{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,15]],"date-time":"2025-08-15T00:09:48Z","timestamp":1755216588772,"version":"3.43.0"},"reference-count":47,"publisher":"Microbiology Society","issue":"8","license":[{"start":{"date-parts":[[2025,8,4]],"date-time":"2025-08-04T00:00:00Z","timestamp":1754265600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100020955","name":"GlaxoSmithKline Biologicals","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100020955","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,8,4]]},"abstract":"<jats:p>Oropharyngeal carriage of <jats:italic>Neisseria meningitidis<\/jats:italic> (<jats:italic>N.m<\/jats:italic>.) is a prerequisite for invasive meningococcal disease. As such, genomic surveillance of disease-causing carriage strains can inform targeted public health responses. However, whole-genome sequencing (WGS) from isolates is often precluded due to the high rates of culture failure for <jats:italic>N.m<\/jats:italic>. samples collected in carriage studies. This study outlines an alternative method to sequence <jats:italic>N.m<\/jats:italic>. directly from oropharyngeal specimens that enables high-resolution molecular fine typing.<\/jats:p>\n               <jats:p>We performed direct probe-capture enrichment WGS (dWGS) of <jats:italic>N.m<\/jats:italic>. on oropharyngeal specimens from the \u2018B part of it\u2019 South Australian and \u2018B part of it NT\u2019 Northern Territory meningococcal carriage studies (NCT03089086 and NCT04398849). Sequences were analysed using currently available bioinformatic tools, including the characterization of genogroup, multi-locus sequence typing (MLST), <jats:italic>Bexsero<\/jats:italic> Antigen Sequence Typing (BAST), <jats:italic>porA<\/jats:italic> and <jats:italic>fetA<\/jats:italic> type.<\/jats:p>\n               <jats:p>Sensitivity of dWGS typing compared to WGS for genogroup, MLST, <jats:italic>porA<\/jats:italic>, <jats:italic>fetA<\/jats:italic> and BAST schemes was 88.89%, 72.22%, 100%, 94.44% and 88.24%, respectively. Genogroup and <jats:italic>porA<\/jats:italic> type were more reliably characterized in unculturable samples compared to the other typing schemes assessed. Factors that influenced accurate fine typing included the amount and proportion of <jats:italic>N.m<\/jats:italic>. sequences, and the proportion of other <jats:italic>Neisseria<\/jats:italic> species in enriched sequencing libraries. An alternative phylogenetic method (phylotyping) correctly predicted the clonal complex for 93.46% of the samples assessed. These results demonstrate that dWGS enables high-resolution molecular fine typing and can be applied to unculturable samples in <jats:italic>N.m<\/jats:italic>. carriage studies.<\/jats:p>","DOI":"10.1099\/mgen.0.001464","type":"journal-article","created":{"date-parts":[[2025,8,4]],"date-time":"2025-08-04T15:16:23Z","timestamp":1754320583000},"source":"Crossref","is-referenced-by-count":0,"title":["Direct whole-genome sequencing enables strain typing of unculturable Neisseria meningitidis from oropharyngeal carriage specimens"],"prefix":"10.1099","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3873-4168","authenticated-orcid":false,"given":"Rosa C.","family":"Coldbeck-Shackley","sequence":"first","affiliation":[{"name":"Microbiology and Infectious Diseases, SA Pathology, Adelaide 5000, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Lawrence","sequence":"additional","affiliation":[{"name":"Vaccinology and Immunology Research Trials Unit, Women\u2019s and Children\u2019s Health Network, Adelaide 5000, Australia"},{"name":"Microbiology and Infectious Diseases, SA Pathology, Adelaide 5000, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6490-7707","authenticated-orcid":false,"given":"Mark","family":"McMillan","sequence":"additional","affiliation":[{"name":"Robinson Research Institute and Adelaide Medical School, The University of Adelaide, Adelaide 5000, Australia"},{"name":"Vaccinology and Immunology Research Trials Unit, Women\u2019s and Children\u2019s Health Network, Adelaide 5000, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4726-5527","authenticated-orcid":false,"given":"Caitlin A.","family":"Selway","sequence":"additional","affiliation":[{"name":"Microbiology and Infectious Diseases, SA Pathology, Adelaide 5000, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5838-7274","authenticated-orcid":false,"given":"Lito","family":"Papanicolas","sequence":"additional","affiliation":[{"name":"Microbiology and Infectious Diseases, SA Pathology, Adelaide 5000, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Turra","sequence":"additional","affiliation":[{"name":"Microbiology and Infectious Diseases, SA Pathology, Adelaide 5000, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2521-5166","authenticated-orcid":false,"given":"Helen","family":"Marshall","sequence":"additional","affiliation":[{"name":"Robinson Research Institute and Adelaide Medical School, The University of Adelaide, Adelaide 5000, Australia"},{"name":"Vaccinology and Immunology Research Trials Unit, Women\u2019s and Children\u2019s Health Network, Adelaide 5000, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5073-2272","authenticated-orcid":false,"given":"Lex E. 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