{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T15:55:04Z","timestamp":1789055704516,"version":"build-2803163510"},"reference-count":97,"publisher":"Microbiology Society","issue":"11","license":[{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Swiss Government Excellence Scholarships for Foreign Students"},{"name":"Swiss National Science Foundation Fund","award":["310030_212837"],"award-info":[{"award-number":["310030_212837"]}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"crossref","award":["Grant ID 323630_221864\/1"],"award-info":[{"award-number":["Grant ID 323630_221864\/1"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,4]]},"abstract":"<jats:p>\n                    The anaerobic bacterium\n                    <jats:italic>Clostridium perfringens<\/jats:italic>\n                    is commonly found in the intestinal tract of humans and animals. However, there are marked differences in virulence between isolates and toxinotypes, which largely depend on various virulence factors produced by these strains. Studying\n                    <jats:italic>C. perfringens<\/jats:italic>\n                    genomes has been limited by fragmented assemblies from short-read sequencing and incomplete clinical metadata. Here, we present a high-quality collection of 236 isolates from animal hosts that underwent detailed pathomorphological examination. From 220 of them, genomes were generated by PacBio long-read sequencing, enabling comprehensive, structural level analysis of their virulome, plasmids, conjugative elements and biosynthetic gene clusters (BGCs). One hundred forty isolates were collected from animals with signs of\n                    <jats:italic>C. perfringens<\/jats:italic>\n                    -associated enteric disease, 32 from animals with no signs of\n                    <jats:italic>C. perfringens<\/jats:italic>\n                    -associated disease and 64 from healthy animals. An additional 383 publicly available\n                    <jats:italic>C. perfringens<\/jats:italic>\n                    complete or draft genomes were included for comparative analyses. We discovered 2 previously undescribed pore-forming toxin (PFT) homologues and 11 novel haemolysin and aerolysin-type PFT variants. Both findings expand the known spectrum of the\n                    <jats:italic>C. perfringens<\/jats:italic>\n                    virulome. Moreover, we defined two novel putative plasmid conjugative loci in a collection of 888 here assembled and circularized plasmids. They may facilitate HGT, supporting the dissemination of virulence and metabolic traits. We predicted 414 BGCs that were frequently toxinotype specific and encoded centrally for a bacteriocin that could help their carriers to outcompete other bacteria in shared environments. Furthermore, comparative analysis of\n                    <jats:italic>C. perfringens<\/jats:italic>\n                    plasmids revealed three distinct clusters based on their conjugation system. Altogether, these findings significantly expand the landscape of animal-associated\n                    <jats:italic>C. perfringens<\/jats:italic>\n                    through high-quality genome data and highlight novel virulence-associated features that provide a foundation for future studies of this important pathogen.\n                  <\/jats:p>","DOI":"10.1099\/mgen.0.001545","type":"journal-article","created":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T12:17:02Z","timestamp":1762258622000},"source":"Crossref","is-referenced-by-count":1,"title":["Genomic investigations in animal enteric disease-associated Clostridium perfringens"],"prefix":"10.1099","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6481-0825","authenticated-orcid":false,"given":"Jan","family":"Franzen","sequence":"first","affiliation":[{"name":"Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland"},{"name":"Department of Infectious Diseases and 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