{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T03:22:19Z","timestamp":1784085739257,"version":"3.55.0"},"reference-count":61,"publisher":"Microbiology Society","issue":"5","license":[{"start":{"date-parts":[[2023,5,12]],"date-time":"2023-05-12T00:00:00Z","timestamp":1683849600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,5,12]]},"abstract":"<jats:p>Increased colonization by antimicrobial-resistant organisms is closely associated with international travel. This study investigated the diversity of mobile genetic elements involved with antimicrobial resistance (AMR) gene carriage in extended-spectrum beta-lactamase (ESBL)-producing <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3093\" xlink:type=\"simple\">Escherichia coli<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> that colonized travellers to Laos. Long-read sequencing was used to reconstruct complete plasmid sequences from 48 isolates obtained from the daily stool samples of 23 travellers over a 3\u2009week period. This method revealed a collection of 105 distinct plasmids, 38.1\u200a% (<jats:italic>n<\/jats:italic>=40) of which carried AMR genes. The plasmids in this population were diverse, mostly unreported and included 38 replicon types, with F-type plasmids (<jats:italic>n<\/jats:italic>=23) the most prevalent amongst those carrying AMR genes. Fine-scale analysis of all plasmids identified numerous AMR gene contexts and emphasized the importance of IS elements, specifically members of the IS<jats:italic>6\/<\/jats:italic>IS<jats:italic>26<\/jats:italic> family, in the evolution of complex multidrug resistance regions. We found a concerning convergence of ESBL and colistin resistance determinants, with three plasmids from two different F-type lineages carrying <jats:italic>bla<\/jats:italic>\n                  <jats:sub>CTX-M<\/jats:sub> and <jats:italic>mcr<\/jats:italic> genes. The extensive diversity seen here highlights the worrying probability that stable new vehicles for AMR will evolve in <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3093\" xlink:type=\"simple\">E. coli<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> populations that can disseminate internationally through travel networks.<\/jats:p>","DOI":"10.1099\/mgen.0.001000","type":"journal-article","created":{"date-parts":[[2023,5,12]],"date-time":"2023-05-12T15:18:31Z","timestamp":1683904711000},"source":"Crossref","is-referenced-by-count":16,"title":["The highly diverse plasmid population found in Escherichia coli colonizing travellers to Laos and its role in antimicrobial resistance gene carriage"],"prefix":"10.1099","volume":"9","author":[{"given":"Ann E.","family":"Snaith","sequence":"first","affiliation":[{"name":"Institute of Microbiology and Infection, College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven J.","family":"Dunn","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Infection, College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Moran","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Infection, College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul N.","family":"Newton","sequence":"additional","affiliation":[{"name":"Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK"},{"name":"Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, UK"},{"name":"Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Microbiology Laboratory, Mahosot Hospital, Rue Mahosot, Vientiane, Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A. B.","family":"Dance","sequence":"additional","affiliation":[{"name":"Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK"},{"name":"Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, UK"},{"name":"Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Microbiology Laboratory, Mahosot Hospital, Rue Mahosot, Vientiane, Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viengmon","family":"Davong","sequence":"additional","affiliation":[{"name":"Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Microbiology Laboratory, Mahosot Hospital, Rue Mahosot, Vientiane, Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esther","family":"Kuenzli","sequence":"additional","affiliation":[{"name":"University of Basel, Basel, Switzerland"},{"name":"Department of Medicine, Swiss Tropical and Public Health Institute of Basel, Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anu","family":"Kantele","sequence":"additional","affiliation":[{"name":"Human Microbiome Research Program, Faculty of Medicine, Haartmaninkatu 4, 00014 University of Helsinki, Helsinki, Finland"},{"name":"Multidisciplinary Center of Excellence in Antimicrobial Resistance Research, University of Helsinki, Helsinki, Finland"},{"name":"Meilahti Infectious Diseases and Vaccine Research Center, MeVac, Biomedicum 1, Haartmaninkatu 8, Helsinki University Hospital and University of Helsinki, Helsinki 00290, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jukka","family":"Corander","sequence":"additional","affiliation":[{"name":"Department of Mathematics and Statistics, Helsinki Institute of Information Technology, University of Helsinki, Helsinki, Finland"},{"name":"Department of Biostatistics, Faculty of Medicine, University of Oslo, Oslo, Norway"},{"name":"Parasites and Microbes Programme, Wellcome Sanger Institute, Wellcome Genome Campus, Cambridge, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3099-630X","authenticated-orcid":false,"given":"Alan","family":"McNally","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Infection, College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1086\/499406"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmaid.2019.101547"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02408-18"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.3390\/tropicalmed6010011"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1093\/jtm\/taw101"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(16)30319-X"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(20)30224-X"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2012.01.005"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.4103\/0974-777X.68531"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02162-15"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1322678111"},{"key":"R12","article-title":"WHO Model List of Essential Medicines","year":"2017"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1177\/2049936115621709"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2019.1735-1746"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-15539-7"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.78.082907.145923"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S199844"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008331"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S48820"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00088-17"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkx488"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1128\/msystems.00831-21"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-021-00879-y"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2020.0579"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-9877-7_20"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkaa345"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1002\/cpbi.102"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty149"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1005595"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv383"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0072-8"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkx327"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.12688\/wellcomeopenres.14826.1"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq347"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj014"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msu300"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-020-02090-4"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btx433"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2016.02.002"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2017.03.007"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1235"},{"key":"R43","doi-asserted-by":"publisher","DOI":"10.1186\/s13100-019-0182-3"},{"key":"R44","doi-asserted-by":"publisher","DOI":"10.1038\/s41396-021-00926-w"},{"key":"R45","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-138-7-1345"},{"key":"R46","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1009919"},{"key":"R47","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2016.11.001"},{"key":"R48","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.21.7032-7068.2004"},{"key":"R49","doi-asserted-by":"publisher","DOI":"10.1016\/bs.ampbs.2017.02.001"},{"key":"R50","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-019-0623-2"},{"key":"R51","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph16010022"},{"key":"R52","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics9110746"},{"key":"R53","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2015.09.001"},{"key":"R54","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0178623"},{"key":"R55","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fny100"},{"key":"R56","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01867.x"},{"key":"R57","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01801-14"},{"key":"R58","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkh251"},{"key":"R59","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00866-10"},{"key":"R60","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01407-08"},{"key":"R61","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00038-16"}],"container-title":["Microbial Genomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/mgen\/10.1099\/mgen.0.001000?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,12]],"date-time":"2023-05-12T15:18:34Z","timestamp":1683904714000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/mgen\/10.1099\/mgen.0.001000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,12]]},"references-count":61,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,5,12]]}},"URL":"https:\/\/doi.org\/10.1099\/mgen.0.001000","relation":{},"ISSN":["2057-5858"],"issn-type":[{"value":"2057-5858","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,12]]}}}