{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:43:28Z","timestamp":1791852208385,"version":"4.3.4"},"reference-count":39,"publisher":"Microbiology Society","issue":"5","license":[{"start":{"date-parts":[[2022,5,27]],"date-time":"2022-05-27T00:00:00Z","timestamp":1653609600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["20fk0108132j0001"],"award-info":[{"award-number":["20fk0108132j0001"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["21fk0108604j0001"],"award-info":[{"award-number":["21fk0108604j0001"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["JP21K16947"],"award-info":[{"award-number":["JP21K16947"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["JP18K07112"],"award-info":[{"award-number":["JP18K07112"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,5,27]]},"abstract":"<jats:p>\n                  <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.10789\" xlink:type=\"simple\">Klebsiella pneumoniae<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> (Kp) has emerged as a global life-threatening pathogen owing to its multidrug resistance and hypervirulence phenotype. Several fatal outbreaks of carbapenem-resistant hypervirulent Kp have been reported recently. Hypermucoviscosity (HMV) is a phenotype commonly associated with hypervirulence of Kp, which is usually regulated by <jats:italic>rmpA<\/jats:italic> or <jats:italic>rmpA2<\/jats:italic> (regulators of the mucoid phenotype). Here, we found that temperature was important in the HMV phenotype of Kp, and the impact of temperature on HMV was not uniform among strains. We investigated the HMV phenotype at 37\u2009\u00b0C and room temperature (20\u201325\u2009\u00b0C) in 170 clinically isolated hypermucoviscous Kp strains in Japan and analysed the association between the HMV phenotype, virulence genes and antimicrobial resistance (AMR) genes. String length distribution at different temperatures was correlated with the genomic population of Kp. The strains carrying <jats:italic>rmpA<\/jats:italic>\/<jats:italic>rmpA2<\/jats:italic> frequently showed the HMV phenotype at 37\u2009\u00b0C, while the strains negative for these genes tended to show the HMV phenotype at room temperature. Hypervirulent Kp clusters carrying <jats:italic>rmpA<\/jats:italic>\/<jats:italic>rmpA2<\/jats:italic> without extended-spectrum beta-lactamases (ESBL)\/carbapenemases produced higher string lengths at 37\u2009\u00b0C than at room temperature, and were mostly isolated from the respiratory tract. Other HMV strains showed distinct characteristics of not carrying <jats:italic>rmpA<\/jats:italic>\/<jats:italic>rmpA2<\/jats:italic> but were positive for ESBL\/carbapenemases, with a higher string length at room temperature than at 37\u2009\u00b0C, and were frequently isolated from bloodstream infections. In total, 21 (13.5\u200a%) HMV isolates carried ESBL and carbapenemases, among which five isolates were carbapenem-resistant hypervirulent Kp with a pLVPK-like plasmid (an epidemic virulence plasmid) and a pKPI-6-like plasmid (an epidemic <jats:italic>bla<\/jats:italic>\n                  <jats:sub>IMP-6<\/jats:sub>-bearing plasmid in Japan), suggesting the convergence of worldwide hypervirulence and epidemic AMR in Japan.<\/jats:p>","DOI":"10.1099\/mgen.0.000827","type":"journal-article","created":{"date-parts":[[2022,5,27]],"date-time":"2022-05-27T17:16:20Z","timestamp":1653671780000},"source":"Crossref","is-referenced-by-count":21,"title":["Genomic epidemiology and temperature dependency of hypermucoviscous Klebsiella pneumoniae in Japan"],"prefix":"10.1099","volume":"8","author":[{"given":"Mi Nguyen-Tra","family":"Le","sequence":"first","affiliation":[{"name":"Department of Bacteriology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, 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Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masato","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koji","family":"Yahara","sequence":"additional","affiliation":[{"name":"Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsuneko","family":"Terachi","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Osaka Police Hospital, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kana","family":"Sawa","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Osaka Police Hospital, Osaka, 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