{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T19:30:40Z","timestamp":1792092640121,"version":"4.4.0"},"reference-count":63,"publisher":"Microbiology Society","issue":"6","license":[{"start":{"date-parts":[[2023,6,9]],"date-time":"2023-06-09T00:00:00Z","timestamp":1686268800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"crossref","award":["FL150100038"],"award-info":[{"award-number":["FL150100038"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,6,9]]},"abstract":"<jats:p>\n                  <jats:italic>Dehalobacterium formicoaceticum<\/jats:italic> is recognized for its ability to anaerobically ferment dichloromethane (DCM), and a catabolic model has recently been proposed. <jats:italic>D. formicoaceticum<\/jats:italic> is currently the only axenic representative of its class, the <jats:italic>Dehalobacteriia<\/jats:italic>, according to the Genome Taxonomy Database. However, substantial additional diversity has been revealed in this lineage through culture-independent exploration of anoxic habitats. Here we performed a comparative analysis of 10 members of the <jats:italic>Dehalobacteriia,<\/jats:italic> representing three orders, and infer that anaerobic DCM degradation appears to be a recently acquired trait only present in some members of the order <jats:italic>Dehalobacteriales<\/jats:italic>. Inferred traits common to the class include the use of amino acids as carbon and energy sources for growth, energy generation via a remarkable range of putative electron-bifurcating protein complexes and the presence of S-layers. The ability of <jats:italic>D. formicoaceticum<\/jats:italic> to grow on serine without DCM was experimentally confirmed and a high abundance of the electron-bifurcating protein complexes and S-layer proteins was noted when this organism was grown on DCM. We suggest that members of the <jats:italic>Dehalobacteriia<\/jats:italic> are low-abundance fermentative scavengers in anoxic habitats.<\/jats:p>","DOI":"10.1099\/mgen.0.001039","type":"journal-article","created":{"date-parts":[[2023,6,9]],"date-time":"2023-06-09T12:17:05Z","timestamp":1686313025000},"source":"Crossref","is-referenced-by-count":8,"title":["A comparative genome analysis of the Bacillota (Firmicutes) class Dehalobacteriia"],"prefix":"10.1099","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0580-1600","authenticated-orcid":false,"given":"Young C.","family":"Song","sequence":"first","affiliation":[{"name":"The University of Queensland, School of Chemistry and Molecular Biosciences, Australian Centre for Ecogenomics, St Lucia, Queensland, 4072, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie I.","family":"Holland","sequence":"additional","affiliation":[{"name":"Present address: School of Engineering & Physical Sciences, Heriot-Watt University, Edinburgh, UK"},{"name":"School of Civil and Environmental Engineering, UNSW Sydney, Sydney, NSW, 2052, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Lee","sequence":"additional","affiliation":[{"name":"School of Civil and Environmental Engineering, UNSW Sydney, Sydney, NSW, 2052, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gao","family":"Chen","sequence":"additional","affiliation":[{"name":"Center for Environmental Biotechnology, Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank E.","family":"L\u00f6ffler","sequence":"additional","affiliation":[{"name":"Oak Ridge National Laboratory, Biosciences Division, Oak Ridge, Tennessee, USA"},{"name":"Department of Microbiology, Department of Bioengineering and Soil Science, University of Tennessee, Knoxville, Tennessee, USA"},{"name":"Center for Environmental Biotechnology, Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Manefield","sequence":"additional","affiliation":[{"name":"School of Civil and Environmental Engineering, UNSW Sydney, Sydney, NSW, 2052, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"Hugenholtz","sequence":"additional","affiliation":[{"name":"The University of Queensland, School of Chemistry and Molecular Biosciences, Australian Centre for Ecogenomics, St Lucia, Queensland, 4072, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2642-1319","authenticated-orcid":false,"given":"Ulrike","family":"Kappler","sequence":"additional","affiliation":[{"name":"The University of Queensland, School of Chemistry and Molecular Biosciences, St Lucia, Queensland, 4072, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1038"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0539-7"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.01.001"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001920"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.004332"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms15962"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-211-99323-1"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2011.02524.x"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1134\/S0026261710010091"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1007\/s002030050362"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1038\/s41396-020-00881-y"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.7775"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02768-18"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1111\/gcb.16068"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2009.153"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1290"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1219"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbz104"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-515-2"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2015.11.006"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku1221"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-020-01158-y"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-71038-2"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2018.09.364"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btab007"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4285"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.8b01583"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)67912-8"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1080\/10643389609388492"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2003.07.018"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2019.08.032"},{"key":"R34","article-title":"R Core Team. R Foundation for Statistical Computing","year":"2017"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1128\/JB.02158-12"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2008.01679.x"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2006.00029.x"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-52-5-1729"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1021\/es4020184"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00659"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-020-01679-y"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.01045-14"},{"key":"R43","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0098425"},{"key":"R44","doi-asserted-by":"publisher","DOI":"10.1038\/nature07336"},{"key":"R45","doi-asserted-by":"publisher","DOI":"10.1038\/nature05414"},{"key":"R46","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0504978102"},{"key":"R47","doi-asserted-by":"publisher","DOI":"10.1038\/4441022a"},{"key":"R48","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0069184"},{"key":"R49","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12168"},{"key":"R50","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2012.61"},{"key":"R51","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00038-17"},{"key":"R52","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2013.09.002"},{"key":"R53","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1716667115"},{"key":"R54","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1321542111"},{"key":"R55","doi-asserted-by":"publisher","DOI":"10.1021\/ja508647u"},{"key":"R56","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1912289117"},{"key":"R57","doi-asserted-by":"publisher","DOI":"10.1038\/s41396-019-0479-8"},{"key":"R58","doi-asserted-by":"publisher","DOI":"10.1007\/s00239-011-9447-2"},{"key":"R59","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01582-08"},{"key":"R60","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12405"},{"key":"R61","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.663468"},{"key":"R62","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3213"},{"key":"R63","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00054-10"}],"container-title":["Microbial Genomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/mgen\/10.1099\/mgen.0.001039?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,9]],"date-time":"2023-06-09T12:17:08Z","timestamp":1686313028000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/mgen\/10.1099\/mgen.0.001039"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,9]]},"references-count":63,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,6,9]]}},"URL":"https:\/\/doi.org\/10.1099\/mgen.0.001039","relation":{},"ISSN":["2057-5858"],"issn-type":[{"value":"2057-5858","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,9]]}}}