{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T14:57:49Z","timestamp":1771858669745,"version":"3.50.1"},"reference-count":45,"publisher":"Microbiology Society","issue":"1","license":[{"start":{"date-parts":[[2024,1,4]],"date-time":"2024-01-04T00:00:00Z","timestamp":1704326400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,4]]},"abstract":"<jats:p>Foodborne botulism is a neuroparalytic disease caused by ingestion of foods contaminated with botulinum neurotoxin (BoNT), produced by <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3901\" xlink:type=\"simple\">Clostridium botulinum<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>. In 1995 a husband and wife from Qu\u00e9bec, Canada, were hospitalized for several months with prolonged muscle paralysis after ingesting a commercial <jats:italic>p\u00e2t\u00e9 de campagne<\/jats:italic>. Examination of faecal samples from both patients and the p\u00e2t\u00e9 produced viable Group I (proteolytic) <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3901\" xlink:type=\"simple\">C. botulinum<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> type B from each of the three samples. Whole genome sequencing revealed that all three isolates contain identical <jats:italic>bont\/B5<\/jats:italic> and <jats:italic>bont\/F2<\/jats:italic> genes encoded on a plasmid. Both faecal isolate genomes were identical in chromosome and plasmid length, as well as gene content. The genome of the p\u00e2t\u00e9 isolate was nearly identical to that of the faecal isolates with the notable difference of a missing 13-gene insertion on the <jats:italic>bont\/B5<\/jats:italic> cluster disrupting the <jats:italic>ntnh<\/jats:italic> gene. Examination of the insertion revealed several mobile genetic elements that participate in recombination.<\/jats:p>","DOI":"10.1099\/mgen.0.001169","type":"journal-article","created":{"date-parts":[[2024,1,4]],"date-time":"2024-01-04T17:17:20Z","timestamp":1704388640000},"source":"Crossref","is-referenced-by-count":2,"title":["Complete genomes of Clostridium botulinum type B(F) isolates associated with a 1995 foodborne botulism outbreak from commercial p\u00e2t\u00e9 reveals a recombination event disrupting the ntnh gene"],"prefix":"10.1099","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4520-1079","authenticated-orcid":false,"given":"Richard A.","family":"Harris","sequence":"first","affiliation":[{"name":"Bureau of Microbial Hazards, Health Canada, Ottawa, Ontario, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annika","family":"Flint","sequence":"additional","affiliation":[{"name":"Bureau of Microbial Hazards, Health Canada, Ottawa, Ontario, 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