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To describe invasive pneumococcal disease in the era of childhood PCV vaccination on pneumococcal bacteraemia across all ages, we collected and sequenced 979 pneumococcal blood isolates from consecutive patients with pneumococcal bacteraemia in the Gelderland area, the Netherlands, between 2000 and 2020. In total, 58% of the bacteraemia cases (<jats:italic>n<\/jats:italic>=563\/979) occurred in the elderly population. Compared to the pre-PCV period (2000\u20132005), the odds ratio for non-PCV10 bacteraemia was 17.5 (CI 9.9\u201331.6; <jats:italic>P<\/jats:italic>&lt;0.001) in the late-PCV10 period, showing an overall increase in the proportion of bacteraemia cases being caused by non-vaccine serotype pneumococci (2016\u20132020). The increase in non-PCV10 serotypes is mainly driven by an expansion of lineage global pneumococcal sequencing cluster 3 (GPSC3) expressing serotype 8, alongside the emergence of serotype 12F that was mediated by multiple lineages (GPSC32\/GPSC26\/GPSC55). Both serotypes 8 and 12F were included in the latest PCV20 formulation that is licensed to be used in children and adults in Europe. Over 20 years, we observed a low prevalence of antimicrobial resistance (AMR) as predicted by genome data. There were no significant changes in AMR prevalence after vaccine introduction (<jats:italic>P<\/jats:italic>&gt;0.05 for all comparisons). We saw a stably low prevalence of reduced penicillin susceptibility, which was observed in multiple pneumococcal lineages, with GPSC10 being the most common in the Gelderland collection, whilst GPSC1 and GPSC6 were common among the penicillin-resistant pneumococcal blood culture isolates provided by the Netherlands Reference Laboratory for Bacterial Meningitis. Comparison to global collections of GPSC10, GPSC1 and GPSC6 isolates favored the likelihood of separate introductions of penicillin-resistant isolates rather than cloncal expansion. Genomic surveillance of pneumococcal bacteraemia in this unbiased population sample in the Netherlands supports the use of higher valency PCVs, such as PCV20, especially in adults, to prevent future bacteraemia cases caused by <jats:italic>Streptococcus pneumoniae<\/jats:italic> in the Gelderland area, the Netherlands, while maintaining a low prevalence of AMR in the pneumococcal population.<\/jats:p>","DOI":"10.1099\/mgen.0.001377","type":"journal-article","created":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T15:17:29Z","timestamp":1742311049000},"source":"Crossref","is-referenced-by-count":4,"title":["Investigating two decades of Streptococcus pneumoniae bacteraemia in the Gelderland area, the Netherlands, using whole-genome sequencing"],"prefix":"10.1099","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0326-1559","authenticated-orcid":false,"given":"Ana D.","family":"Sanches Ferreira","sequence":"first","affiliation":[{"name":"Parasites and Microbes, Wellcome Sanger Institute, Hinxton, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alannah C.","family":"King","sequence":"additional","affiliation":[{"name":"Parasites and Microbes, Wellcome Sanger Institute, Hinxton, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Femke","family":"Wolters","sequence":"additional","affiliation":[{"name":"Department of Pulmonology, Catharina Hospital, Eindhoven, The Netherlands"},{"name":"Department of Medical Microbiology, Radboudumc Center for Infectious Diseases, Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heiman F.L.","family":"Wertheim","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboudumc Center for Infectious Diseases, Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bert","family":"Mulder","sequence":"additional","affiliation":[{"name":"Dicoon Laboratory, Elst & Department of Clinical Microbiology, Canisius-Wilhelmina Ziekenhuis, Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline M.A.","family":"Swanink","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology and Immunology, Rijnstate, Arnhem, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christa E.","family":"van der Gaast-de Jongh","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Laboratory of Medical Immunology, Radboudumc Community for Infectious Diseases, Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daan W.","family":"Arends","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Laboratory of Medical Immunology, Radboudumc Community for Infectious Diseases, Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina M.","family":"van Sorge","sequence":"additional","affiliation":[{"name":"Netherlands Reference Laboratory for Bacterial Meningitis, Department of Medical Microbiology and Infection Prevention, Amsterdam University Medical Center, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carel","family":"Schaars","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Pantein Maasziekenhuis, Boxmeer, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harry C. 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