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This study used dual RNA sequencing to analyse the transcriptomic responses of <jats:italic>Caenorhabditis elegans<\/jats:italic> and the two <jats:italic>Shigella<\/jats:italic> species at early (10\u2009minutes) and late (24\u2009hours) stages of infection. While the nematode defence response was downregulated during both <jats:italic>Shigella<\/jats:italic> infections, only infection by <jats:italic>S. sonnei<\/jats:italic> led to downregulation of sphingolipid metabolism, cadmium ion response and xenobiotic response in <jats:italic>C. elegans<\/jats:italic>. Furthermore, <jats:italic>S. sonnei<\/jats:italic> upregulates biofilm formation and energy generation\/conservation during infection, acid resistance-related genes and biofilm regulators compared to <jats:italic>S. flexneri<\/jats:italic>. These findings highlight species-specific responses during <jats:italic>C. elegans<\/jats:italic> infection.<\/jats:p>","DOI":"10.1099\/mgen.0.001339","type":"journal-article","created":{"date-parts":[[2025,1,24]],"date-time":"2025-01-24T14:18:03Z","timestamp":1737728283000},"source":"Crossref","is-referenced-by-count":1,"title":["Shigella sonnei and Shigella flexneri infection in Caenorhabditis elegans led to species-specific regulatory responses in the host and pathogen"],"prefix":"10.1099","volume":"11","author":[{"given":"Bao Chi","family":"Wong","sequence":"first","affiliation":[{"name":"School of Science, Monash University Malaysia, 47500 Bandar Sunway, Selangor Darul Ehsan, Subang Jaya, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0794-6782","authenticated-orcid":false,"given":"Hock Siew","family":"Tan","sequence":"additional","affiliation":[{"name":"School of Science, Monash University Malaysia, 47500 Bandar Sunway, 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