{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T18:56:55Z","timestamp":1793213815628,"version":"4.5.0"},"reference-count":77,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T00:00:00Z","timestamp":1759881600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Shanghai Sailing Program","award":["21YF1439200"],"award-info":[{"award-number":["21YF1439200"]}]},{"name":"Shanghai Outstanding Youth Training Program of the Three-Year Public Health Action Plan","award":["GWV-10.2-YQ07"],"award-info":[{"award-number":["GWV-10.2-YQ07"]}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["088786\/C\/09\/Z"],"award-info":[{"award-number":["088786\/C\/09\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Medical Research Council","award":["MR\/M501608\/1"],"award-info":[{"award-number":["MR\/M501608\/1"]}]},{"name":"Medical Research Council","award":["MR\/L015080\/1"],"award-info":[{"award-number":["MR\/L015080\/1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,10,8]]},"abstract":"<jats:p>\n                  <jats:italic>Campylobacter<\/jats:italic> is a major foodborne bacterial pathogen that has become increasingly resistant to clinically important antimicrobials. Of particular concern is the emergence of <jats:italic>erm<\/jats:italic>(B)-mediated macrolide resistance, which has been increasingly documented across <jats:italic>Campylobacter<\/jats:italic> isolates from diverse ecological reservoirs. In this study, we investigated the genomic characteristics and epidemiology of <jats:italic>erm<\/jats:italic>(B)-carrying clinical <jats:italic>Campylobacter<\/jats:italic> isolates from Shanghai, alongside a globally representative dataset of all publicly available strains. Among clinical isolates obtained from a diarrhoeal outpatient surveillance programme between 2020 and 2023 in Shanghai, China, 16% (80\/500) were erythromycin-resistant, with 23.8% (19\/80) testing positive for <jats:italic>erm<\/jats:italic>(B). The genomes of these isolates were sequenced to identify <jats:italic>erm<\/jats:italic>(B) gene alleles. Phylogenetic analyses, pairwise comparisons of core and accessory genomes and examination of shared alleles revealed horizontal gene transfer as the predominant mechanism driving the transmission of <jats:italic>erm<\/jats:italic>(B) between isolates from various sources. Poultry was identified as a key reservoir for human infections caused by <jats:italic>erm<\/jats:italic>(B)-positive <jats:italic>Campylobacter<\/jats:italic> isolates. Comparative pangenome analyses of <jats:italic>erm<\/jats:italic>(B)-positive and negative isolates identified multiple accessory elements associated with <jats:italic>erm<\/jats:italic>(B) acquisition, among which the IS607 family transposon-associated <jats:italic>tnpB<\/jats:italic> gene exhibited sequence and structural homology to functional progenitors of CRISPR-Cas nucleases. These findings expand our understanding of the epidemiology of <jats:italic>erm<\/jats:italic>(B)-mediated macrolide resistance in <jats:italic>Campylobacter<\/jats:italic> and underscore the urgent need for enhanced antimicrobial stewardship in poultry production and targeted surveillance programmes to curb the spread of resistance.<\/jats:p>","DOI":"10.1099\/mgen.0.001528","type":"journal-article","created":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T16:16:45Z","timestamp":1759940205000},"source":"Crossref","is-referenced-by-count":1,"title":["Genomic epidemiology and the evolution of erm(B)-mediated macrolide resistance in Campylobacter"],"prefix":"10.1099","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4444-1807","authenticated-orcid":false,"given":"Fen","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Microbiology, Shanghai Municipal Center for Disease Control and Prevention, Shanghai, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frances M.","family":"Colles","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Oxford, Oxford, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seungwon","family":"Ko","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Oxford, Oxford, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayuan","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Shanghai Municipal Center for Disease Control and Prevention, Shanghai, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6901-3203","authenticated-orcid":false,"given":"Samuel K.","family":"Sheppard","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Oxford, Oxford, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Shanghai Municipal Center for Disease Control and Prevention, Shanghai, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00006-15"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2024.8583"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268814003185"},{"key":"R4","volume-title":"Antibiotic Resistance Threats in the United States, 2019","year":"2019"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.14760"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.554784"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1145581"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1186\/s44280-023-00029-5"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2015.11.005"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt492"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkac086"},{"key":"R12","doi-asserted-by":"crossref","first-page":"e02615","DOI":"10.1128\/AAC.02615-17","article-title":"Report of erm (b) + campylobacter jejuni in the united states","volume":"62","author":"Chen","year":"2018","journal-title":"Antimicrob Agents Chemother"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.02240"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.13046"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01124-21"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00153-19"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.ARBA-0013-2017"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.03039-14"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00416-17"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02799-06"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1101\/gr.186072.114"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-11-595"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.12688\/wellcomeopenres.14826.1"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.1.14-23.2001"},{"key":"R26","article-title":"National Antimicrobial Resistance Monitoring System for Enteric Bacteria (NARMS). 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