{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T20:02:44Z","timestamp":1785873764193,"version":"3.56.0"},"reference-count":43,"publisher":"Microbiology Society","issue":"6","license":[{"start":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T00:00:00Z","timestamp":1750723200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,6,24]]},"abstract":"<jats:p>\n                  <jats:italic>Klebsiella<\/jats:italic> spp., the second leading cause of bacteraemia in the UK, are major nosocomial pathogens. We investigated the epidemiology of extended-spectrum <jats:italic>\u03b2<\/jats:italic>-lactamase (ESBL)-producing <jats:italic>Klebsiella<\/jats:italic> spp. in Aberdeen, Scotland and England. Twenty-four ESBL producers underwent whole-genome sequencing and typing using MLST. Sequence types were queried in the Bacterial Isolate Genome Sequence Database (BIGSdb) to assess phylogenetic relationships using Parsnp, and resistance\/virulence genes were identified by Kleborate. Plasmids were assembled with plasmidSPAdes. All isolates had the <jats:italic>bla<\/jats:italic>\n                  <jats:sub>SHV<\/jats:sub>, with three positive for <jats:italic>bla<\/jats:italic>\n                  <jats:sub>SHV-12<\/jats:sub> ESBL and two positive for <jats:italic>bla<\/jats:italic>\n                  <jats:sub>DHA-1<\/jats:sub> ESBL. The <jats:italic>bla<\/jats:italic>\n                  <jats:sub>CTX-M<\/jats:sub> was observed in 71% (33% plasmid-borne), <jats:italic>bla<\/jats:italic>\n                  <jats:sub>TEM<\/jats:sub> in 42%, with all isolates resistant to ceftazidime, cefotaxime and cefuroxime. Two isolates carried <jats:italic>mcr-9.1<\/jats:italic> on 2.5 and 203 kb plasmids. MLST identified 21 strains, with ST-13 and ST-200 being the most common. BIGSdb revealed 487 similar strains across 43 clonal groups. ST14 (34%) and ST15 (29%) were predominant multidrug-resistant strains. Nearly all isolates (99%) harboured the <jats:italic>bla<\/jats:italic>\n                  <jats:sub>SHV<\/jats:sub> (43% SHV-28, 2% SHV-12 ESBL), and 57% carried a <jats:italic>bla<\/jats:italic>\n                  <jats:sub>CTX-M<\/jats:sub>. Carbapenemases were detected in 61% of isolates, and 59% had <jats:italic>gyr<\/jats:italic>A\/<jats:italic>par<\/jats:italic>C mutations, exclusively linked to strains from England. ST432 strains from humans (resistant) and cattle (sensitive) were closely related. We also observed ST14 and ST15 (CC15) as high-risk clones thought to have evolved independently that are globally prevalent. The coexistence of <jats:italic>mcr-9.1<\/jats:italic> and plasmid ESBL <jats:italic>bla<\/jats:italic>\n                  <jats:sub>CTX-M<\/jats:sub> in <jats:italic>Klebsiella<\/jats:italic> spp. threatens the efficacy of critically important antibiotics. The close relatedness of human and cattle ST432 strains suggests potential zoonotic transmission, emphasizing the role of livestock as a reservoir for antimicrobial-resistant pathogens and the need for genomic epidemiology studies to elucidate transmission pathways and interspecies exchange. A One Health approach integrating human, animal and environmental surveillance would enhance our understanding of the molecular dynamics and evolutionary trajectory of <jats:italic>Klebsiella pneumoniae<\/jats:italic>, reinforcing the need for targeted interventions to limit the spread of antimicrobial resistance.<\/jats:p>","DOI":"10.1099\/mgen.0.001413","type":"journal-article","created":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T14:43:43Z","timestamp":1750776223000},"source":"Crossref","is-referenced-by-count":3,"title":["Molecular epidemiology of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae bloodstream infections from Aberdeen, Scotland, and their comparison to isolates from England"],"prefix":"10.1099","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1476-2108","authenticated-orcid":false,"given":"Bruno Silvester","family":"Lopes","sequence":"first","affiliation":[{"name":"School of Health and Life Sciences, Teesside University, 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