{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T04:32:25Z","timestamp":1779251545326,"version":"3.51.4"},"reference-count":94,"publisher":"Microbiology Society","issue":"2","license":[{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010418","name":"Defence Science and Technology Laboratory","doi-asserted-by":"crossref","award":["DSTLX-1000123071"],"award-info":[{"award-number":["DSTLX-1000123071"]}],"id":[{"id":"10.13039\/100010418","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,2,1]]},"abstract":"<jats:p>\n                  <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.2391\" xlink:type=\"simple\">Coxiella burnetii<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> is an intracellular pathogen responsible for causing Q fever in humans, a disease with varied presentations ranging from a mild flu-like sickness to a debilitating illness that can result in endocarditis. The intracellular lifestyle of <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.2391\" xlink:type=\"simple\">C. burnetii<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> is unique, residing in an acidic phagolysosome-like compartment within host cells. An understanding of the core molecular biology of <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.2391\" xlink:type=\"simple\">C. burnetii<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> will greatly increase our understanding of <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.2391\" xlink:type=\"simple\">C. burnetii<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> growth, survival and pathogenesis. We used transposon-directed insertion site sequencing (TraDIS) to reveal <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.2391\" xlink:type=\"simple\">C. burnetii<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> Nine Mile Phase II genes fundamental for growth and <jats:italic>in vitro<\/jats:italic> survival. Screening a transposon library containing &gt;10\u200a000 unique transposon mutants revealed 512 predicted essential genes. Essential routes of synthesis were identified for the mevalonate pathway, as well as peptidoglycan and biotin synthesis. Some essential genes identified (e.g. predicted type IV secretion system effector genes) are typically considered to be associated with <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.2391\" xlink:type=\"simple\">C. burnetii<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> virulence, a caveat concerning the axenic media used in the study. Investigation into the conservation of the essential genes identified revealed that 78\u200a% are conserved across all <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.2391\" xlink:type=\"simple\">C. burnetii<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> strains sequenced to date, which probably play critical functions. This is the first report of a whole genome transposon screen in <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.2391\" xlink:type=\"simple\">C. burnetii<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> that has been undertaken for the identification of essential genes.<\/jats:p>","DOI":"10.1099\/mgen.0.000944","type":"journal-article","created":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T16:17:10Z","timestamp":1675268230000},"source":"Crossref","is-referenced-by-count":7,"title":["Identification of essential genes in Coxiella burnetii"],"prefix":"10.1099","volume":"9","author":[{"given":"Georgie","family":"Metters","sequence":"first","affiliation":[{"name":"Defence Science and Technology Laboratories, CBR Division, Porton Down, Salisbury SP4 0JQ, 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