{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T19:53:18Z","timestamp":1789501998498,"version":"build-2803163510"},"reference-count":28,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2024,10,16]],"date-time":"2024-10-16T00:00:00Z","timestamp":1729036800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Defense Health Program Operation & Maintenance"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,10,16]]},"abstract":"<jats:p>Carriage of CTX-M-type extended-spectrum \u03b2-lactamase (ESBL) is rare in <jats:italic>Pseudomonas aeruginosa<\/jats:italic>. During routine surveillance of an endemic ST-621 <jats:italic>P. aeruginosa<\/jats:italic> at a large hospital, isolate MRSN 100690 carrying <jats:italic>bla<\/jats:italic>\n                  <jats:sub>CTX-M-15<\/jats:sub> was cultured from a patient (P2). This was the first detection of this ESBL in the endemic ST-621 lineage. All 1\u200a488 bacterial isolates collected from the same facility in the 12\u2009months prior to the incidence of 100\u200a690 were screened for the presence of <jats:italic>bla<\/jats:italic>\n                  <jats:sub>CTX-M-15<\/jats:sub>. A set of 183 isolates was identified, in which corresponding patient metadata was evaluated for spatiotemporal overlaps with P2. The resulting three isolates, along with 100\u200a690, were long-read sequenced using the Oxford Nanopore MinION platform to determine a potential donor of <jats:italic>bla<\/jats:italic>\n                  <jats:sub>CTX-M-15<\/jats:sub>. The screen revealed a single <jats:italic>Klebsiella michiganensis<\/jats:italic> isolate, MRSN 895358, which carried an IncA\/C2 plasmid harbouring <jats:italic>bla<\/jats:italic>\n                  <jats:sub>CTX-M-15<\/jats:sub>. Notably, the patient harbouring 895358, P1, occupied the same hospital room as P2 9\u2009months prior. Genomic alignment revealed that both isolates shared an identical 80.8\u2009kb region containing the IncA\/C2 plasmid replicon and <jats:italic>bla<\/jats:italic>\n                  <jats:sub>CTX-M-15<\/jats:sub>. This region was plasmid bound in 895\u200a358, but chromosomally bound in 100\u200a690 due to Tn<jats:italic>4661<\/jats:italic>-mediated transposition. ESBL <jats:italic>bla<\/jats:italic>\n                  <jats:sub>CTX-M-15<\/jats:sub> was acquired and subsequently integrated into the chromosome of a ST-621 <jats:italic>P. aeruginosa<\/jats:italic>, likely initiated by plasmid transfer from a <jats:italic>K. michiganensis<\/jats:italic> strain.<\/jats:p>","DOI":"10.1099\/mgen.0.001303","type":"journal-article","created":{"date-parts":[[2024,10,16]],"date-time":"2024-10-16T15:17:58Z","timestamp":1729091878000},"source":"Crossref","is-referenced-by-count":3,"title":["Tn4661-mediated transfer of bla\n            CTX-M-15 from Klebsiella michiganensis to an outbreak clone of Pseudomonas aeruginosa"],"prefix":"10.1099","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-3117-5048","authenticated-orcid":false,"given":"Katelyn V.","family":"Bartlett","sequence":"first","affiliation":[{"name":"Multidrug-Resistant Organism Repository and Surveillance Network (MRSN), Walter Reed Army Institute of Research, Silver Spring, 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