{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:48:44Z","timestamp":1787010524370,"version":"build-2736575974"},"reference-count":42,"publisher":"Microbiology Society","issue":"4","license":[{"start":{"date-parts":[[2023,4,28]],"date-time":"2023-04-28T00:00:00Z","timestamp":1682640000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100018710","name":"National Center of Competence in Research AntiResist","doi-asserted-by":"crossref","award":["180541"],"award-info":[{"award-number":["180541"]}],"id":[{"id":"10.13039\/100018710","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Ministry of Agriculture and Rural Development","award":["16-18-0002"],"award-info":[{"award-number":["16-18-0002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,4,28]]},"abstract":"\n Brucellosis is a worldwide zoonosis with important public health, animal health and economic implications.\n \n \n Brucella melitensis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n , commonly associated with small ruminants, is an emerging bovine pathogen in dairy farms. We analysed all\n \n \n B. melitensis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n outbreaks affecting dairy farms in Israel since 2006, combining traditional and genomic epidemiology to explore the public health implications of this One Health challenge. Whole-genome sequencing was applied to bovine and related human\n \n \n B. melitensis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n isolates from dairy farm outbreaks. cgMLST-based and SNP-based typing was integrated with epidemiological and investigation data. A secondary analysis combining the bovine-human isolates with endemic human isolates from southern Israel was performed. A total of 92 isolates from dairy cows and related human cases originating from 18 epidemiological clusters were analysed. Most genomic and epi-clusters were congruent, but sequencing showed relatedness between apparently unrelated farm outbreaks. Nine secondary human infections were also genomically confirmed. The bovine-human cohort appeared intermixed with 126 endemic human isolates in southern Israel. We show a persistent and widespread circulation of\n \n \n B. melitensis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n in dairy farms in Israel with secondary occupational human infection. The genomic epidemiology also uncovered cryptic connections between outbreaks. A regional connection between bovine and endemic human brucellosis cases points to a common reservoir, most probably local small ruminant herds. Control of humans and bovine brucellosis is inseparable. Epidemiological and microbiological surveillance and implementation of control measures across the entire range of farm animals is needed to mitigate this public health challenge.\n <\/jats:p>","DOI":"10.1099\/mgen.0.001014","type":"journal-article","created":{"date-parts":[[2023,4,28]],"date-time":"2023-04-28T10:17:17Z","timestamp":1682677037000},"source":"Crossref","is-referenced-by-count":13,"title":["Public and animal health risks associated with spillover of Brucella melitensis into dairy farms"],"prefix":"10.1099","volume":"9","author":[{"given":"Svetlana","family":"Bardenstein","sequence":"first","affiliation":[{"name":"Kimron Veterinary Institute, Ministry of Agriculture and Rural Development, Beit Dagan, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8315-1204","authenticated-orcid":false,"given":"Daniel","family":"Grupel","sequence":"additional","affiliation":[{"name":"Department of Health Policy and Management, School of Public Health, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel"},{"name":"Internal Medicine B, Soroka University Medical Center, Beer Sheva, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shlomo E.","family":"Blum","sequence":"additional","affiliation":[{"name":"Kimron Veterinary Institute, Ministry of Agriculture and Rural Development, Beit Dagan, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yair","family":"Motro","sequence":"additional","affiliation":[{"name":"Department of Health Policy and Management, School of Public Health, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob","family":"Moran-Gilad","sequence":"additional","affiliation":[{"name":"Department of Health Policy and Management, School of Public Health, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(06)70382-6"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra050570"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-020-02283-w"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2014.11.014"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.12691\/ajeid-5-2-2"},{"key":"R6","volume-title":"Zoonoses and Communicable Diseases Common to Man and Animals","author":"Acha pedro","year":"2001"},{"key":"R7","article-title":"Brucellosis Outbreak Traced to Commercially Sold Camel Milk through Whole-Genome Sequencing, Israel- Volume 27, Number 6\u2014June 2021 - Emerging Infectious Diseases journal - CDC. Emerg Infect Dis [Internet] 27(6):1728\u201331","author":"Bardenstein","year":"2021"},{"key":"R8","article-title":"Ministry of Agriculture and Rural Development","year":"2019"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.4172\/2161-0711.1000575"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci5010028"},{"key":"R11","article-title":"Brucella melitensis infection discovered in cattle for first time, goats also infected | American Veterinary Medical Association"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-022-03155-1"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00517-18"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2021.635547"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0175425"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-26906-3"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10020238"},{"key":"R18","article-title":"Regulations for sampling cattle in dairy farms for brucellosis","year":"2018"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0112963"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty266"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.12688\/wellcomeopenres.14826.1"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-11-595"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000166"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-11-119"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1101\/gr.232397.117"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0229863"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10030619"},{"key":"R30","article-title":"GitHub - NCBI\/datasets: NCBI Datasets is an experimental resource for finding and building datasets"},{"key":"R31","article-title":"GitHub - tseemann\/snippy: Rapid haploid variant calling and core genome alignment"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku1196"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msaa131"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4285"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty633"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1002\/imt2.56"},{"key":"R37","first-page":"160","volume-title":"ggplot2: Elegant Graphics for Data Analysis (2nd ed.)","author":"Villanueva","year":"2019"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-020-02090-4"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.20506\/rst.32.1.2197"},{"key":"R41","first-page":"359","article-title":"Recent trends in human brucellosis in Israel","volume":"13","author":"Anis","year":"2011","journal-title":"Isr Med Assoc J"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21207749"}],"container-title":["Microbial Genomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/mgen\/10.1099\/mgen.0.001014?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,28]],"date-time":"2023-04-28T10:17:23Z","timestamp":1682677043000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/mgen\/10.1099\/mgen.0.001014"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,28]]},"references-count":42,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2023,4,28]]}},"URL":"https:\/\/doi.org\/10.1099\/mgen.0.001014","relation":{},"ISSN":["2057-5858"],"issn-type":[{"value":"2057-5858","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,28]]}}}