{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T16:53:08Z","timestamp":1783529588283,"version":"3.55.0"},"reference-count":65,"publisher":"Microbiology Society","issue":"7","license":[{"start":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T00:00:00Z","timestamp":1690156800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"crossref","award":["DE190100238"],"award-info":[{"award-number":["DE190100238"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,7,24]]},"abstract":"\n \n \n Chlamydia psittaci<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> is a globally distributed veterinary pathogen with zoonotic potential. Although \n \n C. psittaci<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> infections have been reported in various hosts, isolation and culture of \n \n Chlamydia<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> is challenging, hampering efforts to produce contemporary global \n \n C. psittaci<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> genomes. This is particularly evident in the lack of avian \n \n C. psittaci<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> genomes from Australia and New Zealand. In this study, we used culture-independent probe-based whole-genome sequencing to expand the global \n \n C. psittaci<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> genome catalogue. Here, we provide new \n \n C. psittaci<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> genomes from two pigeons, six psittacines, and novel hosts such as the Australian bustard (Ardeotis australis<\/jats:italic>) and sooty shearwater (Ardenna grisea<\/jats:italic>) from Australia and New Zealand. We also evaluated \n \n C. psittaci<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> genetic diversity using multilocus sequence typing (MLST) and major outer membrane protein (omp<\/jats:italic>A) genotyping on additional \n \n C. psittaci<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>-positive samples from various captive avian hosts and field isolates from Australasia. We showed that the first \n \n C. psittaci<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> genomes sequenced from New Zealand parrots and pigeons belong to the clonal sequence type (ST)24 and diverse \u2018pigeon-type\u2019 ST27 clade, respectively. Australian parrot-derived strains also clustered in the ST24 group, whereas the novel ST332 strain from the Australian bustard clustered in a genetically diverse clade of strains from a fulmar, parrot, and livestock. MLST and omp<\/jats:italic>A genotyping revealed ST24\/omp<\/jats:italic>A genotype A in wild and captive parrots and a sooty shearwater, whilst \u2018pigeon-types\u2019 (ST27\/35 and omp<\/jats:italic>A genotypes B\/E) were found in pigeons and other atypical hosts, such as captive parrots, a little blue penguin\/Koror\u0101 (Eudyptula minor<\/jats:italic>) and a zebra finch (Taeniopygia guttata castanotis<\/jats:italic>) from Australia and New Zealand. This study provides new insights into the global phylogenomic diversity of \n \n C. psittaci<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and further demonstrates the multi-host generalist capacity of this pathogen.<\/jats:p>","DOI":"10.1099\/mgen.0.001072","type":"journal-article","created":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T16:17:36Z","timestamp":1690215456000},"source":"Crossref","is-referenced-by-count":10,"title":["Whole-genome sequencing of Chlamydia psittaci from Australasian avian hosts: A genomics approach to a pathogen that still ruffles feathers"],"prefix":"10.1099","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3284-2168","authenticated-orcid":false,"given":"Vasilli","family":"Kasimov","sequence":"first","affiliation":[{"name":"University of the Sunshine Coast, Centre for Bioinnovation, Sippy Downs, Sunshine Coast, QLD 4557, 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