{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T22:08:21Z","timestamp":1766182101157,"version":"3.40.5"},"reference-count":47,"publisher":"Microbiology Society","issue":"5","license":[{"start":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T00:00:00Z","timestamp":1684886400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,5,24]]},"abstract":"<jats:p>Antibiotic resistance of <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.10886\" xlink:type=\"simple\">Mycobacterium tuberculosis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> (Mtb) is a major public health concern worldwide. Therefore, it is of great significance to characterize the mutational pathways by which susceptible Mtb evolves into drug resistance. In this study, we used laboratory evolution to explore the mutational pathways of aminoglycoside resistance. The level of resistance in amikacin inducing Mtb was also associated with changes in susceptibility to other anti-tuberculosis drugs such as isoniazid, levofloxacin and capreomycin. Whole-genome sequencing (WGS) revealed that the induced resistant Mtb strains had accumulated diverse mutations. We found that <jats:italic>rrs<\/jats:italic> A1401G was the predominant mutation in aminoglycoside-resistant clinical Mtb isolates from Guangdong. In addition, this study provided global insight into the characteristics of the transcriptome in four representative induced strains and revealed that <jats:italic>rrs<\/jats:italic> mutated and unmutated aminoglycoside-resistant Mtb strains have different transcriptional profiles. WGS analysis and transcriptional profiling of Mtb strains during evolution revealed that Mtb strains harbouring <jats:italic>rrs<\/jats:italic> A1401G have an evolutionary advantage over other drug-resistant strains under the pressure of aminoglycosides because of their ultra-high resistance level and low physiological impact on the strain. The results of this study should advance our understanding of aminoglycoside resistance mechanisms.<\/jats:p>","DOI":"10.1099\/mgen.0.001022","type":"journal-article","created":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T16:17:00Z","timestamp":1684945020000},"source":"Crossref","is-referenced-by-count":4,"title":["Whole-genome sequencing and transcriptome-characterized in vitro evolution of aminoglycoside resistance in Mycobacterium tuberculosis"],"prefix":"10.1099","volume":"9","author":[{"given":"Wenjing","family":"Wei","sequence":"first","affiliation":[{"name":"Center for Tuberculosis Control of Guangdong Province, Key Laboratory of Translational Medicine of Guangdong, Guangzhou 510630, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3834-6368","authenticated-orcid":false,"given":"Yuchuan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Center for Tuberculosis Control of Guangdong Province, Key Laboratory of Translational Medicine of Guangdong, Guangzhou 510630, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenchen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center for Tuberculosis Control of Guangdong Province, Key Laboratory of Translational Medicine of Guangdong, Guangzhou 510630, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meiling","family":"Yu","sequence":"additional","affiliation":[{"name":"Center for Tuberculosis Control of Guangdong Province, Key Laboratory of Translational Medicine of Guangdong, Guangzhou 510630, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuhua","family":"Wu","sequence":"additional","affiliation":[{"name":"Center for Tuberculosis Control of Guangdong Province, Key Laboratory of Translational Medicine of Guangdong, Guangzhou 510630, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liuyue","family":"Xu","sequence":"additional","affiliation":[{"name":"Center for Tuberculosis Control of Guangdong Province, Key Laboratory of Translational Medicine of Guangdong, Guangzhou 510630, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kehao","family":"Peng","sequence":"additional","affiliation":[{"name":"Center for Tuberculosis Control of Guangdong Province, Key Laboratory of Translational Medicine of Guangdong, Guangzhou 510630, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhilong","family":"Wu","sequence":"additional","affiliation":[{"name":"Foshan Fourth People's Hospital, Foshan 528000, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanxia","family":"Li","sequence":"additional","affiliation":[{"name":"Foshan Fourth People's Hospital, Foshan 528000, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuezhi","family":"Wang","sequence":"additional","affiliation":[{"name":"Foshan Fourth People's Hospital, Foshan 528000, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"year":"2021","key":"R1","article-title":"GLOBAL TUBERCULOSIS REPORT"},{"key":"R2","first-page":"685","article-title":"Analysis of monitoring results of tuberculosis drug-resistance in Guangdong Province from 2016 to 2020","volume":"44","author":"Chen","year":"2022","journal-title":"Chinese J Antituberculosis"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)31316-7"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-021-05947-6"},{"year":"2016","key":"R5","article-title":"WHO Treatment Guidelines for Drug-Resistant Tuberculosis, 2016 Update. 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