{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T02:54:29Z","timestamp":1792637669267,"version":"4.5.0"},"reference-count":70,"publisher":"Microbiology Society","issue":"7","license":[{"start":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T00:00:00Z","timestamp":1721606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000272","name":"National Institute for Health and Care Research","doi-asserted-by":"crossref","award":["16_136_111"],"award-info":[{"award-number":["16_136_111"]}],"id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,7,22]]},"abstract":"<jats:p>\n                  <jats:italic>Klebsiella pneumoniae<\/jats:italic> poses a significant healthcare challenge due to its multidrug resistance and diverse serotype landscape. This study aimed to explore the serotype diversity of 1072 <jats:italic>K<\/jats:italic>. <jats:italic>pneumoniae<\/jats:italic> and its association with geographical distribution, disease severity and antimicrobial\/virulence patterns in India. Whole-genome sequencing was performed on the Illumina platform, and genomic analysis was carried out using the Kleborate tool. The analysis revealed a total of 78 different KL types, among which KL64 (<jats:italic>n<\/jats:italic>=274\/1072, 26\u200a%), KL51 (<jats:italic>n<\/jats:italic>=249\/1072, 24\u200a%), and KL2 (<jats:italic>n<\/jats:italic>=88\/1072, 8\u200a%) were the most prevalent. In contrast, only 13 distinct O types were identified, with O1\/O2v1 (<jats:italic>n<\/jats:italic>=471\/1072, 44\u200a%), O1\/O2v2 (<jats:italic>n<\/jats:italic>=353\/1072, 33\u200a%), and OL101 (<jats:italic>n<\/jats:italic>=66\/1072, 6\u200a%) being the predominant serotypes. The study identified 114 different sequence types (STs) with varying serotypes, with ST231 being the most predominant. O serotypes were strongly linked with STs, with O1\/O2v1 predominantly associated with ST231. Simpson\u2019s diversity index and Fisher\u2019s exact test revealed higher serotype diversity in the north and east regions, along with intriguing associations between specific serotypes and resistance profiles. No significant association between KL or O types and disease severity was observed. Furthermore, we found the specific association of virulence factors yersiniabactin and aerobactin (<jats:italic>P<\/jats:italic>&lt;0.05) with KL types but no association with O antigen types (<jats:italic>P<\/jats:italic>&gt;0.05). Conventionally described hypervirulent clones (i.e. KL1 and KL2) in India lacked typical virulent markers (i.e. aerobactin), contrasting with other regional serotypes (KL51). The cumulative distribution of KL and O serotypes suggests that future vaccines may have to include either ~20\u2009KL or four O types to cover &gt;85\u200a% of the carbapenemase-producing Indian <jats:italic>K. pneumoniae<\/jats:italic> population. The results highlight the necessity for comprehensive strategies to manage the diverse landscape of <jats:italic>K. pneumoniae<\/jats:italic> strains across different regions in India. Understanding regional serotype dynamics is pivotal for targeted surveillance, interventions, and tailored vaccine strategies to tackle the diverse landscape of <jats:italic>K. pneumoniae<\/jats:italic> infections across India. This article contains data hosted by Microreact.<\/jats:p>","DOI":"10.1099\/mgen.0.001271","type":"journal-article","created":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T12:17:34Z","timestamp":1721650654000},"source":"Crossref","is-referenced-by-count":12,"title":["Geographical distribution, disease association and diversity of Klebsiella pneumoniae K\/L and O antigens in India: roadmap for vaccine development"],"prefix":"10.1099","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2775-2280","authenticated-orcid":false,"given":"Varun","family":"Shamanna","sequence":"first","affiliation":[{"name":"Department of Biotechnology, NMAM Institute of Technology, Nitte, Udupi, India"},{"name":"Central Research Laboratory, KIMS, Bengaluru, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8301-3321","authenticated-orcid":false,"given":"Srikanth","family":"Srinivas","sequence":"additional","affiliation":[{"name":"Central 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