{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T01:36:22Z","timestamp":1772156182814,"version":"3.50.1"},"reference-count":35,"publisher":"Microbiology Society","issue":"7","license":[{"start":{"date-parts":[[2022,7,29]],"date-time":"2022-07-29T00:00:00Z","timestamp":1659052800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,7,29]]},"abstract":"\n \n \n Listeria monocytogenes<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n (\n Lm<\/jats:italic>\n ) is a bacterial pathogen that causes listeriosis in immunocompromised individuals, particularly pregnant women. Several virulence factors support the intracellular lifecycle of\n Lm<\/jats:italic>\n and facilitate cell-to-cell spread, allowing it to occupy multiple niches within the host and cross-protective barriers, including the placenta. One family of virulence factors, internalins, contributes to\n Lm<\/jats:italic>\n pathogenicity by inducing specific uptake and conferring tissue tropism. Over 25 internalins have been identified thus far, but only a few have been extensively studied. Internalins contain leucine-rich repeat (LRR) domains that enable protein-protein interactions, allowing\n Lm<\/jats:italic>\n to bind host proteins. Notably, other\n \n \n Listeria<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n species express internalins but cannot colonize human hosts, prompting questions regarding the evolution of internalins within the genus\n \n \n Listeria<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n . Internalin P (InlP) promotes placental colonization through interaction with the host protein afadin. Although prior studies of InlP have begun to elucidate its role in\n Lm<\/jats:italic>\n pathogenesis, there remains a lack of information regarding homologs in other\n \n \n Listeria<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n species. Here, we have used a computational evolutionary approach to identify InlP homologs in additional\n \n \n Listeria<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n species. We found that\n \n \n Listeria ivanovii londoniensis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n (\n Liv<\/jats:italic>\n ) and\n \n \n Listeria seeligeri<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n (\n Ls<\/jats:italic>\n ) encode InlP homologs. We also found InlP-like homologs in\n \n \n Listeria innocua<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n and the recently identified species\n \n \n Listeria costaricensis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n . All newly identified homologs lack the full-length LRR6 and LRR7 domains found in\n Lm<\/jats:italic>\n \u2019s InlP. These findings are informative regarding the evolution of one key\n Lm<\/jats:italic>\n virulence factor, InlP, and serve as a springboard for future evolutionary studies of\n Lm<\/jats:italic>\n pathogenesis as well as mechanistic studies of\n \n \n Listeria<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n internalins.\n <\/jats:p>","DOI":"10.1099\/mgen.0.000828","type":"journal-article","created":{"date-parts":[[2022,7,29]],"date-time":"2022-07-29T10:16:57Z","timestamp":1659089817000},"source":"Crossref","is-referenced-by-count":1,"title":["Novel internalin P homologs in Listeria"],"prefix":"10.1099","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2408-6502","authenticated-orcid":false,"given":"Kayla N.","family":"Conner","sequence":"first","affiliation":[{"name":"Institute for Quantitative Health Science and Engineering, Michigan State University, East Lansing, MI, USA"},{"name":"Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0532-3338","authenticated-orcid":false,"given":"Joseph T.","family":"Burke","sequence":"additional","affiliation":[{"name":"Department of Pathobiology and Diagnostic Investigation, Michigan State University, East Lansing, MI, USA"},{"name":"Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, USA"},{"name":"Genomics and Molecular Genetics Undergraduate Program, College of Natural Science, Michigan State University, East Lansing, MI, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7443-925X","authenticated-orcid":false,"given":"Janani","family":"Ravi","sequence":"additional","affiliation":[{"name":"Department of Pathobiology and Diagnostic Investigation, Michigan State University, East Lansing, MI, USA"},{"name":"Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, USA"},{"name":"Department of Biomedical Informatics, Center for Health Artificial Intelligence, University of Colorado Anschutz Medical Campus, Aurora, CO, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7686-9819","authenticated-orcid":false,"given":"Jonathan W.","family":"Hardy","sequence":"additional","affiliation":[{"name":"Institute for Quantitative Health Science and Engineering, Michigan State University, East Lansing, MI, USA"},{"name":"Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)60074-4"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00801-17"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00397-18"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-016-7552-2"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.3.584-640.2001"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens9070528"},{"key":"R7","article-title":"Listeria (Listeriosis)"},{"key":"R8","article-title":"Listeria During 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