{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T05:13:00Z","timestamp":1785388380702,"version":"3.55.0"},"reference-count":61,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T00:00:00Z","timestamp":1696377600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"crossref","award":["BB\/T014644\/1"],"award-info":[{"award-number":["BB\/T014644\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,10,4]]},"abstract":"\n \n \n Staphylococcus capitis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n is a frequent cause of late-onset sepsis in neonates admitted to Neonatal Intensive Care Units (NICU). One clone of\n \n \n S. capitis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n , NRCS-A has been isolated from NICUs globally although the reasons for the global success of this clone are not well understood.\n <\/jats:p>\n \n We analysed a collection of\n \n \n S. capitis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n colonising babies admitted to two NICUs, one in the UK and one in Germany as well as corresponding pathological clinical isolates. Genome analysis identified a population structure of three groups; non-NRCS-A isolates, NRCS-A isolates, and a group of \u2018proto NRCS-A\u2019 \u2013 isolates closely related to NRCS-A but not associated with neonatal infection. All bloodstream isolates belonged to the NRCS-A group and were indistinguishable from strains carried on the skin or in the gut. NRCS-A isolates showed increased tolerance to chlorhexidine and antibiotics relative to the other\n \n \n S. capitis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n as well as enhanced ability to grow at higher pH values. Analysis of the pangenome of 138 isolates identified characteristic\n nsr<\/jats:italic>\n and\n tarJ<\/jats:italic>\n genes in both the NRCS-A and proto groups. A CRISPR-cas system was only seen in NRCS-A isolates which also showed enrichment of genes for metal acquisition and transport.\n <\/jats:p>\n \n We found evidence for transmission of\n \n \n S. capitis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n NRCS-A within NICU, with related isolates shared between babies and multiple acquisitions by some babies. Our data show NRCS-A strains commonly colonise uninfected babies in NICU representing a potential reservoir for potential infection. This work provides more evidence that adaptation to survive in the gut and on skin facilitates spread of NRCS-A, and that metal acquisition and tolerance may be important to the biology of NRCS-A. Understanding how NRCS-A survives in NICUs can help develop infection control procedures against this clone.\n <\/jats:p>","DOI":"10.1099\/mgen.0.001106","type":"journal-article","created":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T06:16:48Z","timestamp":1696400208000},"source":"Crossref","is-referenced-by-count":8,"title":["Characterisation of neonatal Staphylococcus capitis NRCS-A isolates compared with non NRCS-A Staphylococcus capitis from neonates and adults"],"prefix":"10.1099","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4045-4798","authenticated-orcid":false,"given":"Heather","family":"Felgate","sequence":"first","affiliation":[{"name":"Norwich Medical School, University of East Anglia (UEA), Norwich, UK"},{"name":"Quadram Institute Bioscience, Norwich Research Park, NR4 7UQ, Norwich, 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