{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T09:01:02Z","timestamp":1786438862563,"version":"build-2736575974"},"reference-count":66,"publisher":"Microbiology Society","issue":"1","license":[{"start":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T00:00:00Z","timestamp":1704240000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National Key R&D Program of China","award":["2020YFF0305002"],"award-info":[{"award-number":["2020YFF0305002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,3]]},"abstract":"Pet turtles are a well-recognized source of human salmonellosis, posing a threat to human health, particularly children who commonly keep pet turtles. To date, the genomic characteristics of \n \n Salmonella<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> among pet turtles and children has not been well described. We investigated the prevalence, antimicrobial resistance (AMR) and genomic characteristics of \n \n Salmonella<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> from pet turtles in Beijing, China. In total, 9.6\u200a% (46\/480) of pet turtles were positive for \n \n Salmonella<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> with S<\/jats:italic>. Thompson being the dominant serovar (19\/46) in 2019. Moreover, 80.4\u200a% of \n \n Salmonella<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> were multi-drug resistant (MDR) and 60.7\u200a% were resistant to ampicillin, streptomycin, sulfonamides and tetracycline (ASSuT). We further compared the genomes of S<\/jats:italic>. Thompson isolates from pet turtles (n<\/jats:italic>=19) with those from children with diarrhoea (n<\/jats:italic>=28) in the same region and year, most of which were sequence type (ST)26, with one novel ST7937 identified from a child-associated isolate. S<\/jats:italic>. Thompson isolates from children with diarrhoea exhibited less resistance than isolates from pet turtles. Most MDR isolates possessed multiple AMR genes, including the AmpC \u03b2-lactamase-encoding genes bla<\/jats:italic>\n DHA-15<\/jats:sub> and bla<\/jats:italic>\n DHA-1<\/jats:sub> which co-occurred with the IncA\/C and IncHI plasmid replicon types. To the best of our knowledge, this is the first time that the bla<\/jats:italic>\n DHA-15<\/jats:sub> gene has been detected from \n \n Salmonella<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. Several pet turtle-associated S<\/jats:italic>. Thompson isolates comprised phylogenetically close clusters with those from children with diarrhoea (<20 SNP differences). Bayesian analysis demonstrated that the Chinese ST26 S<\/jats:italic>. Thompson strains had a recent evolutionary history and evolved into two major clades, with one clade acquiring various resistant plasmids. Our findings revealed the emergence of MDR \n \n Salmonella<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> among pet turtles in China and provided evidence for the interspecies transmission of S<\/jats:italic>. 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