"key":"R57","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0163962"},{"key":"R58","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4285"},{"key":"R59","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btx364"},{"key":"R60","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw313"},{"key":"R61","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw569"},{"key":"R62","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky995"},{"key":"R63","article-title":"Howard Hughes Medical Institute. HMMER: biosequence analysis using profile hidden Markov models","year":"2013"},{"key":"R64","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"R65","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr039"},{"key":"R66","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1093\/bib\/bbz104","article-title":"Pathway Tools version 23.0 update: software for pathway\/genome informatics and systems biology","volume":"22","author":"Karp","year":"2021","journal-title":"Brief Bioinform"},{"key":"R67","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz862"},{"key":"R68","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbx085"},{"key":"R69","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msx148"},{"key":"R70","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm321"},{"key":"R71","volume-title":"ggplot2: Elegant Graphics for Data Analysis (https:\/\/ggplot2.tidyverse.org)","author":"Wickham","year":"2016"},{"key":"R72","doi-asserted-by":"publisher","DOI":"10.1080\/10635150701472164"},{"key":"R73","doi-asserted-by":"publisher","DOI":"10.1093\/sysbio\/syq010"},{"key":"R74","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1111\/2041-210X.12628","article-title":"Ggtree: an R package for visualization and annotation of phylogenetic trees with their covariates and other associated data","volume":"8","author":"Yu","year":"2017","journal-title":"Methods Ecol Evol"},{"key":"R75","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-646-7_10"},{"key":"R76","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw387"},{"key":"R77","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr703"},{"key":"R78","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2011-12-3-r30"},{"key":"R79","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evab111"},{"key":"R80","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-42454-w"},{"key":"R81","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.7.2699"},{"key":"R82","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1995.0117"},{"key":"R83","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.027581-0"},{"key":"R84","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00377-08"},{"key":"R85","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2013.12.019"},{"key":"R86","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0204736"},{"key":"R87","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2009.12.011"},{"key":"R88","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msl171"},{"key":"R89","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms13155"},{"key":"R90","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1003381"},{"key":"R91","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0250051"},{"key":"R92","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.02237"},{"key":"R93","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003224"},{"key":"R94","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evz025"},{"key":"R95","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msn133"},{"key":"R96","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.595629"},{"key":"R97","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0030121"},{"key":"R98","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000487"},{"key":"R99","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/iyaa007"},{"key":"R100","doi-asserted-by":"publisher","DOI":"10.1111\/evo.13745"},{"key":"R101","doi-asserted-by":"publisher","DOI":"10.1038\/36017"},{"key":"R102","doi-asserted-by":"publisher","DOI":"10.1038\/sj.hdy.6800177"},{"key":"R103","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.61989"},{"key":"R104","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.116.196238"},{"key":"R105","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.1999.tb04529.x"},{"key":"R106","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.1994.tb02192.x"},{"key":"R107","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1996.0016"},{"key":"R108","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0004425"},{"key":"R109","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.2008.00550.x"},{"key":"R110","doi-asserted-by":"publisher","DOI":"10.1038\/sj.hdy.6800739"},{"key":"R111","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003607"},{"key":"R112","doi-asserted-by":"publisher","DOI":"10.1111\/evo.13506"},{"key":"R113","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006815"},{"key":"R114","doi-asserted-by":"publisher","DOI":"10.1126\/science.1209609"}],"container-title":["Microbial Genomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/mgen\/10.1099\/mgen.0.000805?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T15:25:43Z","timestamp":1702567543000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/mgen\/10.1099\/mgen.0.000805"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,26]]},"references-count":114,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,4,26]]}},"URL":"https:\/\/doi.org\/10.1099\/mgen.0.000805","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.11.15.468614","asserted-by":"object"}]},"ISSN":["2057-5858"],"issn-type":[{"value":"2057-5858","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,26]]}}}