{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T02:50:22Z","timestamp":1782960622038,"version":"3.54.5"},"reference-count":89,"publisher":"Microbiology Society","issue":"7","license":[{"start":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T00:00:00Z","timestamp":1721865600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"crossref","award":["773830"],"award-info":[{"award-number":["773830"]}],"id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,7,25]]},"abstract":"<jats:p>\n                  <jats:italic>Clostridioides difficile<\/jats:italic> has significant clinical importance as a leading cause of healthcare-associated infections, with symptoms ranging from mild diarrhoea to severe colitis, and possible life-threatening complications. <jats:italic>C. difficile<\/jats:italic> ribotype (RT) 002, mainly associated with MLST sequence type (ST) 8, is one of the most common RTs found in humans. This study aimed at investigating the genetic characteristics of 537 <jats:italic>C. difficile<\/jats:italic> genomes of ST8\/RT002. To this end, we sequenced 298 <jats:italic>C<\/jats:italic>. <jats:italic>difficile<\/jats:italic> strains representing a new European genome collection, with strains from Germany, Denmark, France and Portugal. These sequences were analysed against a global dataset consisting of 1,437 ST8 genomes available through Enterobase. Our results showed close genetic relatedness among the studied ST8 genomes, a diverse array of antimicrobial resistance (AMR) genes and the presence of multiple mobile elements. Notably, the pangenome analysis revealed an open genomic structure. ST8 shows relatively low overall variation. Thus, clonal isolates were found across different One Health sectors (humans, animals, environment and food), time periods, and geographical locations, suggesting the lineage\u2019s stability and a universal environmental source. Importantly, this stability did not hinder the acquisition of AMR genes, emphasizing the adaptability of this bacterium to different selective pressures. Although only 2.4\u200a% (41\/1,735) of the studied genomes originated from non-human sources, such as animals, food, or the environment, we identified 9 cross-sectoral core genome multilocus sequence typing (cgMLST) clusters. Our study highlights the importance of ST8 as a prominent lineage of <jats:italic>C. difficile<\/jats:italic> with critical implications in the context of One Health. In addition, these findings strongly support the need for continued surveillance and investigation of non-human samples to gain a more comprehensive understanding of the epidemiology of <jats:italic>C. difficile<\/jats:italic>.<\/jats:p>","DOI":"10.1099\/mgen.0.001270","type":"journal-article","created":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T14:55:54Z","timestamp":1721919354000},"source":"Crossref","is-referenced-by-count":4,"title":["Genomic study of European Clostridioides difficile ribotype 002\/sequence type 8"],"prefix":"10.1099","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3923-5849","authenticated-orcid":false,"given":"Ines","family":"Dost","sequence":"first","affiliation":[{"name":"Present address: Landesuntersuchungsamt Rheinland-Pfalz, Koblenz, Germany"},{"name":"Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Naumburger Stra\u00dfe 96a, 07743 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2068-6081","authenticated-orcid":false,"given":"Mostafa","family":"Abdel-Glil","sequence":"additional","affiliation":[{"name":"Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Naumburger Stra\u00dfe 96a, 07743 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7389-0345","authenticated-orcid":false,"given":"S\u00f8ren","family":"Persson","sequence":"additional","affiliation":[{"name":"Statens Serum Institut, Dept. 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