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In the SC05 culture, however, a <jats:italic>Dehalobacter<\/jats:italic> population also mineralizes dichloromethane (DCM) produced by chloroform dechlorination using the <jats:italic>mec<\/jats:italic> cassette, just downstream of its active RDase. A closed genome of this DCM-mineralizing lineage has previously evaded assembly. Here, we present the genomes of two novel <jats:italic>Dehalobacter<\/jats:italic> strains, each of which was assembled from the metagenome of a distinct subculture from SC05. A pangenomic analysis of the <jats:italic>Dehalobacter<\/jats:italic> genus, including RDase synteny and phylogenomics, reveals at least five species of <jats:italic>Dehalobacter<\/jats:italic> based on average nucleotide identity, RDase and core gene synteny, as well as differential functional genes. An integration hotspot is also pinpointed in the <jats:italic>Dehalobacter<\/jats:italic> genome, in which many recombinase islands have accumulated. This nested recombinase island encodes the active RDase and <jats:italic>mec<\/jats:italic> cassette in both SC05 <jats:italic>Dehalobacter<\/jats:italic> genomes, indicating the transfer of key functional genes between species of <jats:italic>Dehalobacter<\/jats:italic>. Horizontal gene transfer between these two novel <jats:italic>Dehalobacter<\/jats:italic> strains has implications for the evolutionary history within the SC05 subcultures and of the <jats:italic>Dehalobacter<\/jats:italic> genus as a whole, especially regarding adaptation to anthropogenic chemicals.<\/jats:p>","DOI":"10.1099\/mgen.0.001324","type":"journal-article","created":{"date-parts":[[2024,11,20]],"date-time":"2024-11-20T14:17:38Z","timestamp":1732112258000},"source":"Crossref","is-referenced-by-count":6,"title":["Pangenomic insights into Dehalobacter evolution and acquisition of functional genes for bioremediation"],"prefix":"10.1099","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1691-6892","authenticated-orcid":false,"given":"Olivia","family":"Bulka","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, 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