{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T11:40:41Z","timestamp":1788262841263,"version":"build-2803163510"},"reference-count":104,"publisher":"Microbiology Society","issue":"8","license":[{"start":{"date-parts":[[2022,8,23]],"date-time":"2022-08-23T00:00:00Z","timestamp":1661212800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,8,23]]},"abstract":"<jats:p>\n                    Insertion sequences (ISs) and other transposable elements are associated with the mobilization of antibiotic resistance determinants and the modulation of pathogenic characteristics. In this work, we aimed to investigate the association between ISs and antibiotic resistance genes, and their role in the dissemination and modification of the antibiotic-resistant phenotype. To that end, we leveraged fully resolved\n                    <jats:italic>\n                      <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5535\" xlink:type=\"simple\">Enterococcus faecium<\/jats:ext-link>\n                      <\/jats:named-content>\n                    <\/jats:italic>\n                    and\n                    <jats:italic>\n                      <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5526\" xlink:type=\"simple\">Enterococcus faecalis<\/jats:ext-link>\n                      <\/jats:named-content>\n                    <\/jats:italic>\n                    genomes of isolates collected over 5\u2009days from an inpatient with prolonged bacteraemia. Isolates from both species harboured similar IS family content but showed significant species-dependent differences in copy number and arrangements of ISs throughout their replicons. Here, we describe two inter-specific IS-mediated recombination events and IS-mediated excision events in plasmids of\n                    <jats:italic>\n                      <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5535\" xlink:type=\"simple\">E. faecium<\/jats:ext-link>\n                      <\/jats:named-content>\n                    <\/jats:italic>\n                    isolates. We also characterize a novel arrangement of the ISs in a Tn1546-like transposon in\n                    <jats:italic>\n                      <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5526\" xlink:type=\"simple\">E. faecalis<\/jats:ext-link>\n                      <\/jats:named-content>\n                    <\/jats:italic>\n                    isolates likely implicated in a vancomycin genotype\u2013phenotype discrepancy. Furthermore, an extended analysis revealed a novel association between daptomycin resistance mutations in\n                    <jats:italic>liaSR<\/jats:italic>\n                    genes and a putative composite transposon in\n                    <jats:italic>\n                      <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5535\" xlink:type=\"simple\">E. faecium<\/jats:ext-link>\n                      <\/jats:named-content>\n                    <\/jats:italic>\n                    , offering a new paradigm for the study of daptomycin resistance and novel insights into its dissemination. In conclusion, our study highlights the role ISs and other transposable elements play in the rapid adaptation and response to clinically relevant stresses such as aggressive antibiotic treatment in enterococci.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.000855","type":"journal-article","created":{"date-parts":[[2022,8,3]],"date-time":"2022-08-03T11:17:26Z","timestamp":1659525446000},"source":"Crossref","is-referenced-by-count":13,"title":["Insertion sequences and other mobile elements associated with antibiotic resistance genes in Enterococcus isolates from an inpatient with prolonged bacteraemia"],"prefix":"10.1099","volume":"8","author":[{"given":"Zulema","family":"Udaondo","sequence":"first","affiliation":[{"name":"Department of Biomedical Informatics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaleb Z.","family":"Abram","sequence":"additional","affiliation":[{"name":"Department of Biomedical Informatics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atul","family":"Kothari","sequence":"additional","affiliation":[{"name":"Arkansas Dept of Health, Healthcare Associated Infections and Outbreak Response Sections, Little Rock, AR 72205, USA"},{"name":"Department of Biomedical Informatics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2681-3950","authenticated-orcid":false,"given":"Se-Ran","family":"Jun","sequence":"additional","affiliation":[{"name":"Department of Biomedical Informatics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2017.06.001"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0107-z"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00464-19"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.GPP3-0053-2018"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0147478"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00211-16"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-62371-7"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000048"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1186\/s13073-021-00868-0"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(21)00236-6"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.04.027"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-21749-5"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000488"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00728"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.03284-19"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000277"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkw312"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00182"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14515"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-12825-y"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6976.12067"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00088-17"},{"key":"R23","article-title":"Mechanisms of DNA Transposition. 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