{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T01:36:23Z","timestamp":1772156183968,"version":"3.50.1"},"reference-count":55,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T00:00:00Z","timestamp":1698624000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000272","name":"National Institute for Health and Care Research","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"crossref"}]},{"name":"NIHR Cambridge Biomedical Research Centre"},{"DOI":"10.13039\/501100000691","name":"Academy of Medical Sciences","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000691","id-type":"DOI","asserted-by":"crossref"}]},{"name":"NIHR Cambridge Biomedical Research Centre"},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["215515\/Z\/19\/Z"],"award-info":[{"award-number":["215515\/Z\/19\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,10,30]]},"abstract":"\n \n \n Escherichia coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n is a ubiquitous component of the human gut microbiome, but is also a common pathogen, causing around 40,\u200a000 bloodstream infections (BSI) in the United Kingdom (UK) annually. The number of\n \n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n BSI has increased over the last decade in the UK, and emerging antimicrobial resistance (AMR) profiles threaten treatment options. Here, we combined clinical, epidemiological, and whole genome sequencing data with high content imaging to characterise over 300\n \n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n isolates associated with BSI in a large teaching hospital in the East of England. Overall, only a limited number of sequence types (ST) were responsible for the majority of organisms causing invasive disease. The most abundant (20\u200a% of all isolates) was ST131, of which around 90\u200a% comprised the pandemic O25b:H4 group. ST131-O25b:H4 isolates were frequently multi-drug resistant (MDR), with a high prevalence of extended spectrum \u03b2-lactamases (ESBL) and fluoroquinolone resistance. There was no association between AMR phenotypes and the source of\n \n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n bacteraemia or whether the infection was healthcare-associated. Several clusters of ST131 were genetically similar, potentially suggesting a shared transmission network. However, there was no clear epidemiological associations between these cases, and they included organisms from both healthcare-associated and non-healthcare-associated origins. The majority of ST131 isolates exhibited strong binding with an anti-O25b antibody, raising the possibility of developing rapid diagnostics targeting this pathogen. In summary, our data suggest that a restricted set of MDR\n \n \n E. coli<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n populations can be maintained and spread across both community and healthcare settings in this location, contributing disproportionately to invasive disease and AMR.\n <\/jats:p>","DOI":"10.1099\/mgen.0.001122","type":"journal-article","created":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T10:15:45Z","timestamp":1698660945000},"source":"Crossref","is-referenced-by-count":1,"title":["The clinical, genomic, and microbiological profile of invasive multi-drug resistant Escherichia coli in a major teaching hospital in the United Kingdom"],"prefix":"10.1099","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3330-353X","authenticated-orcid":false,"given":"William L.","family":"Hamilton","sequence":"first","affiliation":[{"name":"University of Cambridge, Department of Medicine, Cambridge Biomedical Campus, Hills Road, UK"},{"name":"Wellcome Sanger Institute, Wellcome Trust Genome Campus, Hinxton, CB10 1RQ, UK"},{"name":"Cambridge University Hospitals NHS Foundation Trust, Cambridge Biomedical Campus, Hills Road, Cambridge CB2 0QQ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suny","family":"Coscione","sequence":"additional","affiliation":[{"name":"Cambridge University Hospitals NHS Foundation Trust, Cambridge Biomedical Campus, Hills Road, Cambridge CB2 0QQ, UK"},{"name":"University of Cambridge, Department of Medicine, Cambridge Biomedical Campus, Hills Road, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mailis","family":"Maes","sequence":"additional","affiliation":[{"name":"Cambridge Institute for Therapeutic Immunology and Infectious Disease, Jeffrey Cheah Biomedical Centre, Puddicombe Way, Cambridge CB2 0AW, UK"},{"name":"Wellcome Sanger Institute, Wellcome Trust Genome Campus, Hinxton, CB10 1RQ, UK"},{"name":"University of Cambridge, Department of Medicine, Cambridge Biomedical Campus, Hills Road, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ben","family":"Warne","sequence":"additional","affiliation":[{"name":"Cambridge Institute for Therapeutic Immunology and Infectious Disease, Jeffrey Cheah Biomedical Centre, Puddicombe Way, Cambridge CB2 0AW, UK"},{"name":"Cambridge University Hospitals NHS Foundation Trust, Cambridge Biomedical Campus, Hills Road, Cambridge CB2 0QQ, UK"},{"name":"University of Cambridge, Department of Medicine, Cambridge Biomedical Campus, Hills Road, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lindsay J.","family":"Pike","sequence":"additional","affiliation":[{"name":"Wellcome Sanger Institute, Wellcome Trust Genome Campus, Hinxton, CB10 1RQ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fahad A.","family":"Khokhar","sequence":"additional","affiliation":[{"name":"University of Cambridge, Department of Veterinary Medicine, Madingley Road, Cambridge, CB3 0ES, UK"},{"name":"Cambridge Institute for Therapeutic Immunology and Infectious Disease, Jeffrey Cheah Biomedical Centre, Puddicombe Way, Cambridge CB2 0AW, UK"},{"name":"University of Cambridge, Department of Medicine, Cambridge Biomedical Campus, Hills Road, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beth","family":"Blane","sequence":"additional","affiliation":[{"name":"University of Cambridge, Department of Medicine, Cambridge Biomedical Campus, Hills Road, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas M.","family":"Brown","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Public Health Laboratory, Cambridge Biomedical Campus, Hills Road, Cambridge CB2 0QQ, UK"},{"name":"Cambridge University Hospitals NHS Foundation Trust, Cambridge Biomedical Campus, Hills Road, Cambridge CB2 0QQ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theodore","family":"Gouliouris","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Public Health Laboratory, Cambridge Biomedical Campus, Hills Road, Cambridge CB2 0QQ, UK"},{"name":"Cambridge University Hospitals NHS Foundation Trust, Cambridge Biomedical Campus, Hills Road, Cambridge CB2 0QQ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gordon","family":"Dougan","sequence":"additional","affiliation":[{"name":"Cambridge Institute for Therapeutic Immunology and Infectious Disease, Jeffrey Cheah Biomedical Centre, Puddicombe Way, Cambridge CB2 0AW, UK"},{"name":"University of Cambridge, Department of Medicine, Cambridge Biomedical Campus, Hills Road, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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